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囊胚腔

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The strain of this virus was a strain which could not grow in chicken embryo and could not agglutinate chicken RBC.

此病毒为一种在鸡胚尿囊腔不生长,不凝集鸡红细胞的"O"相甲型流感病毒。

The results showed:(1) the cellule morphous and the disposition in chick embryo of PGCs were coincident no matter stained by PAS or HE staining, and HE staining could disclose the morphologic characteristics more clearly, exactly and completely;(2) genesis of blood island could be observed at the boundary of light and dark region of the extraembryonic blastoderm at about 26 hours;(3) both the blood vessel endothelium cells and free cells of the blood island were differentiated from PGCs. The generating of genuine yolk sac was at about 44-48 hours.

结果表明:①用经典的识别原生殖细胞的PAS染色方法与HE染色所观察到的PGC在鸡胚的分布及细胞形态基本一致,而HE染色较PAS法能更有助于清晰、完整、准确地识别PGC的细胞内部结构特征;②在孵化26小时的鸡胚胚外胚盘的明暗交界处可见血岛的发生,血岛发生的时间比真正的卵黄囊产生时间(大约44-48小时)要早很多;③血岛周围的血管内皮细胞与管腔中游离细胞都来自PGC。

Cleavage produces a blastula a sheet of cells rounded into a sphere that in most species surrounds a cavity.

卵裂产生一个囊胚,形成的细胞壁球形排列形成一个空腔。

The embryonic vessels failed to invade into the labyrinthine layer of placenta, which impaired the embryonic-maternal vascular connection. These defects could not be rescued by wild-type tetraploid blastocysts, excluding the possibility that they were due to the extraembryonic tissues.

组织免疫组化分析结果显示:毛细血管腔异常扩张,排列紊乱,不能形成大血管及细致有序的血管网络;胚外中胚层来源的卵黄囊脏层扁平上皮细胞形态变圆,与内皮细胞连接受损;胚胎血管不能侵入胎盘迷路层,胚体与母体血管连接受损。

In order to discuss the optimal condition in which GPMV proliferates in chicken embryo and its pathogenicity, GPMV of different titer was inoculated into 911-day chicken embryo and the allantoic fluid was harvested after 36, 48, 60, 72 and 84 h, the HA titer was measured as well.

为了探讨鹅副粘病毒在鸡胚中增殖的最佳条件及其致病性,将不同血凝滴度的鹅副粘病毒分别接种于9~11日龄鸡胚尿囊腔,收获孵化36、48、60、72和84 h的鸡胚尿囊液,测定血凝滴度。

Virus was also cultured in allantoic cavity of chicken embryo with statistically significant HA titer at 1:6.8 as compared with virus control group (P.01) incubated 24 hours after injected with MEK first then injected with IBV.

在鸡胚尿囊腔中,先注入MEK孵育24 h后,再注入B型流感病毒的鸡胚也培养出病毒,HA滴度为1:6.8,与病毒对照组比较P.01,有统计学意义。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示:未妊娠时,泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达,波形蛋白在子宫内膜基质细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,牦牛子宫任何部位均不表达角蛋白7;妊娠30天左右时,泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达,波形蛋白在子宫内膜基质细胞和内胚层细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,角蛋白7在尿囊细胞内表达,偶尔在腔上皮细胞的细胞核边缘表达;消化法进行原代培养时,组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞;分离得到的子宫内膜基质细胞活率达90%以上,并可在体外传代7次以上;分离得到的子宫内膜腺上皮细胞活率可达85%以上,并可在体外传代5次以上;RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长,维持子宫内膜基质细胞正常生长的FBS添加量为20%,维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Results There were 8 angiomyolipomas, 2 leiomyomas, 2 multilocular cystic nephromas, 1 hemangioma and 1 mesoblastic nephroma. Only multilocular cystic nephroma and leiomyoma have special characteristics that can be determined from RCC.

典型的平滑肌瘤和多囊性肾瘤在CT上有可与肾癌鉴别的特征性表现:平滑肌瘤为规则圆形,边界清楚,密度均匀,CT值略高于肾实质,无钙化,弱强化;多囊性肾瘤为边界清楚的包块,可见边缘锐利含多个分隔的肿块,分隔之间为囊腔,囊内容物的CT值与水相似或稍高,而错构瘤、海绵状血管瘤、慢性肾脓肿、中胚层肾瘤在CT上无法与肾癌鉴别。

These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位,同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值,本研究将pEGFP-N1基因导入小鼠ES-D3细胞系,得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP,通过对昆明小鼠的囊胚腔注射,获得了4只表达绿色荧光蛋白的嵌合体小鼠。

These orient themselves so that a fluid-filled cavity, the blastocoel, forms inside the mass of cells

这些细胞进行自我调整,以致在细胞群内部形成一个充满液体的腔,即囊胚腔

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