基因的

After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene. Moreover, the whole sequences of the two Wx genes of nonglutinous rice reported by others were also aligned, and another two pairs of dominant STS molecular markers were designed to discriminate Wxa from Wxb based on the special site between the two genotypes. Then, corresponding rice genotypes were identified by the established new markers.

通过对前人公布的糯性水稻蜡质基因全序列测定结果的分析，根据糯性水稻与非糯性水稻蜡质基因序列位点的差异，设计了两个基于PCR扩增反应的可特异识别糯性水稻的显性和共显性STS分子标记；同时在前人公布的非糯性水稻蜡质基因的不同类型的全序列比对分析的基础上，选取了两种基因型特定的差异位点，设计了两个基于PCR扩增反应用于特异识别这两种Wx基因的显性STS分子标记，并用新建立的标记对相应的水稻基因型进行了检测。

The interactions between the alleles controlling the crossability of wheat with rye may exist.

小麦可杂交性基因间存在互作，表现为纯合隐性等位基因对其它显性等位基因的抑制作用，而纯合隐性等位基因间的效应具有可加性。

The expression level of tim gene in 25LL is markedless higher than 15DD and 20LD in the early incubation stage(7 days before), but it is obviously higher than 20LD and 15DD conditions in the late.To further invesgate the per and tim expression in different moment every day of the sensitive period(7d,8d,9d) during the process of embryogeny,the expression of tim gene was very low and the per and tim expression did not have obvious rhythmicity under 15DD condition.

进一步调查家蚕胚胎对环境敏感时期(7d-9d)per和tim基因的昼夜表达节律，在15DD条件下，tim基因表达量一直很低，tim基因和per基因的表达量都没有明显的节律性变化；而25LL和20LD条件下，两基因在一日中不同时刻的表达具有明显的节律性，表达量也较高。

The distribution of hybrid chlorosis Ch1 gene and the T-type cytoplasm fertility restoring genes in Chinese endemic wheats were studied using the testors QA1104 (T-type cytoplasm sterile line, with hybrid chlorosis Ch2 gene) and Khapli emmer (with hybrid chlorosis Ch1 gene).

本研究以T型不育系QA1104（具有杂种黄化基因Ch2）和Khapli（具有杂种黄化基因Ch1）为测验种，对中国特有小麦等六倍体小麦类型和一些四倍体小麦类型中的T型胞质育性恢复基因和杂种黄化Ch1基因的分布进行了研究。

RESULTS We successfully obtained human caspase-3 gene, the genes and expression vectors of reversed caspase-3 and its fusion protein with part of the translocation domain of Pseudomonas exotoxin A. Compared with the control group, the expression of both genes caused in a similar degree the decrease of living cell numbers and massive occurrence of apoptosis.

结果成功获得了caspase-3基因，构建了重构型caspase-3基因和重构型caspase-3与绿脓杆菌外毒素转膜结构域部分肽段的融合蛋白基因及其表达载体，与对照组相比，在转染后1～4 d内，两种基因的表达均导致HeLa细胞存活数减少，大量细胞发生凋亡，且二者活性相当。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

By retrovirus vector the foreign mdr1 gene could be efficiently transferred into bone marrow mononuclear cells of mouse in vitro, and it was confirmed that mdr1 gene was expressing stably and effectively in cells; in short terms there is no significant influence on the bone marrow MNC by transfection of mdr1 gene,but in the long run(in our study we had surveyed 2 months),there is a decline in the expression of apoptosis factor Bax in the MNC which transfected by mdr1 gene,in favour of the recovery of the receptor mouse;on the other hand it also poses a blance problem between multiplication and apoptosis in the process of receptor hematopoiesis after transplanting HSC which was transfected with mdr1 gene.

通过逆转录病毒载体可在体外将外源性mdr1基因转入小鼠骨髓单个核细胞中，转染的mdr1基因能整合到骨髓单个核细胞基因组中并有功能性表达；mdr1基因转入小鼠骨髓单个核细胞在移植后短期内对受体造血细胞没有显著影响，但从长期看来(本研究观察2个月)转染了mdr1基因的造血干细胞的凋亡因子Bax表达降低，更有利于受体鼠造血的恢复；另一个方面它也同时提出一个问题，即mdr1基因转染造血干细胞在回输受体后造血过程的增殖凋亡平衡。

The structural, functional and informational concept of gene is not completely unified at all levels, the overlapping gene in the structural concept of gene which will be likely to definite a gene in the functional and informational concept of gene.

基因的结构概念、功能概念、信息概念并非在各个层面上都是完全统一的，基因的结构概念中提到的重叠基因，在基因的功能和信息概念中有可能就将其定义为一个基因。

To assess the effect of antisense Cyclin D1 on tumorigenicity,the tansfected cells were injected subcutaneously in ...

反义基因治疗是一种能有效调节肿瘤细胞中基因表达的手段，通过在转录或翻译水平阻断靶基因的表达，达到基因

It has been predicted by molecular genetics that the tumorigenesis due to one single gene is almost impossible, instead, it is always be a result of inactivation of several gene suppressors or activation of oncogenes.

近年来的分子遗传学研究从癌基因和肿瘤抑制基因的角度，提出单个基因的改变不能造成细胞的完全恶性转化，而是需要多基因的改变，包括几个癌基因的激活和两个或更多肿瘤抑制基因的丧失。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。