原始生殖细胞

AIM:Studies on totipotential stem cells using primordial germ cells have the same application prospect as embryonic stem cells, especially for the animals difficult to isolate embryonic stem cells from blastula.

目的：应用原始生殖细胞进行全能干细胞的研究具有与胚胎干细胞同样广阔的前景，尤其适合于难以从囊胚内细胞团中分离培养胚胎干细胞的动物。

OBJECTIVED: To investigate in vitro and vivo biological characteristics of primary culture of human primordial germ cells on fibroblast feeder layer of human endometrium.

目的：观察接种于人子宫内膜成纤维细胞饲养层上原代培养的人原始生殖细胞在体内外的生物学特性。

Embryonic stem cell has the capability of immortalization and pleuripotent differentiation.

胚胎干细胞源于着床前的囊胚内细胞团或早期胎儿原始生殖细胞的一类未分化的多能性干细胞，具有无限增殖和多能分化的能力。

Objective To research on the proliferation and differentiation in vitro of primordial germ cells.

目的探讨小鼠原始生殖细胞的体外增殖与分化。

Isolation, culture and transgene of the chicken primordial germ cells will be processed in this research.

本研究将完成鸡原始生殖细胞的分离、培养及转基因的实验研究。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达，由原始生殖细胞产生卵原细胞，髓质退化成为卵巢腔，分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加，它们在分化结束后解体，分化前细胞内有较多的大颗粒，在分化中大颗粒减少，小颗粒增多，在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜，髓质有许多睾丸管构成，管腔内有散落排列的精原细胞。

Primordial germ cells were the progenitor cell of spermatozoon and ovum.

原始生殖细胞是精子和卵子的前体细胞。

In the paper, the PGCs' origin and migration of Varicorhinus macrolepis was studied, as well as the differentiation, development and maturation of gonad at microscopic and submicroscopic levels.

本课题对泰山螭霖鱼原始生殖细胞的发生、迁移及性腺的分化和发育规律，在显微和亚显微水平上进行了全面而系统的研究。

The result showed that mouse fetus liver cells condition medium,which can maintain survivance and proliferation of mouse EG cells,was better than the medium containing no cytokine,but worse than EG cell medium with 1 000 IU/mL of LIF.

同时，试验对比了从不同胎龄胎儿分离培养原始生殖细胞的情况，鉴定了EG细胞的生物学特性；EG细胞经体外培养，可以分化为神经样细胞、上皮样细胞和简单类胚体。

An endodermal sinus tumor of the testis is shown composed of primitive germ cells that form glomeruloid or embryonal-like structures.

睾丸的内胚层窦肿瘤由原始生殖细胞构成，这些细胞形成血管球或胚芽样结构。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。