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Selected the best cultivate condition by cultivated on four different media under different conditions; Observated morphology perperties; Studied on biology perperties by germ tube testing, Carbohydrate fermentation testing, Tween testing and Esculin dissociation; lysis testing.

选择四种不同的培养基分别在不同温度条件下培养,选出最优培养条件;通过光学显微镜、电子显微镜观察菌落及菌体的形态学特点;通过芽管试验、碳水化合物发酵试验、碳水化合物同化试验、沙堡培养基生长试验、过氧化氢酶试验、七叶苷分解试验、吐温试验研究该菌的生理、生化学特征;应用PCR技术通过特异性的引物扩增得到菌属特异性的片段,最后测定扩增片段的核苷酸序列,从而进行菌种的分析、鉴定。

Methods: The time of primany fermentation of the negtive fermentation tube which is neither aicd nor gas was prolonged from 1824 h which. Is stipulated by nation criterion detection of coliform bacteria to 3648 h and 358 samples of 7 categories were investigated.

依据国标法检测大肠菌群数,将初发酵培养18~24 h未产酸产气的阴性管样品继续培养至36~48 h,对7类358份样品进行大肠菌群数的检测。

It was found that, there were more yeast biomass and more ethanol production in the presence of inositol and NaCl than without them.

经实验测定,肌醇和NaCl对嗜鞣管囊酵母产酒精能力及发酵的耐酒精能力均有显著的影响。

Objective To determine whether antibiotic resistance probability of Coliform bacteria in sanitary sewage is in existence. Methods Coliform bacteria has been separated from Sanitary sewage samples of Nanning Langdong sanitary sewage plant,and its antibiotic resistance has been detected to seven kinds of antibiotics,including Benzylpenicillin, rythromycin, Ampicillin, Gentamicin, Cefazolin, Amikacin, Cefotaxime.

目的 检测生活污水中肠菌群对当常用抗生素是否有耐药性方法采集南宁市琅东污水处理厂初沉池中的水样,用管发酵法分离其中大肠菌群,采用药敏试纸扩散法,检测了分离出来的大肠菌群对七常用的抗生素包括青霉素、红霉素、庆大霉素、阿米卡星、先锋霉素Ⅴ、先锋噻肟和氨苄西林的耐药性。

The contents of residual chlorine in the sewage samples were determined with iodometric titration method and the fecal coliform counts of the sewage were determined using multi-tubes fermentation method.

采集2003、2004、2005年和平区14家医院排放的污水,每季度监测1次,采用碘量法测定医院排放污水中余氯的含量,采用多管发酵法测定废水中的粪大肠菌群数。

We selected the 2nd and the 3rd generation yeast with powerful vitality from the yeast mud fermented in tanks in working field as the ideal species source separated from yeast monoplast in beer and the ways to obtain them were as follows: 24 hours after deoxidisation of diacetyl to threshold value and decrease of its temperature to 5℃ was the best time for selection and sample-extraction location was at the nozzle of pressure-discharge pipes.

摘 要:从生产现场大罐发酵的酵母泥中选取第2~3代生命力强的酵母作为啤酒酵母单细胞分离的理想种源,选取的最好时间为双乙酰还原至阈值后降温至5℃,并让其停留24h时取样位置为排压酵母的管口,最后将所得菌种进行初选、复选或模拟生产恒温培养鉴定,选出优良菌株。

Accordance with volume of biogas actually using requirement so that actualize supporting facilities, it can keep the pressure contant of biogas using for long time, the floating cover upside of body be pressurized, when the floating cover biogas digester fermenting to produce biogas will drive the soft PVC floating cover rising up with biogas storage, if user make use of biogas in case of biogas stove which the biogas pressure will be drop below in conjunction with floating cover will synchronous drop, when the floating cover has full of biogas which supernumerary of biogas automatic overflow to outside from bottom side of floating cover, it can not be appear with over high pressure thereby cause the tympanites of floating cove burst or biogas pipe burst and never has such phenomenon happen, the floating cove type biogas digester has reliable safe security.

根据沼气实际使用需求用量来做相应配置,可以保持长期在恒压状态沼气气压,浮罩顶部是密封的,当浮罩式沼气池发酵时所产生的沼气使浮罩上升并储存沼气,如果用户在用沼气炉灶用气时沼气压力将随之下降的同时浮罩也随着气压的下降而降低,当浮罩充满沼气时多余的沼气会自动从浮罩底部溢出,不会出现因气压过高造成鼓池、爆裂或爆管现象发生。浮罩式沼气池具有非常可靠的安全保障。

Cinnamomea raw material suspension and ethanol extract and hot water extract, respectively, the results showed a significant decrease of GOT, GPT and LDH levels in mice serum compare to the carbon tetrachloride group. The raw material group showed the significant inhibition of liver lipid peroxidation induced by the carbon trichloride radical.

在急性肝损伤实验方面:以胃管餵食樟芝固态发酵物原物料悬浮液及酒精和热水萃取物溶液后,由血液生化检查显示血清中GOT、GPT及LDH的浓度与四氯化碳组比较均有明显降低的情形;药物组在实验中显现了明显抑制因腹腔注射四氯化碳所产生的三氯化碳自由基 CCl3。

One L-glutamic acid high-producing strain TN6319 with genetic maker (FEA~r+KMA~r+SG~r ) was screened through shake tube primary screening ,shake flask second screening,genetic marker test and single clone isolation and continuous passage cultibation.this strain could produce L-glutamic 72.9g/L after fermentation 42h under the fermentation without being optimized.

经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代,最终筛选出一株L-谷氨酸高产菌TN6319(氟乙酸~r、酮基丙二酸~r、谷氨酰胺~r、磺胺胍~r),该菌株在未优化条件下发酵42h产L-谷氨酸79.2g/L。

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