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The original method of acetic acid is influence of the color stability of the main factors.

原法乙酸是影响显色稳定性的主要因素。

Methods: The original method diacetyl monoxime solution concentration of 2 g/L to switch to 10 g/L solids volume direct add to antipyrine dissolved of solution;DMA plus 10 ml water 2 ml 1 h after boiling liquid cooling, Pure water volume to 25 ml determination.

将原法二乙酰一肟溶液浓度2 g/L改为10 g/L的固体量直接加到安替比林溶液中溶解;水样10 ml加DMA2 ml沸浴1 h后冷却,纯水定容至25 ml测定。

Thirdly, this dissertation presented a inverse analysis method for controlling the initial length of the cable.

本文提出控制索原长的反分析法。

The modified double weight function method is to introduce new weight functions doing subtraction between two negative exponential-type functions. For a Pearson type three probability distribution, the MDWF is able to make estimations of the Standard Deviation and Coefficient of Skewness C(subscript S from the second-order weighted central moments reduced to the first-order weighted central moment estimations.

修改的双权函数法是将两个负指数型函数之差所构成的函数类取作权函数,对于皮尔逊Ⅲ型分布,它能使σ和Cs的估计由原权函数法的二阶加权中心矩降低为一阶加权中心矩估计。

It was not cross- reaction with Entamoeba histolytica and Schistosoma japonica.It is a simply and better method to extract the DNA with lysine boiling.

结果 经扩增于500bp、670bp、1200bp出现特异性片段,与原选择的编码基因片段大小相同,此特异片段与溶组织内阿米巴、日本血吸虫DNA无交叉反应,不同的DNA提取法结果显示:用裂解煮沸法同样可从仅含1条旋毛虫幼虫的25μl正常小鼠血浆中扩增出特异性片段。

It is adopt a flexible and innovative pattern,exert the superiority of target language,interpret the idiomatic expression in accordance with target language on the basis of original brand meaning to left consumers a deep impression.

2意译法。意译法是商标翻译的另一种常见的方法,它是根据原商标的含义,采用灵活创新的方式,发挥目的语的优势,翻译符合目的语的表达习惯,给消费者留下深刻的印象。

As the objective function is not strictly concave in the path rate, the penalty function method is applied to transform the primal problem into a new formulation to obtain the optimal solution based on the subgradient method. And the multiPath congestion control algorithm is also proposed.

由于多径效用最大化问题中的目标函数对路径速率而言不是严格凹的,所以运用罚函数法将此最大化问题转化成新的等价形式,再运用次梯度法获得了原问题的最优解,由此提出了用于Ad hoc网络的多径路由优化拥塞控制算法MPCC。

The results show that homogeneous collagen dispersion can be gained by both methods. But only by the second method can we receive typical cross striation structure which was observed by EM. The solid content and viscosity of the collagen dispersion can be easily controlled.

结果表明:两种方法均能得到均匀的溶胀体,但用酸法提取的材料电镜观察为非横纹结构;而酶消化法提取的材料,电镜照片为典型的横纹结构,胶原溶液的固体含量、粘度等指标可控制。

Base on the initial study of the research project of"Study on treating hypertension of the aged with the treatment of tonifying the kidney qi", discuss the functional mechanism of the treatment of tonifying the kidney qi from 3 aspects, such as study on theory, documental commentation and experimentation.

在&补益肾气法治疗老年人高血压的研究&课题前期研究基础上,从理论研究、文献评述、实验研究(血压、主动脉胶原分布、含量的变化,NO、ET-1)三个方面探讨补益肾气法的作用机制。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法:1、培养原代的人RPE细胞,经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后,通过AO/PI染色技术确定培养细胞的存活率,描记其生长曲线,第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后,通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后,采用MTT法观察其对细胞的增殖力的作用;通过流式细胞仪观察其对细胞周期的影响;通过扫描电镜观察其对细胞形态的影响;通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用;采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率,评价其对细胞间通讯功能的影响;通过制作RPE细胞损伤模型,观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质,应用等电聚焦电泳和SDS-PAGE双向电泳技术,银染显示分离出的蛋白质斑点,经凝胶图像分析软件对两个样本进行胶图分析,寻找差异蛋白点。

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