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A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein, Cp)序列设计引物,通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段,将目的片断克隆到原核表达载体pEGX-6p-1中,构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp,重组载体化大肠杆菌BL21,经IPITG诱导后,融合蛋白GST-cp得到了特异表达。

Thus Beauchamp was several days in getting two of his battalions and his headquarters and regimental troops moved.

因此,波查普在几天里一直忙于挪动他的两个营、团部和团直属队。

Chesty Puller and his regimental headquarters had joined Sutter at Koto-ri and in the next several days more Marine and Army units jammed their way into the protective envelope of the perimeter.

得意普勒和他的团部已经在古土里汇合,在随后的几天里有更多的陆战队和陆军部队进入了古土里防线的范围。

First I was transporting wounded soldiers on hey in the truck, and after that the regiment's liaison officer.

一开始我负责把运送伤兵,之后就开始负责接送团部的联络军官。

Now, for the first time, he gained radio contact with regiment.

在此时,他第一次和团部取得了无线电联系。

Actually, our company was under direct control of the regimental HQ.

事实上我们连现在直接受到团部的指挥。

I was a motorcycle driver for some time, a messenger with the regimental HQ.

一段时间我是一名摩托车手,一名团部通信员。

One was to advance along the MSR, to be followed by the regimental train and the reserve battalion.

一个营沿着主要补给线前进,后面跟着团部的车队和陆军的暂编营。

This is Doctor Kent, the regimental surgeon.

这位是肯特医官,团部的外科大夫

Litzenberg reinforced Harris with a composite unit, called "Jig Company," made up of about 100 cannoneers, headquarters troops, and other individuals.

里特森伯格派出了一支混编而成的部队对其进行增援,这支部队被称为&J连&,由100名炮兵、团部官兵以及其它人员组成。

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