发酵工艺

The results showed:(1) Through glycosylated orthogonal test, When the substrate concentration is 1:2, glycosylated temperature is 60℃, the enzyme dosage is 0.5%and glycosylated time is 6 hours, glycosylating was at best, and the sugar concentration was 33%(2) through fermentation orthogonal test, when adding the yeast is 2.0%, proportion of Rhodiola and glutinous rice is 1:1, the temperature is 28℃, and fermentation time is seven days, alcoholicity of the wine was the highest and sensory evaluation was the best..

本文立足加工高品质的红景天糯米保健酒，主要对糖化条件，发酵工艺参数的确定，后发酵温度的确定，稳定性试验，膜过滤试验条件，营养成分分析等方面进行系统的研究，结果表明：(1)通过糖化正交试验，在底物浓度1∶2、糖化温度60℃、酶用量0.5％、糖化6h时糖化效果最好，糖液浓度33％；(2)通过发酵正交试验，在酵母添加量2.0％、红景天糯米比1∶1、发酵温度28℃、发酵时间7天生成的酒度最高，感官评价最好；(3)通过后发酵试验，选择10～15℃温度下后发酵，酒体澄清，感官评价最好；(4)从对红景天糯米酒稳定性分析中得知：酒体在低于-6℃时略有絮状物出现，酒体混浊沉淀的主要原因可能是蛋白质。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

During the intermissive fed-batch process, the feeding medium was added 10 times separately to make the total amount of C and N sources equal to those added in the batch process, and the yield of maduramicin was improved to 9645 μgmL^(-1), which is 15.7% higher than that obtained during the batch process. In the continuous fed-batch process, the maduramicin yield reaches 10328 μgmL^(-1), which is 6.0% higher than that obtained in the intermissive fed-batch process, and is 29.4% higher than that obtained in the batch process.

在间歇补料分批发酵工艺中，分10次补加培养基，使碳、氮源的总浓度与分批发酵相同，马杜霉素发酵单位提高到9645μgmL^(-1)，比分批发酵提高了15.7%；在连续补料分批发酵工艺中，马杜霉素发酵单位可达10328μgmL^(-1)，比间歇补料分批发酵提高了6.0%，比分批发酵提高了29.4%。

The process and condition in the preparation of dextran through the fermentation of Leuconostoc mesenteriodes 0326 were discussed in the paper.The adhesive viscosity,pH value, the production of fructose and dextran,variation of molecular weight and the fermentation inducer were investigated,and the related curves of the dextran fermentation presented.

文章对由肠膜状明串珠菌L.M0326发酵生产右旋糖酐的工艺过程及条件进行了探讨，通过对其发酵过程中的粘度、pH值、果糖、右旋糖酐生成和分子量变化的研究及对发酵诱导物的考察，得到了右旋糖酐发酵工艺的相关工程曲线。

With different culture background, formed two different ferment technology,course mixed ferment and pure-blood ferment, mixed ferment was provided with the quality of multiple strain accretion, enzyme system mutual complementarity, simplified equipment, suited to produce composition complexity and exigent flavour traditional zymolytic food of our country,pure——blood ferment had the quality of strain singleness,rare pollution, suited to produce composition singleness,exigent puritg alcohol and monosodium glutamate.

中西方文化底蕴不同，形成混含发酵与纯种发酵两种不同发酵工艺路线的历程。混合发酵具有多菌共生，酶系互补，省工节能，简化工艺设备，克服中间生成物浓度过大的特点。宜于生产成分复杂，风味要求高的我国传统发酵食品，纯种发酵基质，菌株单一，污染少，宜于生产成分单一，纯度要求高的酒精，味精等产品。

Studied the effect of quality of the one step liquid to the second step fermentation rate and periods first time, confirmed the effect factor of the one step leavens process versus the second step.

首次研究了从一步发酵工艺的醪液质量对二步发酵的收率及周期影响，并确定了一步发酵工艺对二步发酵工艺影响因素。

And the optimum fermentation conditions were determined as follows: inoculation of 0.10 % YWBY in saccharifying mash, the ratio of material to water was 1∶1.0 and 9 d fermentation at 25 ℃ during chief fermentation stage, then 20 d fermentation at 22 ℃ in late fermentation stage.

确定草菇糯米甜酒的最佳发酵工艺条件：主发酵工艺条件为在糖化醪中接入0.10 %YWBY，料水比1∶0.8，25 ℃下发酵9 d；后发酵工艺条件为22 ℃下发酵18 d。

In order to ascertain Auto-circular fermentation technology of Mulberry vinegar and find out the vinegar 's ability to eliminate DPP H ., the liquid fermentation techniques of Mulberry vinegar, produced by using Auto-circular fermentor , have been analyzed and the micro-organism on the Mulberry fruit surface has been observed with microscope, the total flavones content as well as the free radical-DPPH eliminating ability in several kinds of vinegars have been tested.

为了确定桑椹醋自回淋发酵工艺，并了解其对 DPPH 自由基的清除能力，以秦巴山区桑椹为原料，采用液态自回流发酵法生产桑椹醋，从原料菌群、酒精发酵主产物和醋酸发酵主产物的动态变化趋势等方面对其工艺进行了分析，并采用比色法对桑椹醋总黄酮含量及其 DPPH 自由基的清除率进行了测定。

The present invention provides one simple and economic fermentation process for propylene glycol production.

其优点是发酵工艺简单经济，既简化了操作条件，降低了生产成本，又缩短了发酵时间，提高了生产效率，而且微氧条件下发酵液中1，3－丙二醇的浓度与厌氧发酵相当或略高一些，为微生物发酵法生产1，3－丙二醇的工业化提供了简单经济的发酵工艺。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。