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To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞,流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度,Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

Effect of solute adsorption on vapor pressure osmometry measurment was studied by four low molecular weight organic compounds and five narrow MWD oliomeric polystyrene samples with known average molecular weight in Knauer VPO apparatus.

在气相渗透仪上,用四个低分子量有机化合物和五个已知平均分子量的窄分布聚苯乙烯低聚体,对VPO方法中的溶质吸附效应作了研究,提出了一种对吸附效应作改正的方法。

By means of our own constant head permeameter,we have measured the permeability coefficients of soil-rock-mixture s with different gravel content,and the results show that there exists an index relationship between them.

通过自制的常水头渗透仪,测定了不同含砾量时土石混合体渗透系数值,研究发现含砾量与土石混合体渗透系数之间存在指数关系;基于幂平均法,提出了土石混合体复合渗透系数的计算公式,并通过试验结果验证了该式的正确性,为土石混合体渗透系数的理论计算提供了一个简明有用的计算工具

The mean velocity distributions in turbulent boundary layers of smooth surface and two different riblet surfaces are measured with IFA300 constant-temperature anemometer in low-speed wind tunnel to validate the full development of turbulent boundary laye. Friction velocity and wall friction stress of turbulent boundary layer are obtained with the least-squares fitted curve of the sublayer velocity distribution based on Spalding formula, and virtual origin position of riblet surface can be acquired accurately.

在低速风洞中来流速度一定的情况下使用IFA300恒温热线风速仪测量了光滑表面和两种不同尺寸的脊状表面湍流边界层平均速度分布剖面,并验证了试验段湍流发展的充分性;通过应用Spalding壁面公式使用最小二乘法精准拟合了实验测量的边界层内层速度分布曲线,得到了湍流边界层壁面摩擦速度并进一步求得湍流壁面摩擦应力,较准确地计算出脊状表面的虚拟原点位置,并通过与对数律公式拟合结果比较分析,证实了该方法更加准确有效。

No Adoption was allowed to take place without due provision for the sacra of the family from which the adoptive son was transferred, and no Testament was allowed to distribute an Inheritance without a strict apportionment of the expenses of these ceremonies among the different co-heirs.

如果对养子原来的家族不举行适当的家祭,则不能进行"收养":如果祭仪的费用不在各个共同继承人中严格地平均分摊,则不准根据"遗命"来分配一个"继承权"。

Results: Among the patients who had normal joint laxity, the mean side-to-side difference in anterior tibial translation on testing with the KT-2000 arthrometer at a minimum of twenty-four months postoperatively was 2.7 ± 1.9 mm in the bone-patellar tendon-bone graft group and 3.5 ± 1.7 mm in the hamstring graft group. This difference was not significant.

结果:关节松弛度正常病人术后24月利用KT-2000 型关节测量仪检查平均双侧胫骨前方移动度差别,髌骨-骨-腱移植组为2.7 ± 1.9 mm ,筋膜移植组为3.5 ± 1.7 mm ,差别无统计学意义。

Coronary flow velocity was recorded by using Cardiometric FloMap Ⅱ by introducing the Dopplerflowire into the target arteries. The average peak veloity and coronary flow reserve were measured before anafter PTCA and after stent implantation.

在PT-CA前后和支架植入术后,采用Cardiometric FloMap Ⅱ血管腔内多普勒血流速度描记仪和多普勒导丝,测量狭窄近端和远端的平均峰值流速、舒张期与收缩期流速比、近远端血流速度之比、冠脉血流储备。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Four parameters of platelet were detected (using Japan East Asia Sysmex F-800 hematocyte automatic counting machine) and compared.

采用日本东亚SysmexF-800型血细胞自动计数仪检测了150例肝炎后肝硬化、150例重型肝炎、114例急性黄疸型肝炎、107例慢性活动型肝炎和50例健康成人的血小板功能4项指标:血小板计数、平均血小板体积、血小板压积和血小板分布宽度。

Keratometer was used to check the corneal curve at 2 direction for 3 times to offer the mean K value, and a nearest basal lens to K value was selected as the trial lens.

步骤一:用角膜曲率仪测量角膜曲率,两个方向各测三次,取平均值,得到角膜曲率的平均K值,选取与K值接近的镜片作为试戴片。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严,以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆,把帆卷起,系住。

Therefore, positively advances the interest rate marketability reform is one of current our country finance reform important tasks.

因此,积极推进利率市场化改革是当前我国金融改革的重要任务之一。