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The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

TABLE 7-4:OUTLANDS EFFECTS ON SPELLS AND ABILITIES Distance form Spire Impeded Spells Limited Spells Other Effects 1,200 mi. None None None 1,100 mi. 9th None None 1,000 mi. 8th-9th None None 900 mi. 7th-9th 9th All creatures gain immunity to poison 800 mi. 6th-9th 8th-9th Psionic spell -like abilities don't function 700 mi. 5th-9th 7th-9th Positive and negative energy can't be channeled 600 mi. 4th-9th 6th-9th Supernatural abilities don't function 500 mi. 3rd-9th 5th-9th Access to the Astral Plane prohibited 400 mi. 2nd-9th 4th-9th Divine powers of demideity rank and lower annulled 300 mi. All 3rd-9th Divine powers of lesser rank and lower annulled 200 mi. All 2nd-9th Divine powers of intermediate rank and lower annulled 100 mi.

表 7-4:在外域上法术及能力的效果距离无极尖峰的距离阻碍法术限制魔法其它效果 1200英哩无无无 1100英哩 9级无无 1000英哩 8级-9级无无 900英哩 7级-9级 9级所有生物获得对毒素免疫能力 800英哩 6级-9级 8级-9级心灵类法术能力无效化 700英哩 5级-9级 7级-9级正能量和负能量无法进入 600英哩 4级-9级 6级-9级超自然能力无效化 500英哩 3级-9级 5级-9级无法接触星界位面 400英哩 2级-9级 4级-9级半神及以下等级神力被取消 300英哩所有 3级-9级微弱及以下等级神力被取消 200英哩所有 2级-9级中等及以下等级神力被取消 100英哩所有所有所有等级的神力被取消

Java Core API for Celestial Calculation - Java algorithms to calculate celestial ephemerides, for astronony, and celestial navigation.

这个项目提供了用于计算星历表的Java算法,供天文学和天体导航学使用。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪,通过机械分割和组织消化法获取脂肪基质细胞,在内皮细胞培养液中培养7d后,经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征,再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤,1μmol/L地塞米松,10μmol/L胰岛素,200μmol/L吲哚美星)定向诱导7d,形态学观察和油红O特殊染色鉴定诱导后的细胞。

Objective To observe the effect and safety of intravesical instillation of hydroxycamptothecin and pirambicin for preventing postoperative recurrence of the superficial bladder cancer.

目的探讨羟基喜树碱和吡柔比星组合膀胱灌注预防表浅性膀胱癌术后复发的效果和安全性。

Objective:To explore the relationship of negative schizophrenic cognitive function improvement with HVA and 5-HIAA, Method:Thirteen negative schizophrenic patients were treated with clozapine for 8 weeks,Memory,attention,executive function were assessed separately with Waise memory scale,cancellation test and Wisconsin card sorting test before and after treatment,and cerebrospinal fluid HVA and 5-HIAA levels were determined with HPLC-ECD, Results:Part of short memory was improving with decreasing of HVA or increasing of 5-HIAA,while significant correlation were not found between improvement of memory quotient,attention,executive function and change of HVA,5-HIAA, Conclusion:The great part of negative schizophrenic cognitive function improvement has marked correlation with change of DA and 5-HT

目的:探讨阴性精神分裂症认知功能的改善与HVA和5-HIAA变化的关系。方法:对13例阴性精神分裂症在氯氮平治疗前和治疗8周后分别采用韦氏成人记忆量表,数字划消测验和威斯康星卡片分类测验(Wisconsin card sorting test,WCST)评估其记忆、注意及执行功能,采用高效液相色谱-电化学检测测定其治疗前和治疗后脑脊液中DA代谢产物高香草酸和5-HT代谢产物五羟吲哚乙酸(5-HIAA)的浓度。结果:部分短时记忆随HVA降低或5-HIAA的增高而改善。而总的记忆商数,注意及执行功能的改善与HVA和5-HIAA的变化无明显相关。结论:大部分阴性精神分裂症认知功能的改善与DA和5-HT浓度变化无明显相关。

ABSTRACT: OBJECTIVE To study the stability and in vitro cytotoxicity of two kinds of dextran-epirubicin conjugates.

目的 研究两种方法制备的葡聚糖-表柔比星偶合物的稳定性及体外细胞毒活性。

Using these x,y measurements (determined to a fraction of a pixel) and the comparison star coordinates (taken from a suitable reference catalogue ), the program should then do a least-squares plate-constants solution to derive the unknown coordinates of the minor bodies.

利用这些像素坐标以及比较星的坐标(从一些合适的比较星星表获得),软件将进行一个LSPC操作来计算小行星的坐标,很多软件做不到这一点。

At the bottom of Figure 25, a geocentric ephemeris (using Bretagnon and Simon 1986) gives the apparent positions of the naked-eye planets during the eclipse.

在图25的底部,星历表显示了在日食期间可以用肉眼观测到的行星的位置。

In this study, six campaigns around Anping tide gauge, Tainan, were successfully performed and the collected GPS buoy data were processed with four types of precise ephemeris provided by IGS, including final product, rapid product, ultra-rapid product and ultra-rapid product with the use of PPP technique. Comparing the PPP results with DGPS, the differences reach 3~5 cm in the horizontal and 10 cm in the vertical with final product; 6~8 cm in the horizontal and 15 cm in the vertical with rapid product; 15~20 cm in the horizontal and 30~40 cm in the vertical with ultra-rapid product; 2~3 m in the horizontal and 3~4 m in the vertical with ultra-rapid product. In addition, the collected data were also processed by DGPS techniques using different reference stations to analyze the effect of various baselines. The results show that accuracy degrades when the baselines increase.

本研究在台南安平潮位站旁进行6次GPS浮标施测,首先利用与GPS浮标距离不同之GPS参考主站来进行差分定位,分析基线距离对GPS浮标定位成果的影响,由实验结果可得出基线越长则定位准确度越低;再以IGS提供之最终产品、快速产品、超快速产品(Ultra-Rapid product)之观测部分observed half、超快速产品(Ultra-Rapid product)之预估部分四种不同发布延迟时间的精密星历与精密时表改正资料对GPS浮标进行精密单点定位解算,与传统差分相对定位方法定位结果进行比较后,得出使用最终产品之平面方向均方根误差(Root Mean Square Error, RMSE)可达3~5公分,而高程方向均方根误差可达10公分;快速产品之平面方向均方根误差可达6~8公分,而高程方向均方根误差可达15公分;超快速产品观测部分之平面方向均方根误差可达15~20公分,而高程方向均方根误差可达30~40公分;超快速产品预估部分之平面方向均方根误差可达2~3公尺,而高程方向均方根误差可达3~4公尺。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严,以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆,把帆卷起,系住。

Therefore, positively advances the interest rate marketability reform is one of current our country finance reform important tasks.

因此,积极推进利率市场化改革是当前我国金融改革的重要任务之一。