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After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象,提取细菌基因组DNA,用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切,回收大小为1~5Kb的DNA片段,将其连接入原核表达载体pET-28a-c,最后转化宿主菌,结果成功地构建了基因表达文库,为后续的基因筛选工作奠定基础;另外,本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计,以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板,进行PCR扩增反应,结果表明成功地获得两个新基因:23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸),并进一步做了不同物种之间的分子系统发育分析,构建了基于23S rRNA基因的邻接法系统发育树,阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Originally, the SARS crisis that has impacts upon the multi-facet areas such as society, economy, politics and culture geminated from one simple Mobile Short Message which can dated back to Feb, 8th 2003:"Deadly Flu Outbreak in Guangzhou". Promulgated southward at an increased rate of 1 to 5 million messages per day and underpinned by the intervention of Internet and the marginalized inter-personal communication web, the rumors of SARS witnessed the belated evolvement of traditional media which contribute nothing to the ado but noise.

追本求源,SARS危机给中国的社会、经济、政治与文化等各方面所带来的影响,始自2003年2月8日的一条手机短信:&广州发生致命流感&;当这条只有8个汉字的短信以每天递增100-500万条的速度悄悄在南方蔓延时,作为新兴传播势力的网络传媒迅速介入,通过非主流的民间网络信息发布的推波助澜,推动了富有影响力的传统传媒对SARS事件发出了迟到的、但却嘈杂而响亮的声音。

85 And 119486/79. They are a novel type of antibiotic having both the wide antibacterial spectrum and high safety of penicillin and cephem antibiotics belonging to beta -lactam antibiotics, as well as the potent antibacterial activity and high beta -lactamase stability of carbapenem antibiotics.Sodium-(5R, 6S)-6-[-1-hydroxyethyl]-7-oxo-3- [-2-tetrahydrofuryl]-4-thia-1-azabicyclo [3.2.0] hept-2-ene-2-carboxylate 5/2 hydrate (faropenem sodium, hereinafter referred to as compound 1) is currently used as an oral drug for various infectious diseases and is reported to show potent antibacterial activity against not only methicillin-sensitive Staphylococcus aureus, Streptococcus pyrogenes and Streptococcus pneumoniae but also gram-positive bacteria for which conventional beta -lactam drugs have proved ineffective such as penicillin-resistant pneumococci, oral staphylococci and enterococci, also showing a wide antibacterial spectrum covering gram-negative bacteria such as Haemophilus influenzae and anaerobic bacteria such as the genus Bacteroides, which activity is due to its novel skeleton penem ring (Kagaku Ryoho no Ryoiki The Field of Chemotherapy, Vol.

他们是一种新型的抗生素都具有广泛的抗菌谱和高度的安全青霉素和cephem抗生素,属于β-内酰胺类抗生素,以及作为强大的抗菌活性和高β-内酰胺酶稳定的碳青霉烯类antibiotics.sodium -( 5 R , 6 S )-6 -[ - 1 -羟乙基] - 7 -氧- 3 - [ - 2 - t etrahydrofuryl] - 4 -硫杂- 1 -氮杂双环[ 3 。2.0]庚- 2 -节能- 2 -羧酸5 / 2水合物(法罗培南钠,以下简称为化合物1 )是目前用来作为口服药物,为各种传染病和报道,以显示强大的抗菌活性,不仅对甲氧敏感的金黄色葡萄球菌,链球菌pyrogenes和肺炎链球菌,但也克阳性菌为常规β-内酰胺类药物已证明无效,如青霉素耐药pneumococci ,口腔葡萄球菌和肠球菌,也显示出广泛的抗菌谱,包括革兰阴性菌如流感嗜血杆菌和厌氧菌如属杆菌,这活动是由于其新颖的骨架青霉烯环((化学ryoho没有ryoiki领域的化疗),第13卷,第10号,第74-80 , 1997年)。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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