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Genomic DNA of positive transformed plants; 6, 7: Genomic DNA of negative transformed plants; 9: genomic DNA of untransformed plants; 10: Positive control of 3G1MHpCAMBIA2301; 11: NTC; 12: DL 2000 marker.

2.2 转基因拟芥的筛选选择培育后获得转基因拟芥植株。从其叶片中提取微量DNA, PCR鉴定,可扩增出750 bp左右片段(图3),表明3G1MHpCAMBIA2301基因已整合到拟芥染色体中。

Some scholars believe that the Late Ming Dynasty carried out a double-recognition policy toward Annan but, through the analysis of the Late Ming Dynasty's diplomatic relationship with the Li and the Mo governments and its disposal of bonier disturbance, this thesis maintains that the Late Ming Dynasty did not break away from the One-Annan policy.

学界认为晚明这-政策是对安的&双重承认&,其实晚明对安的政策并没有脱离&一个安&的原则,并以此为基础处理对黎、莫关系以及边境骚动。

When the South hard, done everything to ensure that the occasion for their expiratory eyebrows; Yao south suddenly announced, Kens Fengdian has affected the image of television fame, and asked the South selling Ken, canceled his performances.

埋头苦干、倾尽全力,务求为自己吐气扬眉之际;瑶突然向宣布,Ken的疯颠形象已影响到电视台的名声,并要求出卖Ken,取消他的演出项目。

Recognising those whose extraordinary efforts have made the University a better place, reinvigorates the Nanyang spirit and the University's commitment to using its resources for the public good.

校友和各界人士的支持及义举,使大成为更理想的学习场所,表扬校友及外界同好为大额外付出的努力,将为大精神注入新活力,实现大学优化资源作育英才以造福社会的承诺。

By the analysis of the misty classification to the plant diversity of Nanyue nature reserve area and its 9 vicinities,The results show that:By their relationship of Nanyue nature reserve area and its 9 vicinities,the result is: Taiwan Area>Jinggang Mountain>Wuyishan Scenery>Daluo Mountain>Baiyun Mountain>BaiShan Mountain>BaiChuang Mountain>Huangshan Mountain>Xinnongjia.In order to do a beneficial tries and investigate a new plant classification method,and meanwhile the results is very meaningful to the management and reservation of Nanyue nature reserve area.

本文通过对岳自然保护区植物多样性与其相邻的其余的9个地区植物多样性进行模糊聚类分析,结果表明,按与其区系关系的密切程度的大小进行排列,与岳自然保护区的关系的密切从大至小的顺序为:台湾岛>井冈山>武夷山>大罗山>白云山>北山>北仑山>黄山>神农架,旨在对植物区系分类方法的研究作一个有益的尝试,探索一条植物区系分类的新方法,同时其结果对岳自然保护区的经营管理和保护具有一定的实践指导意义。

Within this segment the creeks and gullies that cross the fault were offset and the displacements range from several meters to about 5km.

野外调查表明,西藏东部的嘉黎断裂带从那曲到嘉黎由三段呈雁行排列,大致沿N60°W方向延伸,其东段在波密附近向转折,沿贡日嘎布曲向东过上察隅和下察隅后转为近北向延入缅甸境内与什阶断裂相连。

Cerevisiae have found that bZIP proteins play a role in yeast anti-oxidation. at3g51960, the transcription factor found in plants, is highly homologous to that of yeast. To examine the in vivo role of at3g51960, the overexpression and silencing vectors were constructed respectively. By using flower dip-dye, the binary expression vectors, pCAMBTA3301: at3g51960 and pCAMBTA3301: at3g51960Promoter, as well as vectors pCAMBTA3301: at3g51960-GFP and pCAMBTA3301: at3g51960cDNA were transformed to Arabidopsis by Tumefaciens. The transgenetic homozygous Arabidopsis strains have been derived after screening. Analysis on these strains reveals that strains overexpressing at3g51960 gene have higher level of stress resistance, implying a role of at3g51960 in plant stress regulation.

酵母中发现了bZIP家族的转录因子与酵母的抗氧化性相关,植物中的转录因子at3g51960与酵母中的这一转录因子在空间结构上有很高的同源性,为了验证它在体内的功能,本实验构建了过量表达载体和沉默载体,利用花浸染法把农杆菌携带的双元表达载体,pCAMBTA3301: at3g51960,pCAMBTA3301: at3g51960 Promoter和pCAMBTA3301: at3g51960-GFP及pCAMBTA3301: at3g51960 cDNA转入拟芥,通过多次筛选已经获得了转基因拟芥的纯合植株,对获得的转基因植株的表型分析发现,在过量表达at3g51960的转基因拟芥中对胁迫的抗性有一定程度的变化,因此推测at3g51960可能参与植物的抗性调节。

Later, in your country for a variety of reasons to split the Democratic Republic of Vietnam and the Republic of Viet Nam, the Government of South Vietnam behind China's civil war, as well as the backing of the United States occupation of the islands, such as the reasons for the Che-hung-xiu Island, south of the island, Dun-sandbank, Jinghong Island, South Island and amboyna and sent troops stationed.

在后来贵国由于种种原因分裂为越民主共和国以及越共和国,越政府利用中国内战以及背后美国撑腰等原因占领了沙群岛的鸿庥岛、子岛、敦谦沙洲、景宏岛、威岛和安波沙洲,并派军驻守。

Seismic energy is disturbed and attenuated seriously under the bad geological conditions for seismic exploration in southern Huanghai basin, and therefore the Meso-Paleozoic wave group of marine reflection seismic cannot be recognized and the seismogram does not meet the requirement for evaluation of pre-Tertiary oil/gas resources in southern Huanghai basin. In May 2006, marine magnetotelluric sounding experiment was carried out and reliable data were acquired.

针对黄海盆地深层地震地质条件差,地震波受屏蔽,能量严重衰减,在海洋反射地震资料中很难识别反映中-古生界的反射地震波组,不能满足黄海盆地前第三系油气资源前景评价的需要等问题,于2006年5月,开展了黄海海域海底大地电磁测深试验,在盆地内五莲斜坡和胶州凹陷区布设了3个海底大地电磁测深点,取得了可靠的海底观测数据。

According to literatures and visitings, we can see the formation of Maonan Minzu and the interaction between Maonan ethnic group and other ethnic groups. Take two villages which are located in Maonan mountainous area and county'seat for example, this paper explains the ethnic identity of Maonan Minzu.

本文将从人类学的视角,运用人类学的理论和方法,根据访谈和文献的记载来考察毛族的形成及其在历史上与他族群的互动状况,同时通过对毛聚居区的两个村落和所在自治县的县城进行田野调查,考察毛族族群认同的现状。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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