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烟酰胺腺嘌呤二核苷酸

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Nicotinamide adenine dinucleotide phosphate oxidase is a major source of reactive oxygen species production in many nonphagocytic cells.

还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶是许多非吞噬细胞中反应性氧自由基产生的主要来源。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明:猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同,均属不稳定类酶蛋白;保守结构域氨基酸序列具有高度的同源性,均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序;均有信号肽,属分泌型蛋白质;属于跨膜的疏水性蛋白,均有5个跨膜结构域;无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件;三维结构预测都呈"V"字形;猪和牛HMGR基因的进化关系最近。

At last, adsorbent and covalent Ag nanoparticles modified electrodes were prepared by adsorbent and covalent method, respectively. The electrochemical behaviours of SCN-, brilliant cresyl blue and nicotinamide adenine dinucleotide on modified electrode were studied by Cyclic voltammetry, differential pulse voltammograms and electrochemical impedance spectroscopy.

最后,用吸附法和共价键合法制备了吸附型和共价自组装银纳米修饰电极,采用循环伏安法、示差脉冲伏安法和交流阻抗谱等电化学方法研究了SCN-、灿烂甲酚蓝和还原型烟酰胺腺嘌呤二核苷酸的电化学行为。

In 1984, Kirkman and Gaetani (1984) reported tightly bound NADPH in BLC, which then was also detected in the X-ray structure of BLC (Fita and Rossmann, 1985a). In the course of various sequencing projects a broad spectrum of catalase sequences from organisms of all kingdoms appeared in the public databases in the last 17 years e.g.

Vainshtein等获得了在3.5A 低等真核细胞中的过氧化氢酶的第一个电子云图,同时Eventoff 也得到了在2.5A处,用X-ray详细分析附面层控制图,1984年Kirkman 和报道了 Gaetani BLC中的紧密结合的还原型烟酰胺腺嘌呤二核苷酸磷酸

The results indicate that Ca(superscript 2+) and GA3, single or combined application treatments, promote cucumber seed germination treated with P. harmala alkaloid, increase germination rate, germination vigor and index; as well as α-amylase, protease, lipase, isocitratelyase, nicotinamide adenine dinucleotide kinase and Ca(superscript 2+)-ATPase activities; and GA and CaM content in cucumber seeds; but decrease abscisic acid content. The synergistic effects of combined Ca(superscript 2+) and GA3 on increasing GA and decreasing ABA are oberseved compared with Ca(superscript 2+) treatment.

结果表明,Ca(上标 2+)和GA3单一及复合处理可促进骆驼蓬生物碱处理的黄瓜种子的萌发,使黄瓜萌发率、发芽指数和活力指数提高,萌发种子的α-淀粉酶、蛋白酶、脂肪酶和异柠檬酸裂解酶活性及赤霉素和钙调素含量增加,烟酰胺腺嘌呤二核苷酸激酶和Ca(上标 2+)-ATP酶活性增强,脱落酸含量降低;且Ca(上标 2+)和GA3复合处理对GA含量的提高和ABA含量的降低与Ca(上标 2+)单一处理比较呈显著的加成作用。

The glucose-6-phosphate dehydrogenase increased during overw intering,indicating that reduced condition of cell might keep the cell in pr oper function even in cold temperature,and also for cryoprotectant synthesis.The changes in trehalase activity showed that low activity was for keeping higher tre halose content and high activity for removing it in the spring.

越冬期间,体内葡萄糖-6-磷酸脱氢酶活性增高所产生的还原型烟酰胺腺嘌呤二核苷酸磷酸,可为细胞在亚低温状态下发挥正常功能以及体内抗冻保护性物质的合成提供还原动力,同时通过调节体内海藻糖酶活性来维持越冬期间较高含量的海藻糖和移除春季体内累积的过多的海藻糖。

The prestent invention discloses the cDNA sequnce of a new human reduced NADH: ubiquinone oxidoreductase 14 KDa subunit (NADH-CoQ14KDa subunit). The protein coded with the sequence is the homolog of ox's NADH-CoQB14.5b subunit.

本发明提供了一种新的人还原型烟酰胺腺嘌呤二核苷酸-辅酶Q氧化还原酶14千道尔顿亚基(NADH:ubiquinone oxidoreductase 14 kDa subunit,简称为&NADH-CoQ 14 kDa亚基&的cDNA序列,该序列编码的蛋白是牛NADH-CoQB14.5b亚基的同系物。

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