棉铃虫

In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

ABSTRACT Heliothis armigera nuclear polyhedrosis virus , due to its special pestiferous effect on Heliothis armigera and being innocent to human, livestock and environment, has been a commercial bioinsecticide widely in the world for years.

棉铃虫核型多角体病毒是我国最早注册的杆状病毒杀虫剂，到1999年，约有100，000公顷棉田用棉铃虫核型多角体病毒的生物制剂防治害虫。

Assulta and the induction of GST activity by nicotine and capsicine in H. assulta provided partial supports for the hypothesis that diet breadth was governed by detoxification abilities. The results also indicated that two sibling species had different strategies of adaptation to toxic substances: H. assulta saves expenditure of nutrients and energy by restricting their countermeasures to a few potentially toxic compounds. H. armigera, by contrast, must be prepared to counter a wider range of potential toxins. 5. Compared with parental species, hybrids of reciprocal hybridization had significant difference in feeding preference for hot pepper.

结果显示，广食性的棉铃虫在中肠羧酸酯酶和谷胱甘肽-S-转移酶活性方面高于寡食性的烟青虫，但烟青虫谷胱甘肽-S-转移酶活性可以被寄主植物内的烟碱和辣椒素所诱导，表明解毒酶活性在一定程度上影响着植食性昆虫的食性广度；同时也暗示着二近缘种对有毒的次生物质采取不同的适应策略：烟青虫有选择地对付种类较少的植物次生物质以减轻营养和能量的消耗；棉铃虫则必须对付存在于其寄主植物内的种类繁多的有毒次生物质。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

The compound eyes of cotton bollworm showed higher sensitivity under similar conditions; for one insect, the compound eyes were more sensitive to light in the evening than at the daytime, with dark eyes had higher sensitivity than bright eyes;(4) The comparison of the transition between different adaptative state at different time showed that the compound eyes of cotton bollworm showed more obvious daily rhythm.

在相同条件下，棉铃虫复眼表现出更强的敏感性；同种昆虫复眼夜间比白天敏感，暗眼比亮眼敏感；（4）对不同测试时间复眼明暗状态转化的比较表明，棉铃虫复眼的ERG反应具有更强的昼夜节律性。

Results show that the insect community structure in the Bt fields differed sharply from that in the CV fields. The populations of the Lepidopterous pests, such as cotton bollworm, corn borer, diamond bollworm and cotton leaf caterpillar, decreased significantly in the BT fields, while the populations of non-target pests with piercing-sucking mouthparts, such as leaf bugs, cotton spider mites, cotton aphids and whiteflies, increased obviously. Much more predators, such as spiders and ladybirds, and much less parasitiods of Helicoverpa armigera were observed in the BT fields.

结果表明，与常规棉田相比，Bt棉田棉铃虫、玉米螟、金刚钻、棉小造桥虫等鳞翅目害虫种群数量显著降低，而盲蝽象、红蜘蛛、棉蚜、烟粉虱等非靶标刺吸式害虫种群数量显著增加；蜘蛛、瓢虫等捕食性天敌种群数量显著增加，而棉铃虫的寄生性天敌种群数量则显著下降。

Yu C G%Ma M%Ding T%Hetrick F,Hsu H T Biochemical characterization and time course analysis of Lymantria dispar nuclear polyhedrosis virus with monoclonal antibodies Canadian Journal of Microbiology , 1992,null

王根 棉铃虫核型多角体病毒多角体蛋白基因的克隆和核苷酸序列分析病毒学报， 1997，(1)吴柏春 1990中国棉铃虫核型多角体病毒VH A273毒株多角体蛋白某些理化特性的研究病毒学杂志， null，null

Two sibling species Helicoverpa armigeraH(u|¨bner and H.assulta are serious crop pests in China.They have the similar morphological,biological and ecological characterstics,as well as they can even hybridize with each other.Their host plant ranges, however,are quite different.The former is a typical polyhpagous species,and its host plant range including at least more than 60 crop species such as cotton,corn,wheat,soybean, tobacco,and tomato,and 67 wild plant species from about 30 plant families including Malvaceae,Solanaceae,Gramineae,Leguminosae,etc.

可同域发生的棉铃虫Helicoverpa armigeraH(u丨¨bner和烟夜蛾H.assulta是铃夜蛾属两近缘种昆虫，二者具有相似的生物学和生态学习性，甚至杂交可育，但它们的寄主范围却截然不同，棉铃虫是多食性昆虫，烟夜蛾则是典型的寡食性昆虫。

The cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) is an important cotton pest.

棉铃虫Helicoverpa armigera属于鳞翅目夜蛾科实夜蛾亚科棉铃虫属，是一种世界性的重要经济害虫。

The results showed that Lufenuron possessed high ovicidal activity on H.armigera 1-day eggs at 50 mg/L,eggs mortality rate was 87.30%;LC50 of stomach poisoning of 2nd ～ 5st instar larvae were 0.7434 mg/L,1.9669 mg/L,2.0592 mg/L and 2.6945 mg/L with leaf dipping method,respectively.

结果表明，虱螨脲具有较强的杀卵作用，在50 mg/L浓度下，棉铃虫1日龄卵死亡率达到87.30%；对棉铃虫2~5龄幼虫具有较高的胃毒活性，其LC50分别为0.743 4 mg/L、1.966 9 mg/L、2.059 2 mg/L和2.694 5 mg/L。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。