卵球

We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略，通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定，以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定，对这种技术条件的优化、准确性和可靠性进行了研究。

The average amplification efficiency of single blastomere was 89.7％ and the average allele drop-out rate was 14.4％.

单个卵裂球平均扩增效率为89.7％，等位基因脱扣(allele drop-out， ADO)率为14.4％。

One embryonic cell can be separated into many blastomere cells. As be the nuclear donor of the embryonic cell nuclear transfer, it can breed the number of eminent species largely, increase the number of endangered animals, supply large number of supply same genotype animals, improve the efficiency of gene transfer and sex control and offer same genotype apparatus or organ for human transplant.

一个早期胚胎细胞可以分离多个卵裂球，以此作为核供体进行胚胎细胞核移植，可以大量扩繁优良品种的数量，增加濒危动物的数量，为科学研究提供大量遗传同质的动物，提高转基因和性别控制技术的效率，提供基因型相同的器官或组织供人类移植。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域，用限制酶 Bfm 消化 PCR产物，10 %聚丙烯酰胺凝胶电泳检测。

Use of a "cloning" procedure known as "artifical twinning" or "blastomere splitting" to create several genetically identical embryos from one, so long as the resulting embryos are experimented on and discarded instead of being transferred to the womb.

使用"克隆"程序称为"人工配对"或"卵裂球分裂"制造基因相同的胚胎数由一、只要是试行并导致胚胎被丢弃而不移送的子宫。

The blastula formation whose embryos were from different sources was compared and the blastomere number, quality classification and the relationship between the parity of the blastomere number and the blastula formation at d 3 were studied.

比较不同来源胚胎囊胚形成情况和d3胚胎的卵裂球数、质量分级等；并利用废弃胚胎囊胚形成情况对体外受精妊娠结局进行预测。

Methods Multiplex nested PCR was used to amplify the sex linked amelogenin and IL-3 genes from human single lymphocytes and single blastomere.

方法取成人外周血单个淋巴细胞和单卵裂球，采用多重巢式多聚合酶链技术同时扩增性别相关基因Amelogenin基因和IL-3基因。

Porcine was acted as the experimental animal. It was researched on problem of the origin of obtaining early embryonic cells, methods of separating blastomere cells, best parameter and order of ECNT embryo electric activation and system of ECNT embryo IVC, which was to find a scientific method of fitting ECNT and a procedure of fitting the lab producing embryos largely, optimize the procedure of ECNT and improve the ECNT embryo in vitro.

本实验以猪为实验动物，对猪胚胎细胞核移植供体的来源、卵裂球细胞分离方法、核移植重建胚的电激活最佳参数、核移植受体的激活时序以及核移植重建胚体外培养系统相关问题进行了研究，旨在探索一种适用于猪胚胎细胞核移植的科学方法，提高猪胚胎细胞核移植胚胎的体外发育率，优化胚胎细胞核移植程序。

Interestingly, a monopolar spindle was assembled in each blastomere in the second cell cycle, and disjunction of duplicated chromosomes and the cleaage was preented, leading to the chromosome set doubling.

有趣的是，在第二个细胞周期中，每一个分裂球中都组装了一个单极纺锤体，同源染色体分离和卵裂被阻止，导致染色体组加倍。

The cells of the blastula called blastomeres also each receive a full diploid set of chromosomes.

这些囊胚细胞，称卵裂球，每个细胞都有一套二倍体染色体组。

With the development of highway construction, the speed of cars has been gradually increased.

随着公路的发展，汽车的车速也在不断提高。

So we basically had to develop a bond with the animal in terms of socializing with it.

所以我们就主要想通过跟它沟通的方式来建立一种良好的关系。