无结果

objective: to investigate diagnosis and treatment choice of detrusor hypoactivity with impaired contractile function on voiding function in benign prostatic hyperplasia.methods:the clinical date of 48 bph patients with dhic were analyzed,including clinical diagnosis,urodynamic parameters,treatment and follow-ups.results:37 patients with detrusor instability and over 40 cmh2o bladder pressure were treated successfully by turp.the time of indwelling suprapubic punctural cystostomy cathter was(1~8)weeks.11cases without di and under 40 cmh2o bladder pressure were treated by suprapubic punctural cystostomy,accepted the test of urodynamics after(1~3)months.7 cases with di were treated successfully by turp,4 cases without di were indwelled suprapubic punctural cystostomy cathter all life.conclusion:dhic worsens the voiding function of the patients with bph,and the test of urodynamic is helpful to diagnose and could provide more convinced evidences for treatment of patients.

分析膀胱过度充盈所致逼尿肌损伤，引起膀胱逼尿肌收缩无力良性前列腺增生48例患者的临床资料，包括诊断，尿动力学检查，治疗及随访结果。结果：37例经尿流动力学检查，诱发存在逼尿肌不稳定，伴膀胱内压达40 cmh2o以上，采取turp，术后留置膀胱造瘘管（1~8）周后全部拔除，排尿通畅。11膀胱内压始终未达到40 cmh2o，且在膀胱注水充盈过程中无逼尿肌不稳定出现，行膀胱造瘘后出院，（1~3）个月后经尿动力学检查复查膀胱逼尿肌收缩力，其中7例膀胱逼尿肌收缩功能有恢复，行经尿道前列腺汽化电切术治愈；剩余4例膀胱逼尿肌收缩功能几乎无任何改善。长期留置膀胱造瘘治疗。结论：良性前列腺增生导致膀胱过度充盈所致逼尿肌损伤，引起膀胱逼尿肌收缩无力患者，术前应用尿流动力学检查对膀胱逼尿肌损伤程度进行认真分析，可进行有针对性的治疗。

A modified homogeneous flow model was introduced to calculate the flow field in RH degasser,thus getting rid of some earlier methods that are unavailable to be generalized,such as reducing the computation region or presetting the distribution of gas volume fraction.

分别对有、无旋转磁场作用时的RH系统进行了模拟计算。模拟结果表明：流场和脱碳过程与现有文献中实验结果相符合；当旋转磁场作用后，磁感应强度为0.04 T时，初期脱碳速度比无旋流作用时增加了约15%，这是由于旋流增加了系统的搅拌能，提高了脱碳反应速率。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

The Mannich-type addition of O , O'-dialky phosphite to 2-fluorobenzaldehyde and 4-trifluoro- methylaniline was carried out in one pot by means of adopting three kinds of green synthetic methods, microwave irradiation, ionic liquid and free solvent-catalyst condition. According to the results, the synthetic method was optimized without catalyst and solvent at 100 ℃ and 15 min, and it was economic, convenient, high yields, timesaving and environment friendly.

采用微波辐射﹑离子液体和无溶剂无催化剂三种绿色合成方法，分别进行了以亚磷酸酯、邻氟苯甲醛和对三氟甲基苯胺为原料的类Mannich一锅法合成反应研究，经合成筛选，优选出控制反应温度100 ℃、无溶剂和无催化剂加热反应15 min的合成方法，实验结果表明该方法经济、简便、产率高、反应时间短及对环境友好。

Results the positive rate of hpv6.11,hpv16.18,hpv6.11/16.18 were 52（25.4%）、45（22.0%）、26（12.7%） respectivelys. logistic regression analysis and multiple factor variance analysis showed clearing degree and h2o2 concentration promoted hpv6.11/16.18 infection and speeded infection process. but pyocyte, mycetes, neuraminidase had no dependability with hpv6.11/16.18 infection.

