受精卵核

Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接，并接入表达载体pcDNA 3.1中，构成含hMCP-DAF的真核表达质粒(pcDNA-MD3)；以脂质体转染猪内皮细胞，人血清处理阳性克隆细胞，鉴定抑制人补体溶破的功能；应用显微注射将线性基因导入猪受精卵；PCR检测导入基因整合率。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱，而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

German embryologist Hans Spemann envisioned an experiment—removing the nucleus from an unfertilized egg and replacing it with one from a differentiated cell—in hopes of finding a solution to the long-debated issue "nuclear equivalency".

德国胚胎学家Hans Spemann设计出一个试验，即把未受精卵的细胞核取出，取而代之以分化过的细胞核，以期解决长期争论不休的"核等效"问题。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didn\'t changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didnt changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

Methods Regulation region of bovine β-lactoglobulin geneand full-length genomic DNA encoding hEPO were used to construct eukaryotic expression vector,and the transgenic goats were produced by pronuclear microinjection.

方法采用牛β-乳球蛋白基因调控元件和hEPO全长编码序列基因组DNA构建真核表达载体，应用受精卵原核注射的方法制备hEPO转基因山羊。

AIM: To establish a transgenic mouse model of Hi1tchirtson-Gilford progeria syndrome and observe the effect of mutant lamin A on the implantation and survival ability of injected mouse zygotes.

目的：制备Hutchinson-Gilford早老症转基因小鼠模型，观察突变的核纤层蛋白A对小鼠受精卵着床和成活情况的影响。

The head of the sperm cell and the nucleus of the egg cell then unite and form a new cell: the zygote.

然后，精子的头和卵子的核融合，形成一个新细胞：受精卵。

In order to establish mouse nuclei transfer technology, mouse zygote culture medium and activated conditions were optimized in vitro and enucleated method of MII oocyte were investigated in this study.

本研究通过对小鼠受精卵体外培养体系，MII期卵母细胞孤雌激活条件的筛选以及探讨MII期卵母细胞的去核方法为小鼠体细胞核移植的成功奠定基础。

On November 14th Nature, one of the world's leading scientific journals, published a paper about the creation of embryonic stem cells using a technique called somatic-cell nuclear transfer (basically, taking the nucleus from a body cell and putting it in an unfertilised egg).

世界前沿科学期刊《自然》于其11月14日的杂志上刊登了一篇关于如何通过体细胞核移植制造胚胎干细胞的论文（简单的说，体细胞核移植就是从体细胞提取细胞核后放入未受精卵中的过程）。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。