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Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Methods Twenty-three cases of OSAS patients proved by polysomnography were examined with MRI. The cross-sectional area of retropalatal region, retroglossal region, epiglottal region and the lateral parapharyngeal fat pad of RP region was calculated. The thickness of bilateral and posterior pharyngeal wall and other indices were also measured. 30 nonsnoring age-matched normal subjects were selected as the control group. 15 patients underwent turbo field echo T1-weighted imaging during sleep. The images of RP, RG, and EPG were analyzed. Results In oSAS group, the cross sectional area of RP and RG regions was smaller than that of the control group.

应用MRI对23例经多导睡眠监测(polysomnography, pSG)确诊的OSAS患者及30例无打鼾的健康成人进行上气道检查,于轴面图像测量软腭后区(retropalatal region, RP区)、舌根后区(retroglossal region, RG区)和会厌区(epiglottal region, EPG区)的气道截面积,各区前后、左右径及其比值,各区咽侧壁、咽后壁软组织厚度,RP区咽旁间隙(lateral parapharyngeal fat pad,LPFP)截面积,于正中矢状面图像测量软腭厚度、长度及截面积,对两组各项检查指标进行统计学分析。

BACKGROUND: Posterior tibial nerve block has traditionallybeen performed in the para-medial malleolar area without nervestimulator guidance.

背景:在没有神经刺激器引导的情况下,胫后神经阻滞传统是在旁正中踝区域完成的。

She led us to a table big enough for four in the center ofthe most crowded area of the dining floor.

她把我们带到用餐区里人最多的地方的正中,一张足以坐下四个人的桌子旁。

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