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Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域,用限制酶 Bfm 消化 PCR产物,10 %聚丙烯酰胺凝胶电泳检测。

Methods Colloidal gold-labeled monoclonal antiCK20 antibody was absorbed on nitrate cellulose membrane, and then the membrane was fixed on plastic strip with double face glue as checking line.

方法将经处理的CK20单抗标记在胶体金颗粒上并活性进行鉴定,合格后吸附在硝酸纤维膜上并固定在长方形硬质塑料胶片一端为测定线;同时将吸附有IgG蛋白胶体金的硝酸纤维膜固定在测定线的一侧为质控线。

The main results were as follows. The main nutrition bioactive components in Opuntia Milpa Alta were analyzed using chemical and instrumental analytical methods. The results showed that the content of crude protein and fat was 0.65%~0.68% and 0.30%~0.31% respectively. The total content of the amino acids was 6.81%~7.38%, and Glu was 1.08%~1.52%.The contents of pectin and cellulose was 1.47%~1.68%, 0.87%~1.22% respectively. They were oxalate free and rich in Ca 5.6%, Fe 8.06mg/100g, Zn 3.30mg/100g, VB6 5.62mg/100g, low in tannin 0.02%~0.04%.The bioactive matter content such as polysaccharide, polyphenol, and flavone were high, about 1.27%, 63.14mg/100g, 36.21mg/100g respectively.

主要研究内容如下:采用化学和仪器分析方法,对米邦塔仙人掌中的主要营养和生理活性成分进行分析,结果表明,粗蛋白含量为0.65%~0.68%,脂肪含量为0.30%~0.31%;氨基酸总量为6.81%~7.38%,其中谷氨酸含量达1.08%~1.52%;果胶、膳食纤维分别为1.47%~1.68%和0.87%~1.22%;干样钙含量5.6%,铁8.06mg/100g,锌3.30mg/100g;B族维生素丰富,其中干样VB6含量5.62mg/100g;单宁0.02%~0.04%;不含草酸;生理活性物质如粗多糖、多酚、黄酮类物质分别约为1.27%、63.14mg/100g、36.21mg/100g,半乳糖约占多糖中单糖总量的80%;HPLC检测出8种酚类物质,绿原酸含量最多达42.40μg/g,其次是芦丁和槲皮素,其含量分别为25.02μg/g和10.91μg/g。

Our results indicated that PPV-based nanofibers can be directly assembled into photoconductor devices using the simple and low-cost method.

此项研究展示了PPV基电纺纤维可以直接简便的组装成单根及阵列纤维模型器件,推进了具有光电特性的共轭高分子纳米材料的实用化进程。

Abstract] objective to improve the diagnosis of non-ossifying fiboma by analysing its x-ray feature.methods plain x-ray films was performed in all patients.x-ray finding in 8 cases with pathologically-confirmed non-ossifying were analyzed.results the clinical symptom was mild,the lesions occurred usually at the metaphysis of the long bones,the affected bones included femur(n=4),tibia(n=3),humerus(n=1).cortical type was seen in 5 cases,presenting as unilocular or multilacular transparent areas with in the cortex or tightly beneath the cortex,the lesion had a sclerotic margin.which was more obvious at the marrow side.medullary type was seen in 2 cases.the lesion was located at the center of the bone and grew centrally.the tumor was manifested as unilocular or multilocular.tramsparent area with sclerotic border and the bone cortex became thinner with slightly expanding on 11 sides.conclusion plain radiography is the elementally means to detect this disease.based on the typical x-ray signs of non-ossifying fibroma combined with clinical data,correct preoperative diagnosis can be made in most cases.

