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The serum cretonne, serum BUN and uric acid in the patients were measured during 8 weeks. The model of CRF was induced by 5/6 nephrectomy in SD rats. Rats were divided into three groups: Sham group, 5/6 nephrectomized group, 5/6 nephrectomized and calcium dobesilate therapy group.

动物实验部分:建立5/6肾切除大鼠慢性肾衰模型,设立假手术组、5/6肾切除组以及羟苯磺酸钙组,治疗12周后观察大鼠肾功能以及残肾组织病理改变。

Contrast-enhanced Ultrasound(1) Renal occupied lesions: the contrast-enhanced imaging feature of renal cell carcinomas are multiformity. It was different in time of start-enhancing and start- expurgation and intensio.3 cases revealed wash-in contemporarily and 6 cases revealed wash- in quickly in time of start-enh ancing.In time of start-expurgating, 3 cases showed wash-out quickly, 3cases revealed wash-out contemporarily,3 cases showed wash-out slowly,6 cases revealed a higher contrast enhancement and 3 cases revealed a even contrast enhancement in enhancing intension; 4 cases with renal cyst s,outline had more clear and inside had no contrast media washing in and enhancing;3cases with renal angiomyolipomas washed in slowly,washed out slowly and equal or low enhanced ; 2cases with renal columnar hypertrophy were no outline appearance and the same enhancement and ech o as the nomal rena tissue.

超声造影诊断结果:(1)肾占位性病变:9例肾透明细胞癌,其超声造影表现多种多样,造影后肾脏恶性病灶造影开始增强的时间、开始廓清的时间及增强强度不等,在开始增强的时间方面,与正常肾皮质同时灌注增强的有3例,表现为快进的有6例,在开始廓清的时间方面,快退3例,同退3例,慢退3例,在增强的强度方面,6例病灶表现为高增强,3例为等增强表现;4例肾囊性病灶,超声造影轮廓较二维超声清晰,但内部始终未见增强;3例肾血管平滑肌脂肪瘤表现为慢进慢退,回声呈等、低增强表现;2例肾柱肥大超声造影无明显包块轮廓出现,造影增强的动态变化时间、强度与正常肾组织一致。

After being treated with Nephritis NO.1 Prescription, NO and the ET-1 contents in Model group recovered(P〈0.05)2、Nephridial tissue examination indicated that there were lessened microscopic structural pathological changes ,cellular proliferation and widening mesangial region in mesangial cells through treatment compared to Model Control , Conclusions :1 For Mesangial Proliferative Nephritis , Manshen NO.1Prescription has satisfactory therapeutic effects, It can improve symptoms, restrain accrementition in intercapillary cells and mesangial region, thus prevent nephric tubule's indurascent and prevent renal failure.2、Manshen NO.1 Prescription can increase NO contents; reduce ET-1 contents in Model control.

结果:1、与正常对照组相比,模型组血清NO含量减低(P〈0.05),而ET-1的含量升高P〈0.05〉,给予慢肾1号方治疗后,可明显提高系膜增生性肾炎模型组血清NO(P〈0.05〉,减低血清ET-1的含量P〈0.05〉,2、肾组织学检查显示:经慢肾1号方治疗后,与模型组相比,肾小球系膜细胞增生及系膜区增宽等超微结构病变均有所减轻。

Methods: Between November 2003 and December 2005, 67 patients with renal cell carcinoma underwent retroperitoneal laparoscopic radical nephrectomy (34 patients, group A) and open radical nephrectomy (33 patients, group B). Operating time, blond loss, amount of postoperative drainage, time to ambulation, recovery of intestinal function after operation, the postoperative hospital stay, use of antalgesic and transfusion blood and plasma were compared between group A and group B, retrospectively. Results: In group A, the operations of 31 patients were successful and 3 cases failed, then conversed to open surgery. Firstly, one case were not decented the prerenal fascia and the other two cases occurred complications, one case was due to injurying genital gland vein, with blood loss of 500m1, another fatty renal capsula was too thick to explose the renal pedicle. The operations of group B were all successful, only one case was died of intracerebral hemorrhage after two monthes.

回顾性分析和比较后腹腔镜肾癌根治性切除术(Retroperitoneal laparoscopic radical nephrectomy, RLRN)34例与开放性肾癌根治性切除术(Open radical nephrectomy, ORN)33例,比较两种方法的手术时间、术中出血、术后引流、术后最早下床活动时间、肠功能恢复情况、术后住院时间、术后镇痛剂使用量、术中术后输血、血浆量、术后随访时间及手术效果结果:A组中1例因最初对解剖结构难于辨认,无法分离肾前筋膜至肾门而改行开放手术,1例因损伤精索静脉出血难以控制而改行开放手术,1例因肾周脂肪过多,无法将肾脏掀起显露肾蒂而行开放手术,31例手术成功。B组手术均获成功,1例术后2个月因大面积脑出血死亡,余均无瘤生存。

Take the blood specimen to measure the hepatic and renal function and the tissue biopsy after the active and motive abnormities called toxical manifestation such as posture changes, abnormal sound, uneasiness, quiet, thrill, ataxia, convulsion and so on with toxic dose. Measure the tissue biopsy after death with lethiferous dose. If no toxicity or death occur after the experiment of 12 weeks, measure the hepatic and renal function and the tissue biopsy. There was no rat which had the toxical manifestation such as active or motive abnormities and 3 rats died because of environment or others after the experiment of 12 weeks.

