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Furtherly, crosses were made among fertile isolates from rice plants in Jiangsu. Only 6 of 25 crosses produced perithecia and asci, but no ascospore produced. This suggest that the frequency of sexual stage occurrence of Magnaporthe grisea was very low in the rice field in Jiangsu.

用江苏省稻瘟病菌的可育菌株进行互交,25个组合中只有6个组合能产生子囊壳和子囊,但均不产生子囊孢子,提示江苏省稻瘟病菌在田间产生健康有性后代的几率不大。

The hymenium may be directly exposed to the air, as in the gills of the mushroom or may open into a flask-shaped cavity, as in the perithecia of certain ascomycetes.

子实层可能直接暴露于空气,像蘑菇的菌褶,也可能似瓶状开口,如某些子囊菌的子囊壳。

Each perithecium has a round base of diameter 130-200 microns with a neck up to 800 microns long.

每个子囊壳的圆形基部直径130-200微米,颈长达800微米。

Ascospores are small, hyaline, and hat-shaped, 4.5-8 microns long by 2.5-5.5 microns wide, andthey accumulate in a sticky matrix at the end of the perithecium neck where they appear as a cream to pink ball or coil.

子囊孢子小,无色,帽形, 4.5-8微米长,2.5-5.5微米宽,他们累积在子囊壳颈口粘性基质中,在那里他们看上去象奶油色到粉红色的球或卷圈。

Results:(1) The conchosporangia of brown yellow mutant was smaller and mature time was 15 days later than the parents. Thallus grew slowly in early stage, but average daily growth rate reached (7.50±1.18) cm after blade length was over 60 cm.(2)The emerald conchocelis were easy to mature and had special developmental mode that the spherulocytes can directly develop into sporangial branchlets and conchosporangia without thick conchocelis stage. Emerald blades were low in RPE with just (6.4710±0.0184) mg/g dry mass. The thallus grew quickly, average daily growth rate reached (11.95±2.33) cm after blade length was over 60 cm.(3) The conchocelis filaments of breen mutant were thin and short. Thallus grew slowly and had low contents of three phycobiliprotenin and chlorophyl.

实验进行50d。结果:(1)褐黄色突变体藻蓝蛋白和别藻蓝蛋白含量低;孢子囊枝的细胞较小,且大量形成时间比亲本晚15d;幼苗培养初期日平均生长量仅为(1.22±0.28)cm,当叶片长到60cm左右时生长优势逐步凸显,日平均生长量可达(7.50±1.18)cm;(2)翠绿色丝状体容易成熟,发育方式特殊,营养藻丝不经过藻丝加粗阶段,直接由球形细胞发育成孢子囊枝和壳孢子囊;翠绿色叶状体藻红蛋白含量低,仅有(5.5130±1.0496)mg/g;叶状体生长快速,60cm长的藻体日平均生长量高达(11.95±2.33)cm;(3)褐绿色突变体藻蓝蛋白、别藻蓝蛋白和藻红蛋白这3种色素蛋白和叶绿素的含量均较低;藻丝细胞短且细,叶状体生长速度较慢。

Nectria-like fungi have bright-colored perithecia, emcompassing more than 600 species worldwide.

赤壳菌类真菌是一群具有鲜艳子囊壳的真菌,全世界种数超过600种。

18 Days after perithecia appearance was the best time to isolate ascospores, although there were differences between crosses. The highest survival percentage of ascospores could reach up to 85. 5% during this period.

观察发现,虽然不同杂交组合间有一定差异,分离稻瘟病菌子囊孢子的适宜时期,一般在子囊壳开始出现后7~18天之间;在此期间分离出的子囊孢子成活率最高可达85.5%。

When they were cultured with KA3(MAT1-1), KA9(MAT1-2) or Z46(MAT1-2),the numbers of strains which can form perithecia are 22,6 and 4, respectively.

研究结果表明:35个双交配型菌株自交都没有形成子囊壳,分别与KA3(MAT1-1)、KA9(MAT1-2)和Z46(MAT1-2)杂交形成子囊壳的菌株数为22,6和4。

Two isolates ( BO1218 BO1199)can form perithecia when they were cultured with KA3(MAT1-1) and Z46(MAT1-2).Three isolates ( BO1218 BO1199 and BO1535) can form perithecia when they were cultured with KA3(MAT1-1) and KA9(MAT1-2).These five isolates have MAT1-1 and MAT1-2 which are functional.

其中2个菌株(BO1218、BO1199)与KA3和Z46杂交都形成子囊壳,3个菌株(BO1643、BO1563和BO1535)与KA3和KA9杂交都形成子囊壳,表明这5个菌株中存在MAT1-1和MAT1-2,并且具有完整的功能。

It could accelerate the production of ascomata and shorten the maturing time when cellulose added, without conspicuous affect on the structures of ascospores.

实验表明,生长过程中加入纤维素能提高毛壳菌子囊果的产果量并缩短子囊果的成熟所需时间,但对子囊孢子形态特征无明显影响。

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