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Positive rate was relatively high, and the foreign gene could also maintain in testicular tissue for a long time, it could still be detected in the tissue in 7 months after being injected. However. Deferent results could be gained when the detection were made with different primers on different sites of the recombinant plasmid. This could be explained by transgene lost when the foreign gene recombinated and integrated in heterogenetic cell, or transgene degradated by cell nucleases.

然而,我们也看到,在后代检测中,利用不同的引物扩增重组质粒不同部位时,检测结果存在差异,转基因阳性率不同,这种差异说明当外源基因进入到异源细胞内,在重组整合时发生了外源基因丢失的现象,并由此导致外源基因的整合率下降,也可能是受细胞内核酸酶的作用,使得外源基因遭到降解。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示,重组疫苗接种1周后,免疫鸡产生抗IBV的抗体;而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组;攻毒后,异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势,并且该组低水平CD4+的状态一直持续到试验结束,而其他组CD4+ T淋巴细胞均迅速上升,峰值达到14.5 %;同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达,而其他组攻毒后均无明显变化;保护率结果显示,同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %,与其他各组相比均有显著差异;另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻,其排毒时间和排毒量也均有所减少;强毒攻毒后所有试验组体重无显著差异

In some sites,plantations were completely replaced by local weeds .And thus a new landscape developed with the function of preserving soil and slope and beautifying the scene,such asSetaria viridis and Imperata cylindraca var. major .

4不同的人工种植植物品种在高速公路环境下的存活率是具有显著性差异的:大叶樟和小叶女贞相对于迎春具有显著性差异,大叶樟与小叶女贞之间则差异性不显著。

And fall in difference of ego monitoring rate, the existence of the impressional management that the individual place in the enterprise takes has difference, and the sense of the person that accept is endless and same.

而在自我监控程度差异下,企业中的个体所采取的印象管理的存在有差异,且接受者的感觉不尽相同。

Results The result shows that between male and female, the difference of thickness of each layer is inapparent. But the difference of quantity of mitral cell is apparent.

结果 研究表明:雌雄大鼠之间嗅球内部各层结构差异不显著,但是僧帽细胞数量差异极显著

The results showed that(1)even though there were no significant gender differences increative thinking,neither in Chinese children nor in Germanchildren,and neither in supernormal children nor in normalchildren,the males were superior to the females in mentalfolding;( 2) there were significant gender differences inlearning motivation and technical problem comprehension andactivities in science.

具体表现为:(1)在数字、图形和实用创造性思维方面,在中国和德国儿童中都没有发现创造性思维方面的性别差异;(2 )在心理折叠、学习爱好、技术问题理解和科学活动方面,在中国和德国的超常儿童和常态儿童中都有不同程度的性别差异存在。

The results showed that there were differences between adaption and regulation of different wheat varieties by prophyl dihydro jasmonate, so as to show diversity of gas exchange parameters, such as photosynthesis, water usage, etc. There was no evident difference between the reaction of two spring wheats (Jinchun1 and 1608) and the winter wheat (Xiaoyan 22) to PDJ. Different parameters of different wheat varieties had inequable reaction to different concentration of PDJ. Photosynthesis rate, water use efficiency and stomatal limiting value were enhanced when PDJ concentration reached a high degree. At the same time, leaf transpiration rate and stomatal conductance decreased. Therefore, wheat could maintain normal physiological activities while environmental conditions were changed.

结果发现,面对类似激素双氢茉莉酸丙酯调控下的不同品种小麦适应与调节存在差异,从而导致光合和水分利用等气体交换参数的表现也存在差异;两种春小麦(金春1号、l608)和一种冬小麦(小偃22)之间对PDJ的反应没有明显的冬春性间的差别;对不同品种、不同参数,PDJ起到作用的浓度不同,但无论如何PDJ都有相同的作用,当这种激素达到一定的浓度后,就会增强小麦的光合速率和水分利用率,增加气孔限制值,降低叶片的蒸腾速率和气孔导度,从而在外界条件变化时保持体内水分、延缓气孔关闭,维持体内正常的生理活动。

The sequence difference of cytochrome b gene between Lipotes vexillifer and Inia geoffrensis is 17.18%(56/326bp), and between L.

白暨豚与亚河豚间的序列差异为17.18%(56/326碱基),与江豚之间的差异为15.03%(49/326碱基)。

The disparity in each trait were studied by variance analysis. More detail analysis were made for all traits and cuts by multi-statistic analysis, the results were shown as follows:Among all measured meat traits, crude fat, crude protein, tenderness, water holding capacity, pH value, meat colour, area, diameter and density of muscle fibre, amount of muscle fibre in fascicle, inosine, inosinic acid imp, and dissociative glutamine were significantly different between sex.

对数据统计分析结果表明:在所有肉质指标中,除水分、粗灰分和硫胺素在三种性别间彼此无显著差异外,粗脂肪、粗蛋白、嫩度、系水力、pH值、肉色、肌纤维面积、肌纤维直径、肌纤维密度、肌束内肌纤维根数、肌苷、肌苷酸和谷氨酸钠在不同性别秦川牛之间差异均显著(P<0.05)。

These results indicated the differences between interfamily and intraspecific pregnancy.

科间妊娠和同种妊娠的免疫反应有差异,这些差异可能是造成科间妊娠失败的原因之一。

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Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达,对卵巢癌细胞生物学特性的影响。

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弯曲:这种病是最常见的原因查明腹泻病,在世界上。

Gangs fill the daily lives of many of our poorest and most vulnerable citizens with a terror that the court does not give sufficient consideration, often relegating them to the status of prisoners in their own homes.

法院没有充分的考虑到黑社会的猖獗所带来的恐怖,这使得我们最穷、最可怜的市民每天生活在他们的阴影当中,成为在家的囚犯。