发育完全的

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

It's the demanding director's work once more--he had ordered his actors to lose weight because "people in the 40s didn't look as full-figured as people of today."

这又是这位高要求导演的又一项"功课"—他曾对演员下了「要瘦」的要求，因为"40年代的人不会像现在的人那样发育得那么完全。"

The relative size of the hippocampal formation in relation with the total volume of the brain is often conserved in most of the mammalian species.

绝大多数的哺乳类动物海马的大小取决於脑容量的大小，但是鲸类这一部位的发育相对不完全。

The discovery of histopathology and ultramicroscopy was that cerebral cortex was agenetic, the structure of six layers was absent.

结果 1.NTDs胎儿的大脑皮质发育不完全，没有形成大脑皮质的6层结构，未见明显的神经细胞形成。

The results shows that the tapetal expanding abnormally occurs in dyadphase stage, microspore abortion occurs in the early of monokaryon stage, disaggregation in the end of monokaryon stage, only some spall of microspore is observed in monokaryon stage, the four cell walls also exist, the tapetal cells became completely vacuolated, almost filled the whole locule. vascular bundle of male sterile line anther vanish at mature pollen stage.

结果发现花药绒粘层在二分体时期发生异常，小孢子在单核前期开始败育，至单核后期完全解体，花药发育后期不育系药腔中只观察到少量的小孢子残体，花药壁的四层细胞依然存在，绒粘层细胞已经彻底液泡化，占据了药腔的整个空间，花粉成熟期不育系花药维管束消失。

It is detected by the Chinese Academy of Sciences Ocean: muscle contains more than 10 kinds of essential amino acids, fat containing 12.5%"DHA" and "EPA", to soften the heart and brain blood vessels, and promote brain development, improve the intelligence, prevention Alzheimer's disease has a good effect; cartilage and bone marrow (commonly known as "Long tendons") have anti-cancer factor, can be completely direct consumption, known as "Shark fin, bone sturgeon," said.

据中国科学院海洋所检测：肌肉含有十多种人体必需的氨基酸，脂肪含有12.5%的&DHA&和&EPA&，对软化心脑血管，促进大脑发育，提高智商，预防老年性痴呆具有良好的功效；软骨和骨髓有抗癌因子，可完全直接食用，素有&鲨鱼翅，鲟鱼骨&之说。

Despite its higher index of species diversity, the Reamuria soongorica community was not extremely stable because it had lost its ability of multiplying and resuming, it would be replaced by the community composted of herbages.

红砂群落草本层物种发育良好，导致多样性指数较高，但红砂种群是一个极不稳定的种群，已完全丧失了繁殖能力和恢复能力，取而代之的是以草本植物为主的群落。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。