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The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因;一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因;片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源,但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性;片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源,而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性;另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

For the group representation, this paper improves the known method of determining the irreducibility. Instead of solving equations and checking every combination of the roots, we calculate the value of a polynomial.

对于自由群的表示,本文首先将不可约性的判别方法,由已有文献中解方程,并对方程的根作组合的方法,改进为直接计算某个多项式的值的方法,并进一步给出区分绝对不可约性和非绝对不可约性的判别准则。

The results showed:among the 7 isolates, five isolates of HY3、GY1-3、ZJ1-1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示:7个菌株中,其中5个菌株HY3、GY1-3、ZJ1-1、HP1、FC3的菌落形态相同:不规则形状、流体、粘性、不透明、表面光滑;另外2个菌株93B、GN1菌落形态相同:不规则形状、干燥、不透明、表面粗糙;用7个菌株接种剪根桉树苗,发病情况非常明显,而自来水对照实验中桉树苗却不发病;无EPS菌悬液培养桉树剪根苗,发病率也很明显,但是相比原菌液,则发病率有不同程度的下降;从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株,用它们配制菌悬液培养桉树苗,设置剪根和不剪根两个处理,每个处理设置五个重复,数据分析结果显示:对于剪根苗,上部和中部、上部和下部的含菌量有显著的差异,中部和下部含菌量差异不显著;带根苗,上部、中部、下部含菌量彼此之间差异显著;不管是剪根苗还是带根苗,木质部和韧皮部含菌量之间的差异都非常显著;上中下与木韧交互作用中,含菌量差异非常显著;最后筛选出来的强弱2个菌株HY3和93B,它们的纤维素酶活性分别为:1.955ug/和1.288ug/,具有显著的差别;果胶酶的活性分别为:1.325 ug/和1.24ug/,没有显著的差别,而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著,分别为:7.08×10-8ug/cell和5.17×10-8ug/cell。

Second we use excitability medicine to provocat the nerve,s causality discharage,after 1 to 2 minutes,perfuse the brain slice with a certain concentration of puerarin to be abaissement,in result,we find that some kinds concentration(such as 1:1000) of puerarin have prominen depressant effect to the provocated discharage of the hippocampal nerves.

之后,通过在电活性较好的脑片上先进行兴奋性药物的诱发,待诱发性放电出现后1-2分钟再灌注一定浓度的葛根素进行实验观察,结果表明,一定浓度的葛根素(如1:1000)对海马神经元的诱发放电活动有显著的抑制作用。

To probe into either the attractiveness to or avoidance from phylloxera of grape rootstocks and cultivars with different tolerance, as well as into the volatile components from their roots, the method of invitro root segment culture was used to investigate the selectivity of Phylloxera viticola Fitch to the roots of grape rootstocks '5BB' and '140Ru' and cultivar 'Kyoho'.

为了探讨不同抗性水平砧木及品种对根瘤蚜吸引及驱避效应以及鉴定挥发性物质组分,采用离体根段培养法测定葡萄根瘤蚜Phylloxera viticola Fitch对砧木5BB,140 Ru及栽培品种巨峰的选择性,发现根瘤蚜对栽培品种巨峰的根段具有很强的选择性,至接种后3d,67.13%的根瘤蚜优先选择巨峰;而对5BB和140 Ru的选择性却很差,分别有15.29%和17.58%的根瘤蚜选择5BB及140 Ru。

This paper has studied the physiological and biochemical changes during the period of forming strong buds of tree peony, and expect to provide academic basis for extending its view value and economic benefit . The main results are as the following:1 Under the field condition, Characteristics of photosynthesis of tree peony are very regular. From before blooming to the prophase of abducting buds that after blooming ,that is April,May and June,the net photosynthetic rate of tree peony is higher than the other period .It indicates that this period is very important for accumulating photosynthetic products of tree peony . So, if we want to get quality flower of tree peony ,it is very important to strengthen the management of tree peony cultivation and to ensure provide the fertilizer and water.2 From the middle ten days of June, with the time elapsing and with the environment conditions worsening, the characteristics of photosynthesis of tree peony decline deeply. But it is different among different tree peony breeds. For example , cv. Wu Long Peng Sheng can also has higher net photosynthetic rate, it indicates that cv. Wu Long Peng Sheng is super than the other two tree peony breeds when adapting the environment conditions and cv. Wu Long Peng Sheng has wide prospect to extend .3 The main factors of effecting the characteristics of photosynthesis are stomatal factor and non-stomatal factor ,that is the ability of photosynthesis of leaf cells. Under the environment of high light intensity in midsummer, if overshadow tree peony properly, then it can slow the declining of chlorophyll content, and increase the net photosynthetic rate and benefit for the accumulating of photosynthetic products.4 The content of soluble sugar in the root of tree peony is the highest among different organs during the period of forming strong buds . According to analyse ,we believe that the soluble sugar will compose starch then, and provide the solid base of material for the growth and development next year. It provide us strong academic basis of adopting the method of cultivating root and protecting root under thefield environment conditions .In other words ,it will help to increase the health level of the whole plant of tree peony and will be beneficial to forming quality tree peony flower if we strengthen the management of root. 5 To tree peony, the endogenesis hormone is important for its growth and development .In the prophase ,that is about the blooming of tree peony and the mid-summer ,IAA and ZR contents are higher and GA content is lower and its changes are very little, and the content of ABA is decreasing . This indicates that IAA and ZR act the important promote function for growth and development of tree peony in this period, and the function of GA is not important. Bur in the period of anniversary senescence of tree peony , the content of IAA and ZR begin to decrease ,while the ABA content has a certain rebound .T

