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After the cells were treated by high concentration toxin for 4d, the content of nitrate-nitrogen in cells were lower markedly than the contrast, after the cells were treated by middle and low concentration toxin for 7d, the content of nitrate-nitrogen in cells were lowered. 3 groups of toxin treating all lowered the total phosphorus content. At the end of test, the phosphorus contently of low, middle, high concentration treating groups were 74.98%, 76.47% and 84.00% of the contrast respectively. The ACP activity of 3 treating groups compared with the contrast appeared the tendency of first lowering then raising.

高浓度毒素处理细胞4d后,细胞内硝态氮含量显著低于对照;中、低浓度毒素处理细胞7d后降低了胞内硝态氮含量。3组毒素处理均降低了胞内总磷含量,到实验结束时,低、中、高浓度处理组的胞内磷含量分别为对照的74.98%、76.47%和84.00%。3组处理组ACP活力与对照相比呈现先降低后升高的趋势。

Lastly,she bring forword a ragged contrapose writing technique that have methodology meaning and apocalypse.

四是参差对照的写法:她提出一种&参差的对照&的写法,认为这样写是更真实的。她所提出的&参差的对照&有方法论的意义和启示。

Species richness and Pielou evenness index were used to measure species diversity. Species richness in polluted area was 32-67, while in control area it was 47-82. Pielou evenness index of tree layer was 0.1161-0.8509, while those of shrub layer and herb layer were 0.1763-0.3249 and 0.0443-0.2601, respectively. The results showed that both species richness and Pielou evenness index were influenced by the point source pollution. Species importance values showed significant differences between polluted area and control area. Dominance of different species had changed, and some species even disappeared under the pressure of pollution, indicating a process of species replacement in plant communities. Such species replacement can provide a scientific basis for species selection for restoration of human-damaged ecosystem. The natural regeneration of Quercus variabilis was insensitive to pollution, while Cupressus funebris was effected seriously.

仅在污染区中出现的物种数量远低于仅在对照区中出现的物种数量;(2)可分出4种基本的群落类型:马尾松林、柏木林、栓皮栎林和马尾松+栓皮栎林,各群落特定层次的物种丰富度指数以及Pielou均匀度指数在污染区与对照区之间存在显著差异;(3)污染胁迫下,物种在群落中的生态优势度发生了变化,反映了群落中不同物种相互替代的过程,这种物种的替代可为今后受损生态系统人为恢复过程中物种的筛选提供一定的理论参考;(4)对群落中王要物种更新的调查发现:栓皮栎林对污染不敏感;柏木林污染区与对照区幼苗数量及分布格局均有明显差异,污染条件下群落未来演替受到严重干扰。

In the natural improved grassland with uncultured and resowed,the sorts of grass increased 5~8 varieties,and the height of elymus dahuricus and the sedge respectively was higher than that of the control area for...

在免耕补播改良天然草地方面,草地植被种类增加5~8种,披碱草高度较对照区高出18cm,苔草高出4cm,其总盖度较对照区高出近20%,而施肥试验草层高度较对照区增加了1.5cm,其盖度由55%增加至90%,增幅为35%。

Inflammatory cells in the hematoma expressed bFGF and proliferating periosteal cells, endosteal cells, medullary mesenchymal cells expressed TGF-β at 3 days postoperatively.

3种生长因子在实验侧和对照侧的含量有明显差别。实验侧总体含量明显高于对照侧(P.01),各个时间组实验侧含量也高于对照侧。

Along with seawater stress strength upward, the ratio of root to shoot, Na+, Cl- and soluble sugar contents were increased significantly. Under the 10% and 20% seawater treatments, chlorophyll contents have no obvious difference compared with the control, but significantly decreased when the seawater stress further increased. Under the 10% seawater treatments, K+ contents in the shoot and root of Lactuca indica seedlings have no distinct difference compared with the control, but along with seawater stress strength upward, K+ content were markedly decreased by degrees. However, soluble protein content first increased then decreased with seawater concentration augment.

结果表明:苦荬菜幼苗地上部受海水胁迫较为显著,而根在30%海水以内与对照相比没有显著差异;根冠比随着海水比例的增加而提高;10%和20%海水处理下,叶绿素含量与对照相比差异不显著,但随着海水浓度的进一步增加,叶绿素含量显著下降;10%海水处理下,苦荬菜地上部分及根K+含量与对照差异不显著,高于10%海水处理,K+含量随海水比例的增加地上部和根部均逐渐显著降低;海水处理下,体内Na+和Cl-含量逐渐显著增加;地上部可溶性糖逐渐显著增加,而可溶性蛋白呈先升后降。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下:1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草,获得三个单基因和一个双基因的RNAi沉默表达植株,快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加;叶组织内Na+离子含量和K+离子含量均略高,但差异不显著;K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降,但差异不显著;K+离子含量比对照显著降低。

Result In 5 kinds of chemical treatments, treating Z. macrostachya seeds with 20 % NaOH solution for 20 min and then cleaning the seeds with clear water got the best effect of breaking seed dormancy. By this treatment, the seed germination potential reached 83.5% in 10 d, being 58.2 % higher than CK; reached 85 % in 15 d, being 55 % higher than CK, which was near to the potential germination rate of Z. macrostachya seeds. Compared with CK, the germination duration was obviously shortened.

结果]在5种化学处理中,用浓度20%NaOH溶液处理大穗结缕草种子25min,再用清水清洗,对解除种子休眠效果最佳,10d发芽势达83.5%,比对照高出58.2百分点,15d发芽率达85%,比对照高出55.0百分点,已接近大穗结缕草种子的潜在发芽率,发芽历时比对照明显缩短。

The results were summarized as follows:① The length and dried weight of shoot and the differentiation of leaves were enhanced on a culture medium with α-Naphthaleneacetic acid, and the effectiveness of NAA became stronger with the incerase in concentration within a range of 0.001 mg/L to 0.1 mg/L.

结果表明:①萘乙酸促进银杏茎尖伸长生长和叶片分化,随NAA浓度增加其作用增强,在NAA为0.1 mg/L时茎尖长度为对照的1.47倍,分化叶数为对照的2.62倍,茎尖干重为对照的6.45倍。

Results:The levels of CD4+T cells in active and remissive SLE were significant lower than normal controls; the positive rate of CD4+CD25+T cells in both active and remissive SLE was higher than that in normal controls; the levels of IL10 in active stage of SLE were significant higher than in remissive stage of SLE or in normal controls.

结果:活动性和非活动性SLE 患者CD4+T细胞总数均低于正常对照者;活动性和非活动性SLE患者CD4+CD25+T细胞阳性率高于正常对照者;活动性SLE患者IL10浓度显著高于非活动性SLE患者和正常对照者。

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