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分光光度测定法

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Methods The activities of liver microsomal erythromycin N-demethylaseand aminopyrine N-demethylase were measured by spectrophotometry. The levels of mRNA expressions of CYP3A1, CYP1A2, CYP2E1, mdrla and mdrlb in hepatic microsome were determined with RT-PCR method.

用分光光度法测定大鼠肝微粒体红霉素N-脱甲基酶、氨基比林N-脱甲基酶的活性,用逆转录聚合酶链反应技术测定大鼠肝脏CYP3A1、CYP1A2、CYP2E1、mdr la和mdr lb基因的水平。

A flow injection spectrophotometry is proposed for simultaneous determination of iron ion andiron ion(Ⅲ based on o-phenanthroline spectrophotometry,using double zone injection and a reduction column packed with cadmium plated zinc power.

将流动注射技术引入邻菲罗啉分光光度法测定Fe2+分析体系,采用单阀双带镀镉锌片还原柱带隔离的阀体流路,建立了同时测定微量Fe2+和Fe3+的分析方法。

With the development of science and technology, sensitive, rapid, reproducible, simple and accurate analytical methods are required for the determination of trace elements in geological, biological and environmental samples. The direct determination of extremely low concentration of trace element with modern atomic spectroscopic method, such as atomic absorption spectrometry is so difficult. On one hand, the limitations are associated not only with the insufficient sensitivity of these techniques but also with matrix interference.

随着科学技术技术的发展,在进行地质、环境、生物等样品中痕量金属元素的测定时,常常要求达到ppb级的水平,虽然火焰原子吸收分光光度法具有很高的灵敏度,但是要直接测定这些样品中的痕量组分却很难,这是因为,一方面,样品本身的物理化学状态有的不适合直接测定,或组分的含量低于分析方法的灵敏度;另一方面是存在基体干扰。

When high iodate potassium spectro-photometric method is used to carry on the determination to manganese concentration, calcium ion can be interferenced in the determination.

电解锰废水中,钙往往与锰共存,采用高碘酸钾分光光度法(GB/T 11906-1989)对锰浓度进行测定时,钙离子存在对锰测定的干扰较大,从而影响测定结果。

The method of using formaldehyde buffer solution a b sorption-hydrochloric pararosaniline spectrophotometry to determine sulfur diox ide in air has higher sensitivity and good selectivity,and prevents from adoptin g mercurial absorbent.But the conditions in lab are not well controlled,the v alue of reagent blank and standard curvilinear slope can't accord with the one s tipulated in"Methods for Air and Waste Gas Monitoring and Analysis".Some opra tional techniques for SO2 determination in lab are introduced for reference.

文摘:以甲醛缓冲溶液吸收-盐酸副玫瑰苯胺分光光度法测定空气中的二氧化硫,方法灵敏度高,选择性好,避免了使用含汞的吸收液,但如果实验条件控制不好,试剂空白值及标准曲线的斜率就会不符合《空气和废气监测分析方法》中规定的值,本文着重从操作技巧方面对二气化硫测定的实验室质量控制进行分析,仅供参考。

The optimum experimental condition for simultaneous UVspectrophotometric determination of the contents of aspirin, phenacetin and caffeine in aspirin compound tablets and the basic principle

本文研究了复方阿斯匹林片中阿斯匹林、那西丁和咖啡因含量同时测定的紫外分光光度法的最佳实验条件,并简述偏最小二乘法多组分同时测定中的基本原理和应用。

The content of total flavonoids and tannin in common purslane was determined with ethanol extraction and spectrophotometry method.

方法]采用乙醇浸提法测定马齿苋中类黄酮含量,并用分光光度法测定马齿苋中单宁的含量。

A new method for determination of oleanolic acid in chinese quince by thin layer chromatography-spectrophotometry was established.

建立测定木瓜中齐墩果酸的薄层色谱-分光光度法的新方法,用薄层色谱分离杂质,优化了分析条件,讨论了影响测定的因素。

The experimental procedure were predigested under the condition that did not effect the experimental results by using alkal potassium persulphate oxidation -ultraviolet spectrophotometry to determine the total nitrogen in water sample with first water instead of redistillation non-ammonia water.

经实验证明,用碱性过硫酸钾氧化-紫外分光光度法测定水样中的总N,以一次蒸馏水代替重蒸馏无氨水,既减少了实验操作程序,又不影响测定结果,同时本文确定了最佳实验条件,并对标准样品进行分析,结果理想。

Activity of calcium and sodium pump, parameters of erythrocyte injury, level of calcium in erythrocyte and platelet count and function were obtained during the study.

采用原子吸收分光光度法测定红细胞钙,并同步测定钙泵、钠泵活性,以及红细胞损伤和血小板功能、数量的有关指标。

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