双条件的

As early as Before Chin Dynasty, the Confucianists had developed the learning tradition that virtue and truth were emphasized equally. Their learning principle was self-cultivation in both benevolence and wisdom, i.e. moral inquiry.The learning tradition of ancient West mainly means the learning tradition created by Socrates and developed by Plato and Aristotle. It paid close attention to existence and the intrinsic consistence of knowledge and benevolence.The Enlightenment had dual influence on existentialism meaning of learning. For one thing, it created conditions for the liberation of human. The call for ration, freedom and democracy aroused inquiry spirit in education. For another,"subject-object" dualism looked oh scientific knowledge as rigid object truth, and human mind became "mirror" to reflect the objective reality. So education degenerated into knowledge indoctrinating and skills training. It depressed inquiry spirit and human creative personality.

先秦以来儒家的学习传统是强调真理与德性并重，儒家的学习原则是仁智双修，德性探究；古代西方的学习传统，主要是指由苏格拉底所开创，经柏拉图、亚里士多德继承和发展的关注人的生存、追求知识与&善&的内在一致性的学习传统；启蒙运动一方面为在教育中实现人的解放创造了条件，对理性、自由和民主的呼唤催生了教育中的探究精神，但另一方面，启蒙理性的&主-客体&二元论性格，使得科学知识变成僵化的客观真理，人的心灵变成反映客观实在的&镜子&，教育由此退变为知识灌输和技能训练的过程，压抑了探究精神和人的创造个性。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。