接种环

Involucrata Bailt, younger than 5 -year old, used as explants were inoculated in improved MS medium after ring-barking and 25 treatments for improved MS mediums adding 0,0.1,0.2,0.3,0.4 mg/L NAA, 0,10,20,30,40 mg/L bean sprout juice and 0, 5, 10, 15, 20 mg/L tomato juice respectively were set up to select the optimum medium.

方法采集5年生以下珙桐的嫩枝条幼嫩茎为外植体，环割后接种在改良MS培养基上，培养基设25个处理，分别添加浓度为0、0.1、0.2、0.3、0.4 mg/L的NAA，浓度为0、10、20、30、40 mg/L的豆芽汁和浓度为0、5、10、15、20 mg/L的番茄汁。

It was seen that there were obvious epithelial changes caused by erosion such as cilium shedding, cell stripping and surface depression. In the meantime, there were mycoplasmas adhering to the epithalial surface or tips of remainder cilia.

扫描电镜观察，在接种MG的气管环上，见到明显的纤毛脱落、细胞剥离、粘膜面凹陷等上皮受侵蚀变化，同时见霉形体附着于光秃的上皮表面或残存纤毛的顶端。

These cells were then cultured in RPMI 1640 with 0.3% gelose for 10 to 14 days and the colonies were counted. LoVo cells and A549 cells were also inoculated subcutaneous in BALB/c nude mice.

将裸鼠皮下接种LoVo，A549细胞，72h后分组，每次注射20万IU/kg或100万IU/kg，500万IU/kg IFN-β，每天1次，连续10d，同时设置生理盐水对照组、标准品组和注射用环磷酰胺治疗组。

Place a loopful of the inoculum into the drop of stain and mix with the loop.

加入一菌环量的接种原至一滴染剂中，并用接菌环将之混合。

We did a lot of experiments, such as separating, cultivating and appraising pathogetic epiphyte, studying physiological character of pathogen, doing manual inoculation, filtrating indoor medicament, and cultivating confrontation in petri dish. The result of study showed the pathogen was Armillaria ostoyae..

通过对病原菌的分离培养与鉴定、病原菌生理学特性研究、人工接种试验、室内药剂筛选、平板对峙拮抗培养等试验的研究结果表明，引起该病的病原菌为奥氏蜜环菌Armillaria ostoyaeRomagn。

Female CB6F1 mice (F1 mice in short) which had been hypodermically inoculated with hepatoma cells (H22) were as recipients, and the C57BL/6 mice were as the donors. CB6F1 mice received intraperitoneal chemotherapy with cyclophosphamide. Fresh bone marrow and spleen cells from C57BL/6 mice were as donor cells, which were divided into with or without radiation of 60Co.

实验方法：以皮下接种肝癌细胞（H22）的CB6F1（BABL/C×C57BL/6杂交鼠，简称F1）雌性小鼠为受体鼠，经环磷酰胺腹腔化疗后，取C57BL/6小鼠的新鲜骨髓及脾脏细胞作为供体细胞，供体细胞分为经60Co照射和未经60Co照射。

Methods （1）the experiment in body:the knubbly cell S 180 and the cwer cancer knubbly cell H 22 was inoculated in left and front axillary of rats,which were taken medicine in stomach next day.Saline,cyclophosphamide and different dose Fufangshuizhiwan were used re-spectively in negative comparison group,electropositive comparison group and Chinese medicine group for12days.

（1）体内抑瘤实验：取小白鼠左前腋下接种S 180 瘤种和H 22 肝癌瘤种，次日开始灌胃给药，阴性对照组给予生理盐水，阳性对照组给予环磷酰胺，中药组分别给予大、小剂量的复方水蛭丸，连续用药12天。

Rhesus macaques could be infected by the inoculation of thyrocricoid puncture. And it was proved to be a good method for the similar experiments.

环甲膜穿刺接种恒河猴感染H5N1流感病毒具有很高的可行性，是恒河猴感染H5N1流感病毒和造模的良好途径，并为其它通过呼吸道感染的病原的造模和研究工作提供参考。

We inoculated rhesus macaques by thyrocricoid puncture and observed the clinical signs, pathologic changes and so on in a short period.

利用1株鹅源性H5N1流感病毒经环甲膜穿刺接种恒河猴，通过观察恒河猴在接种后短期内各项指标的变化来评价呼吸道吸入法接种恒河猴感染H5N1流感病毒的可行性。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。