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流产胎

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In organ transplantation, it has been shown that the anergic T cells may be generated in vivo and in vitro, and can be transferred as the tolerant cells to prevent allograft rejection.

研究母-胎免疫耐受机制,不仅对动物及人类自然流产的防治具有重要意义,而且对移植免疫和肿瘤免疫的研究亦将产生深远的影响。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

How are you, my wife of 27 years old, in May this year to do test-tube baby, pregnant 60 days of vaginal bleeding and abortion (99 years due to rupture of cornual pregnancy had surgery, the side of tubal cut off the side and pass inspection has been impeded, it is to do test-tube).

试管不成功后,又自然怀孕,阴道出血如何保胎你好,我妻子27岁,于今年5月份做试管婴儿,孕60天时阴道大出血而流产(99年因宫角孕破裂做过手术,一侧输卵管切断,一侧检查一直通而不畅,故做试管)。

Olfactory bulb was abstracted to culture the human fetal OECs, and the monoplast suspension were made and cultured in the uncoated culture flask.

选用流产胚胎标本24份,胎龄4~6月,由西安交通大学第二医院和陕西省妇幼保健院产科提供。

Peripheral blood Oestradiol level and CD4+CD25+Treg quantity were reduced, which may be resulted in down-regulating the immune function of maternal-fetal tolerance, and mediate recurrent spontaneous abortion finally.

外周血雌二醇水平和CD4+CD25+Treg数量减低可能下调母胎免疫耐受功能,参与介导反复自然流产的发生。

Four-month-old fetus samples were provided by Department of Gynaecology and Obstetncs of Second Affiliated Hospital of Soochow University. The puerperant and her relatives all signed informed consent for extraction.

实验材料:4个月胎龄的流产胎儿由苏州大学附属第二医院妇产科提供,产妇及家属均签署知情同意书,实验经医学伦理委员会批准。

One hundred metaphases were counted with 45, XO, inv (7) in 15﹪ and 46, XX, inv (7)in 85﹪.

染色体臂间倒位能引起流产和畸胎,应作产前诊断。

Result: The first five pathogenic factors of PPROM are infection, breech presentation, multiplets, premature delivery and miscarriage, and the syndrome of pregnancy hypertension.

结果:感染、臀位、多胎、早产流产史及妊高征占据PPROM病因的前五位。

To examine rates of spontaneous and therapeutic abortions, rates of stillbirths, birth weight, and gestational age at birth.

审查和治疗自发性流产率,死胎率,出生体重,胎龄和出生。

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