干谷

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

Next day, if there was no thunder shower, all hands would help rake and pitch and load, and the hay would be hauled to barn in the high hay wagon, with Fern and Avery riding at the top of the load.

如果运气好没有下雨的话，所有人都会帮忙把草扎起来，摞成一堆，等干了以后就会放在谷仓里堆成高高的一堆，而弗恩和艾弗利就会坐在高高的顶端玩耍。

We dry herbs by stringing them from rafters in the barn.

我们把草药挂在谷仓的椽上使之变干。

One day in 1867, a farm hand from the village of Lapcourt, who was somewhat simple-minded, employed here then there, depending on the season, living hand-to-mouth from a little charity or in exchange for the worst sort of labor, sleeping in barns and stables, was turned in to the authorities.

1867的一天，一位头脑简单，一年四季在不同地方打工，靠一点施舍和干苦活填饱肚皮、夜里则住在谷仓或马厩里的农工被人告发。

With the development of highway construction, the speed of cars has been gradually increased.

随着公路的发展，汽车的车速也在不断提高。

So we basically had to develop a bond with the animal in terms of socializing with it.

所以我们就主要想通过跟它沟通的方式来建立一种良好的关系。