母体发芽的

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。