实验中分别测定治疗剂量组小鼠在用药4周,8周,12周后的肝肾功能,并进行组织病理检查;中毒剂量组小鼠在出现行为、动作异常,如改变姿势、叫声异常、不安、安静、震颤、运动失调、惊厥等中毒表现时,取血液标本测定肝肾功能,并进行组织病理检查;致死剂量组小鼠发生死亡后进行组织病理检查;若12周实验结束时无小鼠出现中毒表现或死亡,则取血液标本测定肝肾功能,并进行组织病理检查。12周实验结束时,无小鼠出现动作、行为异常等中毒表现,有3只小鼠因为环境或其它原因发生死亡,病理检查结果正常。

Methods: 37 patients with complex upper urinary lithangiuria with pyonephrosis were selected during November 2002 to July 2007. 8 cases were treated with transurethral ureteroscope lithotripsy followed by tube internal drainage, while 29 cases were treated with minimally invasive percutaneous nephrolithotomy. After 714 days, minimally invasive percutaneous nephrolithotomy was employed for the second therapy.

选择2002年11月~2007年7月复杂上尿路结石合并脓肾患者37例,采用经尿道输尿管镜取石后置管内引流8例,微创经皮肾造瘘外引流29例,术后7~14 d后,2期行微创经皮肾镜治疗复杂肾结石。

Result: Comparison between EG and CG shows a significant difference in level of blood kidney function EG' s nephridial tissue appears nomal in structure and shows a clear struchural core without change in its nature.

结果EG与CG相比,血中肾功能水平均有显著性差异;EG肾组织结构正常,结构核清晰、无变性;CG组大鼠运动力竭24h后则出现肾组织损害、肾组织结构紊乱、肾区小管有脱落管型生成等。

Methods A retrospective analysis was performed in 14 cases of urinary obstruction among 1090 cases of transplanted kidney in the year 2000-2006, including 9 cases of vesicoureteral anastomotic stricture, 6 of whom received a second vesicoureteral anastomosis, one had infection surrounding the renal graft and ureter end necrosis, two ureteral anastomosis with bladder muscle flap, and one stenostomia aerocyst distention under ureteroscope.

回顾总结2000-2006年我院1090例肾移植患者中的14例移植后上尿路梗阻患者,其中输尿管膀胱吻合口狭窄9例,6例行膀胱输尿管二次吻合手术,1例移植肾周感染输尿管末段坏死采用移植肾近端新鲜存活输尿管与自体输尿管吻合,1例采用膀胱肌瓣代移植输尿管,1例采用输尿管镜下气囊扩张后放置双J管。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后,血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少,Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少,血小板聚集程度及红细胞聚集指数显著上升,病理切片显示胸主动脉内皮细胞破坏明显,脑心肾组织内的血管进一步增厚甚至几乎阻塞,导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR,脑体重比显著升高;心肌内出现明显的缺血梗塞疤痕灶,心体重比显著升高;肾小球明显萎缩、玻璃样变性,肾小管也变性甚至坏死,使肾硬化程度显著增加呈颗粒性固缩肾,肾体重比也显著减少。

There was significant difference in signal intensity between the cell concentration group and unlabelled cell group during 2 weeks. And significant difference occurred in the 5×105 SPIO-labeled cells per milliliter medium group and unlabelled cell group during 3 weeks.3. In kidneys of ischemia-reperfusion injury,the labeled BMSCs were demonstrated as signal intensity loss in renal medulla on T2*WI sequence in days 1,3,and5.The signal intensity loss was visualized up to 8 days after transplantation,and the signal intensity in the third day was the lowest. Histological analyses showed that most Prussian blue staining-positive cells were well correlated with the area where a signal intensity loss was observed in MRI.

在此细胞浓度的基础上,标记后细胞信号在T2*WI序列的降低在2w后失去统计学意义;而在细胞浓度为5×105/ml时,标记3w后信号在T2*WI序列的降低才失去统计学意义。3缺血再灌注肾损伤家兔细胞移植后第1、3、5天肾脏髓质区信号强度明显下降,至移植后第8天信号改变不明显,其中第3天信号改变最为明显;组织学分析见绝大多数普鲁士蓝染阳性标记细胞分布于肾小管周围,与MRI信号强度下降区域基本一致。

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