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The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

METHODS 80 eyes of 47 patients with glaucoma (31 eyes of 18 patients with POAG , 27 eyes of 14 patients with NTG and 22 eyes of 15 patients with CACG ) and 44 normal eyes of 39 control subjects were tested by simultaneous ICGA and fluorescein angiography with Heidelberg retina angiograph. The occurrence and extent of peripapillary atrophy and indocyanine green angiographic anormalies of optic disk were compared between glaucoma and control group, and among the three types of glaucoma as well.

应用Heidelberg共焦激光扫描眼底血管造影仪(Heidelberg retina angiograph，HRA)，对青光眼患者47例80只眼(POAG 18例31只眼；NTG 14例27只眼；CACG 15例22只眼)及正常对照组44例44只眼行吲哚青绿眼底血管造影(indocyanine green angiography，ICGA)和荧光素眼底血管造影(fundus fluorescein angiography，FFA)同步检查，比较青光眼与对照组及青光眼各组间视盘旁萎缩(peripapillary atrophy，PPA)以及视盘ICGA表现的差异。

Fundus photographs showed myopic chorioretinal atrophy in eight study eyes (47%), including diffuse atrophy in four eyes (24%) and patchy atrophy in four eyes (24%). The IS/OS defects on FD-OCT were accompanied by myopic chorioretinal atrophy in three (60%) of five eyes.

眼底成像显示8只眼（47%）有近视性脉络膜视网膜萎缩，其中包括4只眼（24%）为弥漫性萎缩，4只眼（24%）为斑块状萎缩。5只眼中有3只眼（60%）的傅立叶域光学相干成像显示为光感受器内外节缺损伴随近视性脉络膜视网膜萎缩。

Patients with ischemic CRVO have more severe situations and high risk factors of later neovascular complications occuring, the surgery of via a pars plana vitrctomy combined chorioretinal venous anastomosis can be the newest attempting treatment.

1对象；选择成年有色家兔19只38只眼，排除死亡2只4眼和术后影响观察的浙江大学博士学位论文并发症2眼，共计入选17只兔子32只眼球，被随机分成正常组2眼，对照组8眼和3个治疗组单纯穿刺吻合组(T a6眼：吻合联合不吸收材料植入组6眼：吻合联合可吸收材料植入组10眼。

IL 1β and IL 6 levels in supernatant of homogenates of cerebral cortices were measured by the Enzyme Linked Immunosorbent Assay.

28只C57BL/6J雄性小鼠接受MPTP 25 mg/kg ip注射连续5 d，对照组（10只）注射生理盐水，在最后一次注射的第1（10只），第3（8只）和第14日（10只）杀鼠，然后用酶联免疫吸附的方法检测大脑皮质匀浆液上清内细胞因子IL 1β和 IL 6的水平。

Secondary glaucoma developed in 6 of 9 eyes subsequent to iris atrophy (4 eyes) or a cyclitic membrane (2 eyes), and 3 eyes underwent trabeculectomy.

白内障囊外摘除术在角膜移植术同时或之后予以实施。9只眼中有6只眼发生了继发性青光眼，其中4只眼是由于虹膜萎缩，2只眼为睫状体炎性假膜。3只眼被予以小梁切除术。

Postoperative manifest refraction was also improved by surgery, showing minimal but significant regression after 1 year. Final BCVA loss was seen in 10 eyes of 7 patients; in 7 cases, there were obvious reasons such as the progression of cataracts in 3 eyes, epithelial disintegrity due to dry eye in 2 eyes, irregular astigmatism due to flap striae in 1 eye, and age-related macular dystrophy in 1 eye.

手术后的显然验光视力也有提高，在1年后表现为极微量却显著的回退。7例患者的10只眼发生了最佳矫正视力的下降，但均有明显原因，如3只眼发生了白内障，2只眼角膜上皮不完整，1只眼由于角膜瓣皱褶导致不规则散光，1只眼为年龄相关性黄斑萎缩。

BMPR-IB gene as a fecundity candidate gene was studied in Cele sheep(44 blood samples), Duolang sheep(49 blood samples), Hu sheep (32 blood samples),Chinese Merino monotocous line(40 blood samples) and Chinese Merino (47 blood samples) by PCR-RFLP.

本研究以绵羊BMPR-IB基因作为多胎候选基因，采用聚合酶链式反应—限制性片段长度多态性技术，对策勒羊(44只)、多浪羊(49只)、湖羊(32只)、中国美利奴羊多胎类型(40只)以及中国美利奴羊(47只)进行了分析研究。

Results The uplift of retinal neuroepithelium, separation or abnormal band of retinal pigment epithelial were found in all eyes.

神经上皮脱离腔内有细小尘状反光点36只眼，占39.14%。其中簇状分布17只眼（17.61%），柱状8只眼（8.69%），环形6只眼（6.52%），团块状5只眼（5.43%）。

Results The abnormal optic nerve was found in 44 eyes, in which, the increase in diameter of optic nerve was found in 38 eyes, in which, 22 eyes were accompanied with papilloedema, optic nerve hydrocele in 18 eyes, and decreased in diameter six eyes.

结果 B型超声检查发现视神经异常44只眼，其中视神经直径增大38只眼，伴视盘水肿22只眼，伴视神经鞘膜积液18只眼；视神经直径变小6只眼。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。