抑制的人

Sulfonamide anilide compound could inhibit human bladder cancer cell growth in vitro, most likely through induction of P21(superscript waf/cif1) mRNA expression and subsequent arrest of cell cycling at G1 phase.

Compound在体外能有效抑制对传统化疗耐药的人膀胱癌细胞生长，其抗肿瘤生长机制可能是通过上调P21(上标 waf/cif1)mRNA水平从而使细胞阻滞于G1期所致。

Our citizens have the right to protection from the incompetency of public employees who hold their places solely as the reward of partisan service, and from the corrupting influence of those who promise and the vicious methods of those who expect such rewards; and those who worthily seek public employment, have the right to insist that merit and competency shall be recognized instead of party subserviency or the surrender of honest political belief.

我们的公民有权利防止那些只因效忠党派而得到职位的公务人员的不称职的行为，有权利抑制那些许诺给人公职的人的腐败行为以及那些期望得到这些职位的人所采取的卑劣手段。那些合格的公务人员应该有权利要求人们承认他们的才华和才能，而不是对党派的愚忠或放弃诚实的政治信仰。

Objective: To investigate the effects and the mechanisms on oxymatrine inhibiting proliferation of HepG2 cells.

目的：探讨氧化苦参碱对人肝癌细胞株(HepG2)增殖抑制的影响及其作用机制。

Thus, the kleptomaniac or the person who is truly already addicted to narcotics does seem to be suffering from something resembling a sickness and, moreover, to be suffering from something which makes it very difficut if not impossible for him to control his actions.

因此，有盗窃癖的人或者那些真的沉溺于毒品中的人真的要承受一些类似疾病使人痛苦的东西，而且如果控制自己的行为并不是不可能的，那么将要承受一些为抑制行为而带来的困难的痛苦。

Many consumers believe that calcium and vitamin D can only choose one product, this is a misunderstanding, due to individual differences in the prevalence of the human body, not all at the same time also the lack of both calcium deficiency of vitamin D, some long-term use of vitamin D it will inhibit the body's own vitamin D formation, or trigger a large number of vitamin D intake and Yun Ji-poisoning, poisoning symptoms of hypercalcemia caused by liver and kidney dysfunction and soft tissue calcification, manifested as loss of appetite, unresponsive, rhythm arrhythmia, difficulty in breathing, nausea and vomiting, polydipsia polyuria, constipation and so on.

许多消费者认为，补钙只能选择与维生素D合一产品，这是一种误解，由于人体个体差异普遍存在，并不是所有的人同时既缺钙又缺维生素D，部分人长期服用维生素D反而会抑制体内自身维生素D的形成，或是引发维生素D大量摄入而积蕴中毒现象，中毒症状为高钙血症引起的肝肾功能损伤和软组织钙化，表现为食欲减退、反应迟钝、心律不齐、呼吸困难、恶心呕吐、烦渴多尿、便秘等。

Put no trust in your own learning nor in the cunning of any man, but rather in the grace of God Who helps the humble and humbles the proud.

不可倚仗你的学问或者别人的狡谋，而要依赖天主的圣宠，祂帮助谦逊的人而抑制骄傲的人。

PDT has a significant killing effect on human pancreatic carcinoma cell lines in vitro, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and light irradiation. The sensitivity of the two cell lines to PDT is different.

光动力作用对体外培养的人胰腺癌细胞具有明确的杀伤效应，其对细胞的相对抑制率具有显著的剂量效应关系；2株人胰腺癌细胞对PDT的敏感性不同。

Flammulina stipe contains a protein that can inhibit asthma, rhinitis, eczema and other allergic diseases, there is no sick people can also eat Flammulina to strengthen the immune system.

金针菇菌柄中含有一种蛋白，可以抑制哮喘、鼻炎、湿疹等过敏性病症，没有患病的人也可以通过吃金针菇来加强免疫系统。

The results showed that (1) the positive staining of ChAT was obviously observed in the cells of the three kinds of human pituitary adenoma, however, it was lower than that in normal human pituitary gland;(2) ACh had a similar effect on the proliferation of the three kinds of human pituitary adenoma cells. ACh at 0.1～10 μmol/L decreased the ［3H］TdR incorporation and the MTT A value in a dose-dependent manner. At the same time, ACh decreased the ratio of S or G2 phase pituitary adenoma cells significantly, but increased the ratio of G1 phase pituitary tumour cells markedly;(3) the effect of acetylcholine on the proliferation of human pituitary adenoma cells was inhibited by atropine, but not by tubocurarine;(4) ACh had no effect on the apoptosis of human pituitary adenoma cells cultured in vitro.

结果发现：(1)三种垂体腺瘤细胞中均有胆碱酯酶的表达，但明显少于正常垂体；(2)ACh对三种类型的人垂体腺瘤细胞增殖代谢的影响相类似，不同浓度的ACh能明显抑制体外培养的三种人垂体腺瘤细胞的增殖，呈明显的剂量效应关系，同时ACh能减少垂体腺瘤细胞进入S、G2期的细胞比例，而使处于G1期的细胞比例增加；(3)ACh的这种作用可被阿托品阻断，但不受筒箭毒的影响；(4)ACh对体外培养的三种人垂体腺瘤细胞凋亡无明显影响。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。