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Mr Christiansen's heirs decided to stand by the family business.

家族成员们决定维护家族的生意。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子,在许多同种的真核生物有它的同源基因,这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关,而这个家族的蛋白质和DNA的结合能,各种ARID蛋白质的专一性尽相同,过大致上偏好於和AT-rich的序结合;我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因,分别是garid1和garid2,我们首先对於garid1做分析;将AU1标记接到gARID1转染形鞭毛虫,用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下,过其阳性染色和蛋白质表现有明显增加;EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合,经由突变序分析,也显示gARID1的结合序为AGATC和AATAAAATA,随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The next generation of his family has a heritor,so he has no stress under the obligation for carrying on the family line.

他所在家族已经有男孩继承了,所以在家族的传宗接代问题,他不用费神了。

So after the duke had acquired Romagna and beaten down the Colonna, two things prevented him from maintaining that and going further ahead: one, that his arms did not appear to him to be faithful; the other, the will of France: that is, the Orsini arms of which he had availed himself, might fail under him, and not only prevent him from acquiring but also take away what he had acquired; and the king might also do the same to him.

其一, 他的军队似乎并不忠于他;第二重障碍便是法国的意愿。这就是说,他所征借的奥尔西尼家族的军队很可能会反水,不仅会阻止他去攻城掠地,而且还会将他所占的地盘抢走;法王也会对他做出同样的事情来。他在攻占了法恩扎之后,便向波洛格纳发动袭击,他发现奥尔西尼家族对这次袭击态度冷漠,由此便检验出了彼家族对他的成见。至于法国国王,瓦伦蒂诺公爵也算看穿了他的心思。

Along with some other Medici rivals-including an archbishop-the Pazzi family decided to murder Giuliano and Lorenzo in church.

和一些别的梅地奇家族的对手,包括一位大主教联合,帕齐家族决定在教堂谋杀朱利亚诺和洛伦左。

Like other signore families they dominated their city's government. They were able to bring Florence under their family's power, allowing for an environment where art and humanism could flourish.

如同其他贵族政治家族一样,他们掌握了市政府政权,把佛罗伦萨控制在他们家族的势力范围内,使得艺术和人文主义氛围能够得到繁荣。

BRSK2 is a serine/threonine protein kinase, belonging to the AMPK subfamily of CAMK family.

BRSK2是一个丝氨酸/苏氨酸蛋白激酶,属于CAMK家族的AMPK亚家族。

He spends 4 Influence Points on Maester Horton, the house maester, and 4 Points on Ser Victor Fossoway, the house master-at-arms.

他把4点影响力放在家族的学士——豪登学士身上,另4点放在家族武官——维克托。法瑟维爵士身上。

Henry Tudor, descendant of Duke of Lancaster won victory at Bosworth Field in 1485 and put ht country under the rule of the Tudors. From these Wars, English feudalism received its death blow.

玫瑰战争是指,从 1455 年到 1485 年,以红玫瑰为象征的兰开斯特大家族和以白玫瑰为象征的约克家族之间战争的普遍接受的名称。1485 年,兰开斯特家族的后代亨利都铎取得了博斯沃恩战役的胜利,建立了都铎王朝。

The name Wars of the Roses was refer to the battles between the House of Lancaster, symbolized by the read rose, and that of York, symbolized by the white, from 1455 to 1485. Henry Tudor, descendant of Duke of Lancaster won victory at Bosworth Fireld in 1485 and put the country under the rule of the Tudors.

玫瑰战争是指,从 1455 年到 1485 年,以红玫瑰为象征的兰开斯特大家族和以白玫瑰为象征的约克家族之间战争的普遍接受的名称。1485 年,兰开斯特家族的后代亨利都铎取得了博斯沃恩战役的胜利,建立了都铎王朝。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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