核碎裂

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

It is better to use a neutron or proton rich projectile with atomic number notfar away from the expected exotic nuclei because the difference in the fragmentationcross section induced by neutron or proton rich and stable projectiles becomes smallwith the increase of the distance between the produced fragment and the projectile. Measurements of 〓 for 〓,〓 and 〓 at intermediate energieshave been performed on the Radioactive Ion Beam Line in Lanzhou.

所选的丰中子或丰质子炮弹最好离所需产生的丰中子或丰质子奇异核比较近，因为随着炮弹与产生的核的距离的增加，丰中子或丰质子炮弹与稳定的炮弹的碎裂反应产生奇异核的产生截面的差别变小。

Cooperative effect of 5-Fu and TRAIL was also determined. The structure feature of apoptosis cell such as karyopyknosis, karyorrhexis and apoptotic body was found lightmicroscopically and electron- microscopically.

HE染色光镜观察和电镜观察发现经TRAIL作用后的细胞具有核浓缩、碎裂、凋亡小体形成等凋亡的形态特征。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖，使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长，光镜下见细胞突起变短，细胞由梭形变成椭圆形，核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

Its characteristic changes are monocellular occurrence,pyknosis,karyorrhexis and the formation of apoptotic body.

其特点是单细胞发生，胞核浓缩、碎裂，核碎块被胞膜包裹，形成特异性的&凋亡小体&；细胞器多以萎缩变化为主。

After 48h exposure to 50mg/L ox-LDL, under the fluorescent microscope partial cells Showed typical apoptotic morphological changes such as nacarat fluorescent body shrinking and fragment of the nucleus. At the same time, the cellular cholesterol content also increased obviously, and many lipid droplets accumulated in cells.

RAW264.7细胞经50mg／L ox-LDL处理48h后，AO／EB染色显示明显的细胞凋亡形态学特征，出现核固缩、碎裂，同时细胞内胆固醇含量显著增加(由76.3μg／mg蛋白上升至368.5μg／mg蛋白)，油红O显示细胞内有大量的脂滴存在。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Simultaneously the disappearance of microvilli, vacuolization of cytoplasm, dispersion of nucleus and appearance of apoptotic body were observed under transmission electron microscopy. All these morphological changes are hallmarks of apoptosis.

凋亡细胞经荧光染色后表现为细胞核固缩、碎裂；透射电镜下观察发现细胞微绒毛丧失，胞内出现空泡，胞核解体及形成凋亡小体等典型的凋亡特征。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下，与对照组相比，经姬姆萨染色观察，西咪替丁、尼扎替丁处理的SW480细胞，先发生皱缩，体积变小，异形性减少，细胞变圆数目增多，细胞核变小，染色变淡，核仁数量减少，部分可见染色质边集现象；细胞间连接疏松，随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后，SW480细胞全部变圆，细胞核碎裂，固缩微核形成，部分不再贴壁。

Resembled to control oocytes, MAPK was inactivated after cytoplasts activation. However, after activation the cytoplasts showed fragmentation while pronuclei were formed in the control oocytes. This indicated that nuclei play important role in maintainence of oocytes normal morphology.

成熟胞质的活化与卵母细胞相似，在活化后MAPK被迅速灭活，只是胞质在活化处理后均发生碎裂，而卵母细胞活化后形成原核，这表明核在细胞形态维持中起重要作用。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。