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异构酶

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Specific activity of NADP+??isocitrate dehydrogenase, methylmalonyl ??coenzyme A mutase and the concentration of α??

通过实验测定发现,发酵过程补加豆油后,异柠檬酸脱氢酶、甲基丙二酰CoA异构酶的比活以及α?

Nine seasickness adaptive proteins were identified by PMF: peroxiredoxinⅠ, peroxiredoxinⅡ, light molecular-weight neurofilament, ubiquitin carboxyl-terminal hydrolase PGP9.5, and glutamine synthetase were highly expressed; carbonic anhydraseⅡ, triosephosphate isomeraseⅠ, phosphoglycerate mutase isozyme B and mitochondrial voltage dependent anion channel were lowly expressed.

获得了9个晕船适应相关蛋白质:硫氧还蛋白过氧化物酶Ⅰ、硫氧还蛋白过氧化物酶Ⅱ、低分子量神经丝蛋白、泛素羧基末端水解酶PGP9.5和谷氨酰胺合成酶上调;碳酸酐酶Ⅱ、磷酸丙糖异构酶Ⅰ、磷酸甘油酸变位酶B和线粒体电压依赖型阴离子通道下调。

L-arabinose isomerase can isomerize L-arabinose and D-galactose into L-ribulose and D-tagatose, respectively, which is currently the most effective biological catalyst for D-tagatose production.

L-阿拉伯糖异构酶能分别催化L-阿拉伯糖和D-半乳糖异构为L-核酮糖和D-塔格糖,它是目前生物法生产新型功能性因子D-塔格糖最为有效的酶。

Nevertheless, no homologues of typical molecular chaperones have so far been found in periplasm, and the recently reported chaperone activities of periplasmic protein disulfide isomerase and peptidyl pro-lyl isomerase seem to be too weak to satisfy such assumed needs.

虽然膜间质中的蛋白二硫键异构酶和脯氨酸顺反异构酶最近被报导具有分子伴侣活性,但是这种活性的检测使用的不是天然的膜间质蛋白,而且没有捕获到复合物的形成,因此看起来仍不能够满足前面分析的对膜间质蛋白保护的需求。

To obtain thermostable xylose isomerase, the gene xylA from an extremely thermophilic bacterium,Thermus thermophilus HB8 was cloned and its product was overexpressed in Escherichia coli BL21(DE3) with expression vector pET22b.

为获得具有高热稳定性的木糖异构酶,运用基因工程技术,从嗜热栖热菌Thermus thermophilus HB8中克隆到嗜热木糖异构酶基因xylA。

We also strongly suggest that the recently reported chaperone acitivity of periplasmic PDI-ase and PPIase assayed by traditional substrate should be reexamined using periplasmic proteins as substrate due to the significant difference between those two classes of proteins, the possible chaperone fuctions of PDIase and PPIase in vivo should be reappraised.

同时,由于本实验结果显示膜间质蛋白与常用的分子伴侣测活底物蛋白在聚集行为上存在极显著的区别,我们建议对最近报导的膜间质蛋白二硫键异构酶和脯氨酸顺反异构酶分子伴侣活性以膜间质蛋白为底物再进行验证,对其在膜间质是否发挥分子伴侣功能重新慎重考虑。

In our results, we found four proteins have isoforms; these proteins are peptidyl-prolyl cis-trans isomerase A, prohibitin, enolase 1, myeloperoxidase precursor.

同时,我们发现肽基辅胺酸顺反异构酶(peptidyl-prolyl cis-trans isomerase A)、抑制素、烯醇酶(enolase 1)、过氧化骨髓酶有异构体的产生。

By using lysozyme, RNase A and protein disulfide isomerase as model proteins, we present a hypothesis that protein cross-linking can be accomplished in three concerted steps:(1) a change in protein conformation;(2) formation of interchain disulfide bonds; and (3) formation of interchain isopeptide cross-links.

我们以溶菌酶、核糖核酸酶A和蛋白质二硫键异构酶为模型对异肽键进行了体外详细的研究,提出了解释异肽键形成机理的假说:1蛋白质发生构象变化包括二级结构的改变;2蛋白质形成分子间二硫键:3二硫键的形成帮助蛋白质形成了分子间异肽键。

He analysis of the category and function of TCB stress inducible proteins showed that different groups of proteins were induced by 5 mg L^(-1) TCB stress. They are detoxification enzymes (including esterase, aldo/keto reductases, and glutathione S-transferase), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1), phytohormone metabolism and regulation related enzymes or proteins (including aci-reductone dioxygenase 4, beta-glucosidase, two members of pathogenesis-related proteins from family 10), primary and secondary metabolism regulative enzymes (including translational elongation factor Tu, cytosolic orthophosphate dikinase, triosephosphate isomerases, alanine aminotransferase, and isoflavone reductase).

CB胁迫能诱导根系内不同类型蛋白质的表达,它们是:解毒酶(包括脂酶、醛/酮还原酶、谷胱甘肽-硫转移酶),细胞壁物质代谢相关酶(包括蛋白转葡萄糖基酶、GDP-甘露糖-3,5-异构酶1),激素代谢或调节相关酶(包括酸式-还原酮二加氧酶、β-葡萄糖苷酶、病程相关蛋白家族10中的2个不同蛋白),原初或次生代谢相关酶(包括转录延长因子、细胞质磷酸丙酮酸双激酶、磷酸丙糖异构酶、丙氨酸氨基转移酶和异黄酮还原酶)。

To determined the role of this gene in gluconeogenesis and in pathogenesis of Xcc, mutants of pgi gene were constructed by homology plasmid integration method.

六磷酸葡萄糖异构酶是糖异生途径中另一个关键酶,在野油菜黄单胞菌中编码该酶的基因是pgi基因(Xcc 8004基因组中基因编号为XC2379)。

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