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The operative area was examined with light microscope, electronic microscope, and the proliferating cell nuclear antigenwas detected. Results In CsA group, intraocular pressure was significantly lower than that in NS group(P<0.05), and more functional filtering blebs were observed. Less fibroplasia and proliferative cells, less bioblast and less active caryotin in fibrocyte were found in CsA group than that in NS group. No serious mcomplicaton was found in both group.

结果 环孢霉素A组眼压控制多较理想,与对照组间的差异有统计学意义(P<0.05),大多数形成功能性滤过泡,光镜下见极少量纤维组织增生,电镜下见纤维细胞线粒体少,染色质功能不活跃,增殖细胞阳性率低,与对照组差异有统计学意义(P<0.05),且没有浅前房、视网膜损害等严重并发症。

At 1st, 7th,14th,and 30th day after surgery, filtering blebs, intraocular pressure, cornea and ocular fundus were observed.the operative area was examined with light microscope, electronic microscope, and the proliferating cell nuclear antigenwas detected. results in csa group, intraocular pressure was significantly lower than that in ns group(p<0.05), and more functional filtering blebs were observed. less fibroplasia and proliferative cells, less bioblast and less active caryotin in fibrocyte were found in csa group than that in ns group. no serious mcomplicaton was found in both group.

结果 环孢霉素a组眼压控制多较理想,与对照组间的差异有统计学意义(p<0.05),大多数形成功能性滤过泡,光镜下见极少量纤维组织增生,电镜下见纤维细胞线粒体少,染色质功能不活跃,增殖细胞阳性率低,与对照组差异有统计学意义(p<0.05),且没有浅前房、视网膜损害等严重并发症。

Results Expression of Fn in tumor tissues is significantly altercated and correlated to the biological Characteristics of gastric cancer.

结果 胃癌组织内纤维连接蛋白改变的特征为基底膜纤维连接蛋白减少,而间质内纤维连接蛋白增多,且与胃癌的生物特性有密切关系。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩,管壁增厚呈空虚网状,生精细胞层次减少,间质细胞增生,但仍见有精子生成;高剂量组可见到精子呈异型性改变,细胞核浓缩深染,且随着摄入时间延长改变愈趋明显;F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩,间质增生明显,20个月时肾小球萎缩纤维化;F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生,淋巴母细胞增生活跃,20个月时脾小体减少,生发中心稀疏;F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润,晚期骨髓有核细胞减少,脂肪细胞增加。

The purpose of this study was to investigate the effect of different doses o f dietary fiber of Tremella fuciformis Berk on lipid metabolism and inte stinal physiology in rats fed diets containing 1% cholesterol and 0.2% cholica cid. Forty-two male Wistar rats were randomly divided into six groups in a 2× 3 factorial design and were fed diets containing different doses (0%、5% or 10 %) of dietary fiber of TFB, with and without antibiotics 0.7g/100g of Nebaci tin (bacitracin: neomycin sulfate = 2:1,w/w) for 4 weeks. The parameters analyz ed included liver and serum lipid concentration , serum and caecal content of short chain fatty acids ,fecal neutral steroids and total bile acids content.T he results showed that rats fed the diet with antibiotics had lower levels ofs hort chain fatty acids in serum and caecal content than rats fed diet withouta ntibiotics.

中文摘要本研究乃欲探讨在有高胆固醇饮食条件下,摄取不同量的白木耳膳食纤维及添加抗生素对大白鼠脂质代谢及肠道生理的影响。42只 Wistar 品系雄鼠(重约260g),随机分为六组,每组饲料中均添加 1%胆固醇及 0.2%胆酸,并以添加或不添加抗生素[ 0.7g Nebacitin(bacitracin:neomycin sulfate=2:1,w/w)/100g dry matter] 、白木耳膳食纤维量(0%、5%或 10%)作 2×3 factorial 之实验设计,实验期四周,分析血清及肝脏中脂质浓度,血清及盲肠内容物短链脂肪酸浓度,以及粪便中总中性固醇,总胆酸的含量。

Results :①The MVs were formed by osteoblast of the secret type、dentinoblast and process;② There was a mature process of the MVs in the extracellular matrix.The appearance of the hydroxyapatite was a major mature feature;③ HAP formed initially in the MVs entered into collage matrix and eventually mineralized;④ The shifting sign of HAP from MVs to the surface of collage fibril was observed.

结果:①MVs是由分泌型成骨细胞、成牙本质细胞及其突起芽生进入胶原基质;②MVs在细胞外胶原基质中有一个逐渐成熟的过程,并以羟基磷灰石(hydroxyapatite,HAP)晶体的出现为其主要特征;③HAP在MVs内形成、增多,最终进入胶原基质并进一步沉积、钙化;④部分胶原原纤维表面沉积的HAP 晶体与MVs分泌的晶体有明显的移形迹象。

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象,线粒体少,胞核畸形,胞内有吞噬颗粒,细胞周围胶原纤维较少TEM×5200

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明,处理后4周,全周角膜缘上皮板层手术切除,中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化,未发生睑球粘连,角膜基质胶原纤维完整未见溃疡、穿孔等病变,细胞印迹学检查为结膜表型,可作为实验性角膜缘干细胞移植的病理模型;全周角膜缘上皮板层手术切除,中央角膜上皮用生理盐水擦除的5只试验家兔,有2只为结膜表型,另3只为角膜表型,观察期内结果不稳定;半周角膜缘上皮板层手术切除,中央角膜上皮层用生理盐水擦除的5只试验家兔,角膜表面透明,全部为角膜表型;直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔,有4只角膜基质胶原纤维断裂、溶解,并伴有严重的溃疡、穿孔、睑球粘连等病变,不能用于移植试验,另1只角膜表面透明,未见结膜和新生血管长入,细胞印迹学检查为角膜表型。

Whereas osteoporosis is unassociated with bone pain, osteomalacia has been associated with isolated or generalized bone pain.39,40 The cause is thought to be hydration of the demineralized gelatin matrix beneath the periosteum; the hydrated matrix pushes outward on the periosteum, causing throbbing, aching pain.7 Osteomalacia can often be diagnosed by using moderate force to press the thumb on the sternum or anterior tibia, which can elicit bone pain.7,40 One study showed that 93% of persons 10 to 65 years of age who were admitted to a hospital emergency department with muscle aches and bone pain and who had a wide ariety of diagnoses, including fibromyalgia, chronic fatigue syndrome, and depression, were deficient in itamin D.41

然而,骨质疏松症与骨痛无关联,而软骨病则与局部性或全身性骨痛有关。其原因被认为是骨膜下已去矿质化的胶原基质上发生的水合反应,水合的胶原基质在骨膜上向外扩张,引起阵痛。软骨病可通过以拇指适度挤压胸骨和胫骨前方以引起骨痛感。一项研究显示10岁到65岁中有93%的人向医院急诊室承认有肌肉疼痛和骨痛症状,他们还有一些其他症状包括纤维肌痛、慢性疲劳综合征、抑郁等,该研究显示他们都缺乏维生素D。本人已认领该文第9、10两部分,48小时后若未提交译文,请其他战友自由认领。本人已认领该文11、12部分编译,48小时后若未提交译文,请其他战友自由认领。本人认领第十三部分,48小时内交稿请战友纠错!

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups(P<0.01).The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group(P<0.01,P<0.05).The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面,差异均有显著性(P<0.01);IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性(P<0.01); MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达,差异具有显著性(P<0.01,P<0.05),MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达;TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

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