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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

General characteristics of cells, its chemical constituents ( protein, nucleic acid, carbohydrate, lipid), cell structure , cell cycle, cell division, four kinds of tissues (epithelial, connective, muscular, and nervous), organ, ten organ systems (dermal, skeletal, muscular, digestive, respiratory, circulatory, excretory, endosecretory, nervous, and reproductive).

细胞的一般特征,细胞的化学组成(蛋白质,核酸,糖类,脂类),细胞的结构,细胞周期,细胞分裂(无丝分裂,有丝分裂,减数分裂),四大组织(上皮组织,结缔组织,肌肉组织,神经组织),器官,十大系统(皮肤系统、骨骼系统、肌肉系统、消化系统、呼吸系统、循环系统、排泄系统、内分泌系统、神经系统和生殖系统)。

The blockade was given new impetus earlier this month when Manipur's government stopped Thuingaleng Muivah, the leader of the separatist group with which the government signed the ceasefire, from visiting the Manipuri village where he was born.

本月初曼尼普尔政府阻止与该国政府签署了停火协议的分裂组织领导人慕伊阿参观曼尼普尔他出生的村庄,这引起了附近曼尼普尔邦那加兰邦边境城镇Mao的暴力抗议。

In prophase, it localized on the microtubule organizing-center. In metaphase, it co-localized with α-tublin completely on spindle. In anaphase, it co-localized with α-tublin in aster and mid-zone. In telophase, it co-localized with α-tublin in cytoplasmic bridge connecting the two daughter cells.

在分裂前期,hSMP-1蛋白位于微管组织中心区域;在分裂中期,hSMP-1蛋白与组成纺锤体的微管呈完全共定位;在分裂后期,hSMP-1蛋白与星体和纺锤体中区的微管呈共定位关系;在分裂末期,hSMP-1蛋白则与两个子细胞之间的胞质桥完全共定位。

The government also regularly publicises threats by the Ogaden National Liberation Front, a Somali separatist group in the east, which has murdered foreigners and Ethiopians exploring for oil in that area.

政府还定期宣传奥加登民族解放阵线(Ogaden National Liberation Front)制造的威胁,这是东部索马里分裂组织,曾杀害了众多外国人以及到该地区从事石油勘探的埃塞俄比亚人。

Police in Northern Ireland say they have two suspects in custody in the case of a policeman killed last night in an attack claimed by an IRA splinter group.

北爱尔兰的警察说在昨晚由爱尔兰共和军一个分裂组织制造的袭击中,拘留了两名枪杀警察案的嫌疑人。

Police in Northern Ireland say they have two suspects in custody in the case of a policeman killed last night in an attack claimed by an IRA splinter group.

这两名犯罪嫌疑人被认为与前日发生的据称由爱尔兰共和军分裂组织策划的警察被杀事件有关。

Subsequently, Air Marshall Perence Shiri—formerly commander of the North Korean-trained Fifth Brigade, used by the Mugabe government to crush so-called Ndebele dissidents in the west of the country in the early 1980s—became involved in mining at Marange.

随后,空军上将普兰斯什里—之前是在北韩受训的第五旅的司令,该旅是穆加贝政府于上世纪80年代初期为粉碎国家西部所谓的恩德比利分裂组织—也参与马兰吉地区的采矿活动。

This week the government brushed off allegations of war crimes in the Ogaden region, where it is conducting a ferocious counter-insurgency against an armed separatist group, the Ogaden National Liberation Front.

这周,政府洗清了其面临的欧加登地区战争罪的指控,在该地区,政府正对一武装分裂组织——欧加登国民解放战线残酷清剿。

This week the government brushed off allegations of war crimes in the Ogaden region, where it is conducting a ferocious counter-insurgency against an armed separatist group, the Ogaden National Liberation Front.

目前,埃政府正在东部奥加登地区猛烈围剿那里的一个武装分裂组织——奥加登民族解放阵线。本周,埃政府遭指责,称该行动无异于是犯下了战争罪,但埃政府对此矢口否认。

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