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It showed that the cumulus cells did play significant role in the stimulation of nuclear maturation of porcine oocytes and subsequent development.

表明卵丘细胞对猪卵母细胞的核成熟及卵母细胞体外受精后的卵裂和早期胚胎发育,都具有明显的促进作用。

Toreniafournieri. has a naked embryo sac that protrudes from the micropyle into the cavity between the funiculus and placenta. As embryo sac is mature, the egg cell, the two synergids and about half the central cell are located outside the micropyle.

蓝猪耳在其发育的四核期,胚囊从珠孔端伸出,当胚囊完全成熟时,助细胞、卵细胞和大部分中央细胞均暴露于胚珠组织之外,是研究被子植物受精和胚囊发育的模式植物。

In the late of April of the second year, male inflorescences unbound and pollinated. When female inflorescences accepted the pollen grains, ovary development was increased in early May. With the development of either of the two ovule, funiculus, integument and nucellus were differentiated. Embryo sac mother cell came into being and the meiosis begins. Then fuctional megaspore developed into matured embryo sac that contain eight nucleuses. In the end of May, double fertilization generated.

次年,4月下旬,雄花序解螺旋散粉,雌花序开花授粉;5月上旬,雌花序授粉后,迅速增长,子房膨大,两个胚珠分化出珠柄、珠被和珠心,珠心中产生孢原细胞;5月中下旬,大孢子母细胞形成并减数分裂,功能性大孢子形成成熟的八核胚囊;5月底,发生双受精。

It was a creative research method that by means of the culture in vitro of unfertilized ovary of strawberry to induce the gynogenesis and obtain the gynogenic plants with half number chromosomes.

首次对草莓未受精子房进行了离体培养,诱导了雌核发育,并获得了染色体数减半的胚囊植株。这是继草莓花粉植物后,开辟的获得草莓单倍体植株的又一条途径。

The main factors influencing the gynogenesis of the unfertilized ovary of strawberry were genotypes, kinds and concentrations of hormones, sucrose concentrations, the time of cold pretreatment, growth environment and development state and culture conditions.

基因型、激素的种类和浓度、蔗糖浓度、低温预处理时间、材料生长环境及发育状况、培养条件等是影响诱导草莓未受精子房雌核发育的主要因素。

At ld before anthesis, a biggest vacuole located at the micropyle end of the cell and its nucleus was pushed toward the chalazal end of the cell, which made an evident cellular polarity.

受精后合子中的钙颗粒又明显增多,在核质中分布一些较大的钙颗粒,在珠孔端大液泡中聚集了较多的絮状钙。

In the mature embryo sac, the synergid filiform apparatus, the polari-ties of the egg and zygote and the two polar nuclei are significant.

在授粉后18-24小时,卵细胞开始受精,在授粉后54-58小时,合子开始分裂,但初生胚乳核则在授粉后32小时已开始分裂。4。

Three kinds of embryos' chromosome, including goat fertilization embryo, goat-goat cloned embryo and goat-rabbit cloned embryo were analyzed, The results showed that all of them have 60 chromosomes with same character, all were telocentric chromosome, besides the X-chromosome is the second larger telocentric chromosome.

经对超排获得的山羊受精胚、山羊同种克隆胚和羊-兔异种克隆胚的囊胚期胚胎染色体分析表明,三种来源的胚胎其染色体数目均为60条,且所有常染色体均为端部着丝点染色体,X染色体为第二大的端部着丝点染色体,说明山羊同种克隆胚和山羊-兔异种克隆胚均为山羊的核型,是供体细胞核在不同胞质中的重新编程的结果。4。

Could be observed on 10th day after pollination, archesporial cell on 20thday, megasporocyte on 24th day, the functional megaspore on 30th day; and 8-nucleate embryo sac on 41th day. And then double fertilization finish and early globular embryo emerg.

授粉10 d出现指状突起,20 d形成胚珠原基,24 d大孢子母细胞发生,30 d功能大孢子形成,41 d发育为成熟的八核胚囊,而后完成双受精发育成早期球形胚。

Morphology of sexual reproduction abortion in'High Noon', a distant hybridization cultivar of tree peonies(P.delavayi var. lutea×P. suffruticasa), was systematically investigated with light microscopy and cross. The results indicated that:(1)The stamens matured earlier than the gynoecia and pollens dispersed at the early stage of anthesis but the rate of normal 2-celled pollens were merely 2.41%.

采用光镜和杂交等方法对牡丹远缘杂交一代品种'正午'有性生殖败育进行系统形态学观察发现:(1)雄蕊较雌蕊先成熟,初花期即散粉,正常2-细胞花粉仅占2·41%,且花粉在多种培养液中、在自身和其它两种柱头上均不萌发;柱头在谢花期大量分泌粘液,进入最佳授粉状态;(2)同一胚珠中多个大孢子母细胞减数分裂高度不同步,胚囊发育与受精延迟,部分胚乳游离核在细胞化时期分裂异常;(3)自花及品种内异花授粉不结实,天然授粉结实率为0·053‰~0·130‰。

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