提纯

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

The purity of polypeptide is now extractable through the available biological technology,which carries significant and effective influences for organic physiological functions.

通过生物技术分离提纯的多肽，对机体生理功能的影响是明显和有效的。

Lanthanides belong to the sixth cycle of the periodic table Ⅲ B family, are all solid metal, they are La, Ce, Pr, Nd, Pm, Sm, Eu, Gd, Tb, Dy, holmium, erbium, thulium, ytterbium, and lutetium were 15 kinds of metals and their periphery are two layers of electronic 5d16s2, while the peripheral third layer f orbital but never zero to 14, so the external electronic structure of 4f0-145d16s2, and therefore these elements in oxidation number are to 3, very close chemical properties, extraction, when it is difficult to separate them they are mostly multi-element mineral mix, more difficult to increase refining, and their atomic number of 57-71, one by one to increase the number of protons the nucleus, and thus extranuclear electron of gravity is also growing, so that atomic radius decreases with increasing atomic number Z, which makes lanthanides contraction, ionic radius is also reduced, which also makes Zr, Hf, Nb and Ta, Mo and W, the atom and ionic radius very close to each other they also determine a similar nature, resulting in difficult separation, also the tribe Ⅷ of the fifth cycle, ruthenium, rhodium, palladium, and the sixth cycle, osmium, iridium, platinum is very similar in nature, thus The six elements, said platinum group elements.

镧系元素属于周期表第六周期中ⅢB族，全部是固体金属，它们是镧、铈、镨、钕、钷、钐、铕、钆、铽、镝、钬、铒、铥、镱和镥共十五种金属元素，它们的外围两层的电子均是5d16s2，而外围第三层的f轨道电子却从零增加到14个，所以外围电子结构为4f0－145d16s2，而使这些元素氧化数均为＋3，化学性质极为接近，提炼时很难分开，它们的矿石又大多是多种元素混在一起的，更增加提纯难度，它们的原子序数为57－71，核内质子数逐个增加，因而对核外电子引力也不断增强，使原子半径随原子序数Z增加而减小，这就使镧系元素收缩、离子半径也缩小，这也使得Zr和Hf，Nb和Ta、Mo和W等原子和离子半径都很接近、也决定了它们彼此性质类似，以致难以分离、也使第Ⅷ族中第五周期的钌、铑、钯和第六周期的锇、铱、铂在性质上极为相似，因而称这六个元素为铂系元素。

Technology for upgrading ferronickel and the possible separation of nickel and cobalt from ferronickel are also discussed.

介绍了生产超纯镍铁以及多种镍铁基合金的试验数据，同时也讨论了镍铁提纯工艺以及从镍铁中分离铜、钴的可能性。

Carries on the synthesis experiment, the productdepuration, through the highly effective liquid phase chromatographydetermination content once more, by the highly effective liquid phase,infrared, the ultraviolet spectrophotometric method and so on carrieson the attribute Key word: Ginger flavine; Substitution reaction; Crystal;Synthesis.

再次进行合成实验、产物提纯、通过高效液相色谱法测定含量，以高效液相、红外、紫外分光光度法等进行表征关键词：姜黄素；取代反应；晶体；合成。

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

The gasolene, kerosene, and the gas oil fractions are purified to remove unwanted materials, mainly sulphur compounds.

汽油，煤油，和气体油馏分被提纯以去除不想要的物质，主要是硫化物。

Alfred G. Gilman and his co-workers used genetic and biochemical techniques to identify and purify the G protein.

艾佛烈 G。 Gilman 和他的同事用遗传基因的和生物化学的技术识别并且将 G 蛋白提纯。

In this article,we developed a rapid method for quantitative of glutenin micropolymer basedon improved extracting technique.

在改进的蛋白质分离提纯方法的基础上，建立了快速测定谷蛋白大聚合体含量的分析方法。

In this article, we developed a rapid method for quantitative of glutenin macropolymer and soluble glutenin protein largely based on the new 1-propanol extracting technique.

在新的蛋白质丙醇分离提纯方法的基础上，建立了快速测定谷蛋白大聚合体和可溶性谷蛋白聚合体的分析方法。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。