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晶体蛋白

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The results indicatethat the expression of Cyt1A alone could result in the cytolysis of host bacterial cells,and both P20 and Cry11A could prevent bacterial cells from cytolysing caused byCyt1A protein.

为了进一步探讨P19和P20对Cry11A和Cyt1A相互作用的影响,本研究构建了表达P19、P20、Cry11A和Cyt1A不同组合的七个Bt重组菌株,分别从目的蛋白表达、营养细胞生长、芽胞和晶体形成、杀虫毒力等方面进行了研究。

Results show that silicon of high crystalline quality is homogeneously distributed inside the voids of silica template.

研究结果表明:Si在SiO2微球空隙内具有较高的结晶质量,填充致密均匀;通过控制沉积条件,可控制硅的填充率;制备的硅反蛋白三维光子晶体在近红外区(1.4μm左右)具有明显的光学反射峰,表现出光学带隙效应,测试的光学性能与理论计算基本吻合。

Over 98% of purified DOC-1R proteins was finally got for structural and functional research. Mass Spectrum identified that the exact molecular weight of DOC-1R is 14091.23 Da; Dynamic light scattering assay showed that its homogenesis was over 99.0%.

质谱分子量测定显示DOC-1R的分子量为14091.23 Da,与理论分子量基本一致;动态光散射实验显示蛋白均一性高达99.0%,可用于晶体生长;采用悬滴气相扩散法筛选,在多个条件下得到了DOC-1R的微晶。

Crystallogra- phy requires very pure and highly concentrated solutions of the protein of interest.

晶体衍射需要很纯、浓度很高的目的蛋白溶液。

R Phycoerythrin ; Time resolved ; Polarized fluorescence ; Chromophore orientation

R-藻红蛋白晶体;时间分辨;偏振荧光;色素取向

Other factors, such as physical chemistry and protein inhibitors of crystal growth, also play a role.

其他因素,如物理化学和晶体形成蛋白抑制剂,也同样起一定作用。

And under a suitable condition, dark brown short rhombohedron crystals of ΔnifE Av1 were obtained and identified for the first time.

首次获得了ΔnifE Av1的深棕色短斜四棱柱晶体,并对其蛋白组成进行了鉴定。

Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from FeMoco-deficient MoFe protein (ΔnifE Av1) purified from a nifE deleted mutant DJ35 of Azotobacter vinelandii Lipmann grown in NH3-limited medium.

从限氨固氮培养基中培养棕色固氮菌缺失nifE的突变种DJ35中,分离纯化得到缺失FeMoco的钼铁蛋白(ΔnifE Av1)。在一定条件下结晶得到深棕色短斜四棱柱晶体。

This muant strain also lost the ability of sporulation and production of crystal. At the same time, the production of ICPs is severely decreased by SDS-PAGE analysis.

该突变株也丧失了产生芽胞和晶体的能力,SDS-PAGE分析表明突变株G03的杀虫蛋白产量也非常低。

The application of the third generation synchrotron radiation has greatly impact ...

在技术和方法方面,近年来第三代同步辐射的应用与一些新方法的结合,可用极小的晶体测定极大的结构,使某些膜蛋白及亚细胞器的精确结构测定成为可能。

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