未成熟的

To evaluate the role of mature cumulus cells from oocyte-cumulus complex in in-vitro maturation and establish a new culture technique which is convenient to carry out.

探讨人类成熟卵丘细胞在未成熟卵母细胞体外成熟培养中的作用，并建立一种简易的实施技术。

After being dispersed by simply beat upon with syringe and adherent culture, the mature cumulus cells from mature OCCs in COH cycles, together with growth factors in the follicular fluid or extraneously supplemented, could promote the IVM of immature oocyte.

来自控制性促排卵周期的成熟卵丘细胞经简易吹打分散后贴壁培养，可能能协同卵泡液中或外加的生长因子，促进未成熟卵母细胞的体外成熟，而本研究技术简易有效，可用于挽救促排卵周期的未成熟卵。

Sperm nucleus immaturity rate in each AlCl3 group increased significantly (P.01) with a dose dependent manner.

精子核荧光染色试验中，高、中、低剂量AlCl3染毒组未成熟精子率均高于阴性对照组，差异有统计学意义(P.01)；且未成熟精子率随着AlCl3染毒剂量的升高而增加。

Objective To clarify the relationship between oocyte maturation and mitochondria distribution by investigating the distribution of mitochondrial in immatured and matured human oocytes.

目的研究人未成熟和成熟卵母细胞中线粒体的分布，进一步阐明卵母细胞成熟和线粒体分布的关系。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件，消融人肝细胞癌株SMMC-7721细胞悬液，分为五组：A、对照组，B、45℃10min组，C、50℃3min组，D、54℃3min组，E、60℃即刻组，消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤，间隔1周，右侧腹股沟皮下接种瘤块作为观察瘤，成瘤后分为四组：A组，对照组，不行治疗；B组，微波组，仅微波消融小鼠消融瘤；C组，成熟DC组，微波消融小鼠消融瘤，并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞；D组，未成熟DC组，微波消融小鼠消融瘤，并于消融瘤周注射未成熟树突状细胞。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

Maturity of Lower Cretaceous source rock, researched by the authors, is closely relative to the deposition thickness of the Tertiary and the Quaternary. It shows that source rock at the depth of 4,000-4,400m is low mature, and 4,400-5,200m is in the peak of oil generating. Correspondingly, the source rock of the Zhonggou Formation is immature; the Upper Xiagou Formation is low mature; and the Lower Xiagou Formation and the Upper Chijinpu Formation are in the peak of oil-generating; and the Lower Chijinpu Formation is at the stage of generation of condensate-wet gas with high maturity. There is no source rock reaching the overmature stage of dry gas at present.

可溶有机质转化率、镜质体反射率、热解、生物标志物特征等参数表明：青南凹陷中部的下白垩统烃源岩生油门限深度为4000m（相应的镜质体反射率为0.65％），埋深4000～4400m为低成熟阶段，埋深4400～5200m为成熟生油高峰阶段，因此中沟组烃源岩未成熟，下沟组上部烃源岩低成熟，下沟组下部和赤金堡组上部烃源岩处于成熟生油高峰阶段，赤金堡组下部烃源岩处于高成熟的凝析油—湿气阶段，目前尚无烃源岩达过成熟干气阶段。

At 21 DAG, the newly established cambium cell started periclinal division, which would result in the production of phloem outwards and xylem inward. The 11-23 immature xylem cell layers beneath the surface continued their differentiation into mature xylem cells.

表面下7-11层未成熟木质部细胞在第7-14 DAG左右脱分化形成新的形成层。21 DAG，再生形成层开始平周分裂，产生新的韧皮部和木质部。11-23层未成熟木质部细胞继续分化为成熟木质部。

Result The cultured DC from mouse bone marrow displayed the typical morphological characteristics of DC. Immature DC, which had high expression in CD11c and low expression in MHCⅡ and CD86, could phagocytize antigen. Mature DC, which had high expression in CD11c, MHCⅡ and CD86, could stimulate allogenic mixed lymphocyte proliferation.

未成熟DC的细胞表型为CD11c、MHCⅡ、CD86，具有极强的抗原吞噬能力；未成熟DC经LPS刺激培养后可转变为成熟DC，细胞表型为CD11c、MHCⅡ、CD86，可显著刺激同种异体混合淋巴细胞增殖。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母细胞中主要存在Ⅷ突变型，而野生型表达相对较少，成熟后卵母细胞中的Ⅷ突变型蛋白明显增加。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。