本研究对几个牡丹品种壮芽形成过程中的某些生理生化变化做了研究,以期了解牡丹生长发育过程中生理生化的变化规律,探讨大田栽培条件下提高牡丹花质量的栽培措施和管理技术,为提高牡丹的观赏价值和经济效益提供理论依据,结果如下:1 大田栽培条件下,牡丹光合特性在生长发育过程中表现出极强的规律性,牡丹各品种的光合速率从牡丹开花前,到牡丹花谢后的花芽诱导期前期(6月10日之前),也就是在4、5、6月份,各牡丹品种的净光合速率相对与其他时间都维持在比较高的水平,这说明,在这段时间,是牡丹光合产物积累的重要时期,因此,要保证得到高质量的牡丹花,在这段时间加强对牡丹的栽培管理,保证这个时期的肥水供应十分重要。2 从6月中旬后,随时间的推移,环境条件的恶化,牡丹的光合性能剧烈下降,但是不同牡丹品种间仍有差异,比如,乌龙捧盛仍可维持较高的光合水平,这说明了乌龙捧盛这个牡丹品种对于环境的适应性较之其他牡丹品种高,有着广泛的推广前景。3 影响牡丹光合性能的主要因子是气孔因素和非气孔因素即叶肉细胞的光合能力,在盛夏高温高光强的环境条件下,对牡丹进行适当遮荫,能缓解牡丹叶绿素水平的下降,适当提高Pn,有利于光合产物的积累。4 可溶性糖在牡丹各品种的壮芽形成过程中,根中可溶性糖的含量在牡丹各部位中始终维持最高水平,分析认为这些可溶性糖在根系中进一步合成淀粉等储藏性物质,为来年牡丹的生长提供雄厚的物质基础,这为我们在大田栽培条件下对牡丹采取养根护根的栽培措施提供了有力的理论依据,即在大田栽培条件下,加强对牡丹根系的管理,有助于提高牡丹整株的健壮程度,有益于形成高质量的牡丹花。5内源激素对牡丹的生长发育调节作用是十分明显的,在前期,即开花前后和盛夏,IAA和ZR含量维持在比较高的水平,GA含量低且变化不明显,ABA含量逐渐降低,说明了IAA和ZR对牡丹在这段时间内的生长发育起到重要的促进作用,GA的作用不是很明显,ABA可能在高温高光强下有明显的升高,也说明了ABA的确有一些促进作用,但是到了牡丹周年衰老期,IAA和ZR的含量开始下降,ABA含量却有了一定程度的反弹,说明了IAA和ZR的作用开始降低,ABA起到了促进衰老的作用

Of 92 acutely dissociated dorsal root ganglion cells recorded by current patch-clamp technique, 34% were discharged by FA application with the mean onset latencies of the first action potential being (367.34+/-32.96) s. All the FA-sensitive cells were identified as nociceptor cells by their distinguishable features of AP including longer duration, existence of a hump on the repolarizing phase, and longer after-hyperpolarization of APs.

运用电流钳记录的92个背根神经节细胞中,大约有34%的中、小细胞对甲醛溶液敏感,产生动作电位的发放,其产生动作电位的潜伏期是(367.34±32.96) s,并且单个动作电位复极化相具有伤害性感受器细胞的特征性,如动作电位间期较长、复极化相有&驼峰&以及较长的后超极化相。

Results The clinical observation of 180 patients with entirely developed root apex of acute and chronic apical periodontitis shown that there was no significant difference comparing erythromycin ethylsuccinate-metronidazole-CP with FC in root canal sterilization, therefore, the irritability and poisonousness of the paste can be reduced by using erythromycin ethylsuccinate-metronidazole-CP instead of FC.

为了清除髓腔病源刺激物,促进根尖病变愈合,目前国内大多用甲醛甲酚进行根管消毒。甲醛甲酚属化学制剂,虽具有强烈的杀菌作用,但亦具有刺激性、毒性和抗原性等,而琥珀酸红霉素和甲硝唑在临床上已被证明对厌氧菌有明显疗效,对于根管内的消毒杀菌作用如何,研究报道不多。

RESULTS:①The myelography and CTM revealed that factors contributing to DLSS included not only zygapophysial joint hyperplasy, hypertrophic ligamentum flavum and intervertebral disc protrution which resulted in degenerative lumbar spinal stenosis, but also zygapophysial joint semiluxation and pedicle shift.

结果:①脊髓造影与CT脊髓造影的表现:DLSS对硬膜囊或神经根的压迫较退行性腰椎管狭窄症复杂,除与关节突肥大增生、黄韧带肥厚、椎间盘退变突出有关外,还与关节突关节半脱位、椎弓根移位压迫神经根等因素有关。

The seeds of Adzuki bean were irradiated by 60 Co γ ray of 0~700Gy.The results showed that:(1)The sub\|lethal dose(LD 50 )was 389Gy and the early seedling leaf at M\-1 showed incised,chlorotic,yellow,mosaicand curly edge.The absorbed dose was related with the rate of misshaped leaf.Chromosomal aberration, chromosome bridge and fragment of M\-1 in the tip were observed.If the seed was irradiated with 400Gy,the rate of abnormal cell at the root was more than 10%.The relationship between pollen fertility and irradiation dose was negative correlated.

本试验研究结果表明:60 Coγ射线辐照小豆种子的半致死剂量平均为 3 89Gy ,且M1生长初期叶片性状有明显的叶面缺刻、叶色缺绿、叶形不对称、叶缘卷曲、花叶、黄叶等致变现象,各种畸型叶出现频率与辐照剂量大小有关;M1根尖细胞中有多种染色体畸变类型,以染色体桥和断片频率较高,辐照剂量在 40 0Gy以上时根尖细胞异常率达 1 0 %以上;植株花粉育性与辐照剂量呈极显著负相关,京农 2号植株花粉育性达 5 0 %时的辐照剂量为 3 78Gy。

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