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Oogonium develops into early oocyte in the ovary, and then the oocyte leaves the ovary for the coelomic fluid in the form of single cell or cell mass followed by the rapid separation of the group of oocytes into individual ones. Oocyte enters into the nephridium after its maturation. The rupture of germinal vesicle marks the oocyte maturation. Oocyte in the coelom does not have follicle membrane and vitelline membrane is formed and developed by the oocyte itself. Smaller oocyte (0μm in diameter) is round, and larger ones (≥60μm in diameter) is ovate. The short and long diameters of a morphologically mature oocyte are about 115—120μm and 140—145μm respectively, and the vitelline membrane is 7—9μm thick.

卵原细胞在卵巢中发育至早期卵母细胞时期单个或成团脱离卵巢入体腔液中,卵母细胞团细胞很快分离为单个细胞;卵母细胞在体腔液中发育成熟后进入肾管;生发泡破裂是卵母细胞成熟的标志;体腔中卵母细胞无滤泡膜,卵黄膜的形成与发育靠卵母细胞本身;卵径小于60μm的卵母细胞呈圆形,卵径大于60μm 的卵母细胞为卵圆形,形态上成熟的卵母细胞短径约115—120μm、长径约140—145μm、卵黄膜厚7—9μm。

To evaluate the role of mature cumulus cells from oocyte-cumulus complex in in-vitro maturation and establish a new culture technique which is convenient to carry out.

探讨人类成熟卵丘细胞在未成熟卵母细胞体外成熟培养中的作用,并建立一种简易的实施技术。

Techniques in embryo technology (such as in vitro production of embryos and animal cloning) need large quantities of high quality oocytes. But the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. It is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo.

目前胚胎工程技术研究和开发(如体外生产胚胎和体细胞克隆等)需要大量高质量的成熟卵母细胞,但利用屠宰动物卵巢卵母细胞经过体外成熟培养而获取的卵母细胞质量还远不如体内成熟卵母细胞,其原因一般认为是由于缺乏体内主卵泡阶段的获能作用。

Vg could be detected in haemolmph of the water—fed female at 48h after eclosion, too.

用10%的蜂蜜和清水分别饲喂两组成虫(简称"蜜组"和"水组"),以研究成虫期的营养对Vg发生和卵巢发育的影响,结果显示,成虫期营养对于Vg的启动合成并不起关键的决定性作用,两组成虫均可以在羽化的48小时检测到Vg,且水组中的血淋巴水平并不低于蜜组对照,但绝大多数水组个体的卵巢发育停留在卵黄沉积的某一时期,此时在血淋巴中也检测不到,只有约20%的饲水个体卵巢可以发育成熟,出少量卵;用高剂量的ZR515于羽化当日点滴处理饲水个体,可以使所有接受处理的饲水个体的卵巢发育成熟,但是产卵〓依旧低于对照组的,不能恢复正常;这说明成虫期营养对卵巢中全体卵母细胞的成熟是必需的,其作用的产生是通过内分泌系统途径实现的。

The shorter internodes and out-growth of anxillary buds found in antisense LeETR2 and leaf epinasty observed in antisense LeETRl plants are phenotypes consistent with the negative regulation model of ethylene.

5植株衰老和叶片脱落延迟,花瓣脱落也明显的延迟,在转反义LeETR1基因番茄的果实成熟特性被明显改变,表现在乙烯释放高峰延迟,果实颜色出现变异,以及和成熟相关的酶活性的改变,然而,转反义LeETR2基因的果实的成熟特性与对照相比无明显差别。

Category: D Type: Soft-ripened Milk: Cow Origin: Ireland White mold, soft-ripened cheese made from raw or pasteurized milk in 200-g and 1.7-kg sizes. Robust flavor when ripe. Soft/ creamy to buttery texture with mushroomlike tang unlike other soft-ripened cheeses.

类别:D 类型:半软成熟奶:牛奶原产地:爱尔兰白色模塑、软质成熟,用生牛奶或巴氏灭菌牛奶制成的从200克到1.7千克的奶酪;奶酪成熟后具丰富的口味;软质,从乳脂状到奶油状的质地,与其他软质成熟奶酪不同,此奶酪带有强烈的蘑菇般的味道。

The result showed that different concentration of FSH and LH were needed for sheep oocytes IVM in different season Xinjiang. FSH 12.9ug/ml,LH0.86IU/ml is proper to sheep oocytes IVM in summer-autumn anestrous period. FSH 8.6ug/ml,LH0.57IU/ml is proper to sheep oocytes IVM in autumn oestrous period.

结果表明:新疆绵羊卵母细胞在不同的季节中,体外成熟所需的FSH、LH浓度不同,夏秋乏情季节适宜于体外成熟的激素浓度为FSH 12.9ug/ml,LH0.86IU/ml;秋季发情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml,LH0.57IU/ml;春季乏情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml,LH0.86IU/ml。

Three periods petroleum accumulation exists in South-of-fifth-area oil field, the first period oil and gas is the Jiamuhe group mature and high mature hydrocarbon and the Fengcheng group mature hydrocarbon in late Permian to early Triassic, was destroyed in the Indo-Chinese movement intermediate stage, but some still is conserved in good conserved condition, which is one of exploration objects. The second period is the Fengcheng group high mature hydrocarbon in late Triassic to early Jurassic, which is the important exploration target. The third period is the Jiamuhe group overmature dry gas in the late Jurassic Period to the early Cretaceous.

五区南油气藏存在三期成藏,第一期是佳木河组过成熟干气和风城组成熟油气,成藏期在晚二叠世到早三叠世,在印支运动中期被破坏了,但是在保存条件好的地区仍存在这一期油气藏,它是勘探的对象之一;第二期是风城组高成熟油气,成藏期在晚三叠世到早侏罗世,也是勘探的对象之一;第三期是佳木河组过成熟干气,成藏期在侏罗纪到早白垩世。

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响,以探索猪卵母细胞体外成熟规律,提高体外培养成熟度,为ROSI技术体系准备优质卵母细胞;然后针对猪圆形精细胞的体外分离进行了试验,进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection,ICSI)操作的比较研究了影响ROSI操作的因素。

The meiosis of oocytes differ from mitoses of general cells. Especially in most mammalian species, oocytes are formed during fetal life, but they are arrested at the prophase stage of the first meiotic division until around the time of ovulation.

卵母细胞的成熟分裂有别于一般细胞的有丝分裂,尤其是哺乳动物,在胎儿期或出生后不久,初级卵母细胞就陆续进入第一次成熟分裂前期阶段,但长时间的&休止&于此期,直到性成熟时在促性腺激素等因素的作用下,第一次成熟分裂才重新启动。

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