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In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低,可能是由于其分子量较小,所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中,利用硫氧还蛋白融合方式对该抗原进行修饰,并在大肠杆菌中表达融合蛋白(Trx-B_2),纯化该蛋白后免疫小鼠及家兔,结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后,得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度,并从免疫后的兔血清中提取IgG包被酶联板,利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清,结果阳性率分别为87.5%和43.75%,符合率为71.88%。

Aiming at the two structural features of axle shaft sleeve on the basis of analyzing various forming technologies applied in domestic and overseas in recent years,two new hot extrusion forming technology of axle shaft sleeve are worked out.

针对半轴套管的两种结构特点,在分析了国内外近年来所采用的各种成形工艺的基础上,分别开发了用镦粗-反挤压和扩径-正挤压两道复合工序相结合成形分体式半轴套管和用两次复合镦挤的方法成形整体式半轴套管的两种热挤压成形新工艺,通过物理模拟实验证明了成形工艺的可行性、实用性。

Preparation of quasi-interpenetrating network/functionalized gold nanoparticle composite matrix and its performance for DNA sequencingA new matrix additive,PDMA-functionalized gold nanoparticle, was prepared by "grafting-to" approach to avoid GNP aggregation at high concentration of salt in buffer solution,and then incorporated into quasi-IPN composed of LPA(3.3 MDa) and PDMA to form novel polymer/metal composite sieving matrix(quasi-IPN/GNP-PDMA) for DNA sequencing by CE.

准互穿聚合物网络/官能化的金纳米粒子复合介质的制备及其用于DNA测序性能的研究为了解决GNPs在高盐浓度下易附聚的问题,由&grafting-to&法制备了一种新型介质添加剂,即PDMA官能化的GNPs,并将其加入到由LPA(3.3 MDa)和PDMA所组成的quasi-IPN中得到了用于毛细管电泳DNA测序的聚合物/金属复合筛分介质(quasi-IPN/GNP-PDMA)。

In this thesis, with regard to the stability and deformation of the strut frame made of composed soil nails in soft clay foundation pit, the studies listed below are carried out, considering current research and design status of the soil nailed retaining structure. 1、This paper begins with the concept of the strut frame made of composed soil nails, analyzing the composition and the technique of the construction of the strut frame made of composed soil nails.

本文鉴于软粘土地区深基坑工程土钉支护结构的研究及设计现状,在前人研究的基础上,就复合土钉支护软粘土基坑的稳定性和变形展开相应的专题研究,主要完成了以下几个方面的工作: 1、从复合土钉支护结构的概念入手,对其结构组成、构造形式以及施工方法进行了综合分析。

In order to determine the bacterial composition of the community,11 isolate strains were detected by plate isolation,while a community reset by the 11 isolate strains lost the capacity of degrading cellulose. The capacity of degrading of the filter paper in double deck plate and monolayer plate were determined,only the filter paper in double deck plate were degraded,that means the main or key microbe are anaerobic.

为系统了解复合系的菌种组成,在有氧条件下,利用传统的平板画线法分离到11株单菌,将11株单菌按体积比1∶1重新组合并不具备分解纤维素的能力,利用单层和双层平板滤纸法检测NSC-7的分解能力,发现只有双层平板上的滤纸变黄且降解,说明复合系内纤维素降解的关键菌是厌氧或微好氧菌。

In order to describe the composite value specifically in academic way for our management practice, the thesis offers a quantitative way which measuring sociall value with "relative stable watching ratio" and another ratio of the sum of TV Ads income, watching fees and the profit from the sales of content products to the total potential payment ability of target audients for economic value.

具体来讲,娱乐产品层次的增值管理,首先在于确立合理的价值分类标准,以便有效确定特定内容产品复合价值所对应的细分目标市场,进而通过复合价值的对比分析,切实促进内容产品的社会与经济价值的增长。

The different foaming performance of sodium laureth sulfate, cocamidopropyl betaine and their compounds under different active concentrations, water hardnesses and temperatures were investigated. The results show that the foaming performance of SLES doesn't change under different water hardness, but that of CAB changes with different concentration, and the compounds of SLES and CAB have much better foaming performance and synergetic effect under different conditions.

通过实验主要考察了在不同活性物浓度,不同水硬度条件和不同体系温度下,SLES、CAB和它们的复合物的泡沫性能,结果表明,SLES的泡沫性能受硬水影响不大,CAB的泡沫性能随浓度的变化而有所不同,SLES和CAB的复合物有更优秀的泡沫性能,表现出良好的协同增效性。

The results showed that the POV of hemp seed oil was affected remarkably by temperature and time, and the effect of temperature was greater than that of time. The TBHQ had good antioxidation ability to hemp seed oil. TBHQ or BHA with isoascorbic acid or critic acid exhibited great antioxidation ability, the synergistic effect on the oil of critic acid was better than that of isoascorbic acid. When 0.02% TBHQ with 0.005% BHA and 0.01% critic acid or with 0.005%BHA and 0.01% isoascorbic acid was used in the oil, the shelf life of the oil could be prolonged from 4 months to 11~13 months at 20℃.

结果表明,温度、时间对汉麻籽油的自氧化过程有显著影响,且温度的影响更明显;叔丁基对苯二酚对汉麻籽油具有较好的抗氧化性能,柠檬酸和异Vc对TBHQ与丁基羟基茴香醚复配的复合抗氧化剂均表现出较强的协同抗氧化性能,且柠檬酸的协同抗氧化性优于异Vc;添加0.02%TBHQ+0.005%BHA+0.01%柠檬酸或0.02%TBHQ+0.005%BHA+0.01%异Vc复合抗氧化剂,可使汉麻籽油在20℃下的预贮藏时间从4个月延长至11~13个月。

Based on the structure, those human diseases related mutation sites are mapped onto the structure accurately. As a result, it is found that these sites are all around the electron transfer prosthetic groups or ubiquinone. And these mutations will alter prosthetic groups' binding or ubiquinone's binding, causing electron leakage from the prosthetic groups in the electron transfer chain, producing reactive oxygen species and leading to tumor formation.

利用线粒体复合物 II 的结构对已知与人类疾病相关的该复合物突变位点进行了精确定位,发现这些突变位点均位于电子传递体或辅酶 Q 结合位点的周围,它们的突变或影响了电子传递体的结合,或影响了辅酶 Q 的结合,从而导致电子传递的中断,电子逃逸到线粒体基质中或线粒体内膜中,经过一系列下游途径导致疾病的产生。

An apparatus made by our lab is used for direct measurement of torque of yarns. The torque-twist relation curves, torque-relaxation behavior of several kinds of yarns at room temperature and heat-setting temperature are given. After analyzing the difference of different yarn torque it can be found that the special structures of composite yarns made of polyester filament and wool staple fibers mainly cause the high residual torque.

应用自行研制的直接测量纱线扭矩装置,这里测试数种纱线的扭矩-捻度关系曲线及其在常温和热状态下的扭应力松弛关系曲线,分析相同捻度下各种纱线扭矩差异的根源,发现涤长丝与毛短纤复合纱线的特殊结构是造成复合纱线残余扭矩较大的主要原因。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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