目的 探讨非骨化性纤维瘤的x线表现以提高诊断水平。方法所有病例均摄有x线平片,分析经病理证实的8例非骨化性纤维瘤。结果临床症状轻,好发于下肢长骨干骺端或骨干,股骨4例,胫骨3例,肱骨1例。皮质型5例,表现为皮质内或紧贴皮质下的单层或多层透亮区,病变向骨内发展进入髓腔,周围有致密硬化带环绕,以髓腔侧明显;髓质型2例,病灶在骨内中央发展,显示为单房或多房透亮区,边缘有硬化,骨皮质菲薄,轻微向周围膨隆。结论 x线为最基本的检查方法,典型病例x线平片结合临床症状即可明确诊断。

The tracheary elements in secondary xylems of Actaea asiatica root and rhizomes were observered with a scanning electron microscope and found to contain abundant types, which mainly included tracheids, tracheid-like vessels, fibriform vessels, and typical vessels. Of these types, the tracheids, tracheid-like vessels, fibriform vessels were first found. Scalariform perforation plates, reticulate perforation plates, mixed perforation plates and simple perforation plates were observed in the typical vessels and the reticulate perforation plates ,mixed perforation plates were first founded the lateral-wall perforation plates, multiple end walls and pit membrane vestige were also observed in the tracheary elements.

利用扫描电子显微镜对毛茛科类叶升麻根和根状茎次生木质部中的管状分子进行观察,发现其管状分子类型丰富,主要有:管胞、管胞状导管、纤维导管和典型的导管分子,其中管胞、管胞状导管和纤维导管为在该类群中首次报道;在导管分子中,存在着梯状穿孔板、网状穿孔板、混合型穿孔板和单穿孔板,其中网状穿孔板和混合型穿孔板为在该类群中的首次报道;对其导管分子上的侧壁穿孔板、多穿孔板和纹孔膜残余也进行了描述。

Using 3-year-old culms of 8 provenances of Bambusa chungii from Guangdong, Guangxi and Hainan Provinces, the indexes of wood properties, such as fiber dimensions and chemical composition were investigated and analyzed by the methods of Analysis of variance and correlation coefficient to reveal the geographic genetic variation situation.

以广东、广西和海南3个省8个产地的粉单竹群体3年生竹秆为研究材料,对其纤维形态、化学成分等纸浆材性指标进行测定,采用方差分析和相关分析方法对上述竹材性状进行遗传变异分析,以揭示粉单竹材性的地理遗传变异规律。

The results is two strains of bacteria Pseudomonas alcaligenes (No. 1203) and Cellulomonas (No. 1120) that could endure 60 times of the V water quality standard were selected, that is, 2 strains could grow in 6mg Pb /L and 60mg Cu /L media. Another 2 strains could endure 70 times of the standard were attained, Rhodococcus (No. 1314) and Acinetobacter (No. 1410), which could grow in 7mg Pb /L and 70mg Cu /L media.

共筛选出耐国家水质V类污水重金属Pb和Cu 60倍(Pb 6mg/L,Cu 60mg/L)的菌株共2株,1203号菌(产碱假单胞菌Pseudomonas alcaligenes)和1120号菌(纤维单胞菌Cellulomonas);耐V类重金属Pb和Cu 70倍(Pb 7mg/L,Cu 70mg/L)的菌株2株,1314号菌(红球菌属Rhodococcus),1410号菌(不动杆菌Acinetobacter)。

Composed of three orthogonal bundles of fibers and mat rix was proposed based on the assumption that the fiber bundle has the rectangular cross section.

根据三维正交机织复合材料的结构特点,在假设纤维束横截面为矩形的基础上建立一种单胞模型,该模型由 3组互相正交的纤维与基体组成。

As a new type of single-screw extrusion pulping equipment,the kneader-type pulp disperser has functions of extruding,mixing, shearing and so on,it can be used to treat the high yield pulp in order to decrease the shive content and improve the resultant paper physical properties.

纤维离解机,作为一种新型单螺旋挤压磨浆设备,它具有挤压、混合、剪切等功能,可用于处理高得率浆以减少纸浆中的纤维束含量和改善成纸性能,并可尝试用于麦草、芦苇等草类浆的处理以及高得率浆的过氧化氢漂白。

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Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事,那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来,随着部分预应力砼梁应用的推广,发现我国规范所采用的名义拉应力法的计算结果不稳定。

Gorky once said:"If it is not a book, I'd be stupid and indecent drowning."

高尔基曾说:"如果不是书籍的话,我就要被愚蠢和下流淹死。"