结果 205例疑似尖锐湿疣感染患者检出hpv6.11、hpv16.18、hpv6.11/16.18阳性例数为52、45、26.logistic回归和多因素方差分析结果表明，清洁度和h2o2浓度与hpv6.11/16.18感染和加速感染进程密切相关，与脓细胞、霉菌、唾液酸苷酶并无相关性。ph值、白细胞、霉菌、滴虫、线索细胞、白细胞酯酶与hpv6.11/16.18感染虽无相关性，但在引起hpv6.11/16.18感染方面起到重要作用。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Results:The serum sE-cadherin levels in health control did notvary throughout the menstrual cycle.The preoperative serum sE-cadherin levels were higher in patients with endometriosis than inhealth control.No differences were found between enometriosis patientswith differnt stage and clinical symptom.The preoperative serum sE-cadherin levels were higher in patients with ovarin cancer than in bothhealth control and patients with endometriosis.

研究结果：1、正常育龄妇女血清中sE-cad水平在月经周期中无明显改变〓，且血清中sE-cad水平与年龄不相关；2、内异症患者术前血清中sE-cad水平高于正常对照组〓，不同期别内异症患者血清中sE-cad水平无显著差别〓，有无痛经的内异症患者血清中sE-cad水平无显著差别〓；3、卵巢癌患者术前血清中sE-cad水平分别高于内异症患者组及健康对照组〓。

Results All patients had no bladder and urethra injury, no retropubic hematoma, After removal of catheters, 47 patients successfully voided without incontinence, detrusor instability to various degrees developed in 8 patients and were relieved by expectant treatment. 1 suffered urination difficulty and cured by releasing tension sling. All patients were followed up for 3 to 12 months (mean 6 months), neither recurrence of SUI nor lower urinary tract obstruction was found. No graft rejection and inflammation occurred.

结果 所有患者无膀胱、尿道损伤，无耻骨后血肿形成；47例患者术后拔除尿管均能自行排尿并满意控尿，8例患者出现不同程度不稳定膀胱症状，经对症处理缓解。1例出现排尿困难，经松解张力线后缓解，随访3~12个月，平均6个月，无SUL症状复发及排尿困难，无移植物排斤、感染发生。

These 375 patients had a median age of 50.57±10.46(range,19-72) with 87.4%HBsAg positive and 4.3%anti-HCV antibody positive;The apparent peak incidence age was 40～60 years old,and the ratio of male to female was 10.7:1;The 3 and 5 year postoperational survival rate were 52%and 38%;The tumour numbers(p=0.000),tumor size(p=0.025),histological pattern (p=0.000),nuclear features(p=0.000),differentiation(p=0.001) and vascular invasion(p=0.000) were significantly correlated with prognosis.The postoperational survival time of thin trabeculae pattern,compact pattern and pseudoglandular pattern were significantly longer than that of thick trabeculae, scirrhous pattern,and solid patternp＜0.009the postoperational survival time of 1 and 2 grade based on nuclear features were significantly longer than that of 3 and 4 grades(p=0.000The small cell variant,osteoclast-like giant cell variant, and spindle cell variant were mainly composed of thick trabeculae pattern and solid pattern,which were significantly different from that of clear cell variant.

结果1。本组资料显示肝细胞癌发病年龄19～72岁，平均50.57±10.46岁，发病高峰年龄40～60岁，男女比例为10.7：1；HBsAg87.4%，anti-HCV抗体4.3%；术后3年生存率为52%，5年生存率为38%；肿瘤数目(p=0.000)、肿瘤大小(p=0.025)、组织学结构类型(p=0.000)、核分级(p=0.000)、分化程度(p=0.001)及血管浸润均(p=0.000)与预后明显相关；其中组织学结构类型中细梁状型生存时间与致密梁状型和腺样型无明显区别(p＞0.05)而明显高于粗梁状型、实性型和硬化型(p≤0.009)，硬化型生存时间与实性型之间无明显区(p＞0.05)而明显低于其余各型p≤0.006核分级1级与2级生存时间无明显区别(p＞0.05，核分级3级与4级生存时间无明显区别(p＞0.05)，而核分级3级生存时间明显低于2p=0.000小细胞型、巨细胞型和梭形细胞型主要由实性型和粗梁状型组织学结构类型构成，明显不同于透明细胞型(主要由细梁状型和粗梁状型构成(p≤1.006)。2。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P＞0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果：1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内，细胞形态为星状，细胞突起与支架管壁粘附；MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P＞0.05；12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P＞0.05)。

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At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。