后柱

This article presents the corresponding measures on the adjustment of samplc pressure,temperature of thermotank ,flowrate of carrier gas,length of measuring cycles and determination of keeping time of sample compositions;the influence of sampling,columns interchange,backflush and time change of compensation valvc to the measurement and analysis and the solutions to bo adopted.

介绍 PGC3 0 2在线气相色相谱仪因原进口色谱柱劣化，换用国产色谱柱后，在调试过程中，样品压力、恒温箱温度、载气流量、测量周期长短、样品各组分保留时间的确定，采样、柱切、反吹、平衡阀门开关时间的改变对测量分析的影响及解决办法。

After the biomechanical finite element analysis for the three-dimensional reconstruction model, we find the structure of the deformed nose showes the following characteristics: 1, Nasal septum, nasal columella, lateral feet of nasal alar, lateral nasal dorsum are stress concentrated regions, that is the key mechanical positions of deformed nose; 2, Under the static state, nasal deformation has a very small value of stress distribution, under displacement load status, the deformed nasal has a large value of the stress distribution; 3, Ipsilateral nasal columella are the the key mechanical parts of deformed nasal rectify. And lateral nasal alar is also the most stress concentrated areas.

对于重建后的单侧唇裂鼻畸形鼻部模型进行有限元生物力学研究，发现畸形鼻的力学结构呈现以下特点：1、鼻中隔、鼻小柱、鼻翼外侧脚、鼻背外侧是单侧唇裂鼻畸形应力分布的集中部位，也就是畸形鼻部的力学关键部位；2、在静态状态下，畸形鼻部拥有的应力分布值很小，在位移载荷状态下，形变后的畸形鼻部拥有的较大的应力分布值；3、单侧唇裂鼻畸形整复中，鼻小柱患侧部是畸形整复的力学关键部位，其次就是患侧鼻翼部。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

After the structure was optimized, the intensity utilization coefficient of horizontally side-post, arch steel tube and mid-post increased 18%, 3%and 25%respectively, the vertically side-post which was utilized insecurely and insatiably utilized securely, stably, and reasonably, and steel of main body was saved about 3.7%.

优化设计后的连栋塑料温室，其横边柱、拱架和中柱的强度利用率分别提高了18%、3%和 25%，纵边柱由非安全失稳利用转向稳定安全性合理利用，主体结构节省钢材3.7%。

In the test research of the most radical plane column-beam joint specimens are done, so the feasibility and the problem for this construction method could be discussed.

基于此，本文有针对性的对轴压比、剪压比及梁柱混凝土强度级差进行控制，设计并完成了7个按梁混凝土强度浇筑的钢筋混凝土平面框架节点的低周反复加载试验，通过量测各试件节点区水平箍筋以及贯穿节点的梁、柱纵筋在不同受力阶段的应变等，综合分析了各个试件的延性、耗能性及梁柱纵筋的粘结退化状况，考察了节点核心区采用梁混凝土浇筑后对试件综合抗震性能的影响。

Through ethanol deposition, organic solvent extraction, then active carbon, 732 positive ion-exchange resin and silica gel columniation chromatography, cream nematocide substance have been obtained. Inspection the substance by TLC, UV-Visible Spectrophotometer have been deduced singleness component, and it's pure 97.38% by HPLC. The substance could use for structure analysis and character identify.

活性物通过无水乙醇沉淀，有机溶剂萃取，活性炭柱、732型阳离子交换树脂柱及硅胶柱层析后，经硅胶薄层层析、紫外－可见光检测和高效液相色谱检测，其纯度达97.38%，可用于物质进一步结构分析及性质测定。

Based on the above experiments, the author studied the mixed metallic pillared and modified montmorillonites. The experimental result indicated that the microporous structure of Fe-Al-PLMs was affected by the changing of interlayer Fe-Al polymer with different Fe/Al ratio. But the presence of Al iron was helpful to incorporate Fe iron into interlayer of montmorillonites.. Introducing Zr helped Al-PLMs getting better larger spacing, thermal stability, surface area and pore volumes. Its successful to apply Zr-Al-PLMs to treat wastewater containing Cr iron. The experiment of modifying Al-PLMs by PVA showed that the order of adding starting materials (Al-pillaring solution, PVA, montmorillonite solution) was related to the property of Al-PLMs. The PVA as precursor was beneficial for Al to go into interlayer of the clay and to form larger basal spacing porous clay. The surface area and pore volumes of Al-PLMs increased mainly because of increasing the external surface area and mesoporous volumes after acid-activating. This conclusion confirmed that the microporous structure of pillaring clay depends upon the interlayer pillar.

实验结果表明：在Al-PLMs中引入Fe离子，不同Fe／Al比制得的Fe-Al-PLMs因层间的Fe-Al聚合离子结构不同而影响其微孔结构，但羟基Al离子的存在有利于Fe离子进入蒙脱石层间；在Al-PLMs中引入Zr离子，有助于其层间距、比表面积、孔体积增大，所得Zr-Al-PLMs应用于含Cr废水处理效果显著；PVA改性后的Al-PLMs其物化性质变化与铝柱化剂、PVA溶液、蒙脱石溶液三者不同添加顺序有关，说明PVA的前撑有利于Al进入蒙脱石层间得到较大层间距的Al-PLMs；经酸化处理得Al-acid-PLMs，其比表面积增大以外表面积为主，孔体积增大以中孔为主，这也证实了柱撑蒙脱石的微孔结构取决于其层间的柱化物。

Methods: The sample that pass through handled to be abstracted twice by acetonitrile, evaporate the organic phase to dryness following defecated through the Na2SO4, cleaned-up by norm alhexalle, then analysed by HPLC (C18 column) with a gradient system of methanol-water as a mobile phase.

经处理过的样品用乙腈提取两次，将乙腈过无水硫酸钠层后挥千乙腈，正己烷洗涤净化，经过净化后进行高效液相色谱仪测定（C18柱），本方法以甲醇、水为流动相梯度洗脱，柱温35℃，在波长275 nm处测定。

The main works are summarized as follows:(1) A new hybrid heating method, namely heating using an electric furnace and blowing liquefied petroleum gas fire into the the furnace simultaneously, was used to heat the CFST columns and to simulate the real fire attack.

本论文对受火灾全过程作用后的钢管混凝土柱滞回力学性能进行了试验研究，以期为火灾后钢管混凝土柱的损伤评估和修复加固提供参考。

Its include: barrel, set up in the barrel components of the screw, fuel tanks, motor oil, the radio front plate, Radio Taiwan after the board and guide column, referred to the radio through the bottom of the front plate connected to the guide block guide Column, referred to Taiwan after the radio connected to the rod on the board.

其包括：机筒，设置在机筒元件中的螺杆，油缸、油马达、射台前板、射台后板、引导柱，所述的射台前板通过下端的引导块连接在引导柱上，所述的射台后板连接在活塞杆上。

The big eared hedgehog, the little owl, the sand gecko, the three-toed jerboa, and every show needs a villain - saw-scale - the viper!

大耳刺猬，小猫头鹰，沙壁虎，三趾跳鼠，每场戏都需要反派角色：蝰蛇。

Plasmodiophora brassicae ; resting spores ; density gradient centrifugation of sucrose ; ultrasonic disruption

根肿；休眠孢子；蔗糖密度梯度离心；超声波破碎