内皮细胞

Results Many endothelial cells were observed with higher vigor and adherance to the wall after incubation for 3-5h, and spreaded to form cell masses for 12-24h, and grew densely and converged into single layer which characteristically arranged to cobble-stone shape after incubation for 1-2 weeks.

结果 原代细胞培养3~5h后，大部分活力较高的内皮细胞已开始贴壁，12~24h后，内皮细胞己铺展形成由数个细胞组成的细胞群，1~2w后，细胞呈生长密集状并汇合形成单层，在倒置相差显微镜下，呈特征性"鹅卵石状"或"铺路石状"镶嵌排列生长。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

The entire process of PMN rolling on, adhering to, and transmigrating across the EC monolayer was recorded under both phase-contrast and fluorescence optics.

我们发现当单一PMN开始穿过内皮细胞层，会引发紧邻的内皮细胞 [Ca2+]i上升，但对其附近未直接接触此一PMN的内皮细胞，则没有影响。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberines function may be downregulating the expression of ICAM-1 on the HUVEC .

结果：小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附，其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达；小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附，对淋巴细胞表面粘附分子CD18的表达无影响。

This study investigated endothelial chimerism and its influential factors with X and Y chromosome probes by fluorescence in situ hybridization.

使用X，Y染色体探针通过荧光原位杂交法观察移植肾中血管内皮细胞被受体内皮细胞替代的情况，并分析内皮细胞替代的影响因素。

The rationals of anti-angiogenesis are the following: the therapeutic target is tumor endothelial cells which are more genetically stable, more homogeneous, and with lower mutation rate; the anti-angiogenesis drugs can contact endothelial cells directly, with higher potency and lower drug barriers and side effects; the aim of anti-angiogenesis therapy is to block the oxygen and nutrition supply from vessels, instead of killing tumor r ells per se; the aim of anti-angiogenesis is to reduce tumor size instead of eradicate tumor which are different from traditional chemotherapy.

使用抗血管新生疗法根据的理论基础为以肿瘤血管的内皮细胞为治疗药物的攻击标的；肿瘤血管的内皮细胞基因比较稳定、同质性高、而且突变率低；投与的药物可以直接接触内皮细胞，减少投与药物屏障，因此增加有效投与药物的能力及减少药物副作用；以截断供应肿瘤氧气养分的血管为目标：以减小肿瘤的体积大小为目的，与传统化学疗法以根除肿瘤的目标不同。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberine's function may be downregulating the expression of ICAM-1 on the HUVEC .

结果：小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附，其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达；小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附，对淋巴细胞表面粘附分子CD18的表达无影响。

The thoracic duct endothelia in vitro could remain their biological properties, and could be made a tool for studing their functions and cancer metastasis mechanism by lymphatics. The positive expression of VEGF-C in cancer cells was correlated with cancer development, it seem to be that VEGF-C induce lymphoangiogenesis through its receptor Flt-4. The Flt-4 expression of lymphatics in cancer tissues was assosiated with cancer development, it is considered that there are new lymphatics in cancer tissues.

体外培养的大鼠胸导管内皮细胞可以保持体内淋巴管内皮细胞的生物学特性，可作为研究大鼠淋巴管内皮细胞功能和进一步研究癌转移机制的体外实验材料；大鼠大肠癌组织腺体的VEGF-C表达与肿瘤进展有关，推测其通过受体Flt-4诱导淋巴管生成；Flt-4在淋巴管的阳性表达随肿瘤进展增多，推测在癌组织内存在淋巴管的新生。

Methods The rat thoracic duct endothelia were cultured and the morphology observed and identified by TEM and Factor Ⅷ-related antigen immunohistochemically. Wistar rats were used to make the animal model of primary colorectal cancer, the cancer type were appraised by HE staining and PAS Reaction. The expression of VEGF-C and its receptor Flt-4 and ICAM-1 in the cancer tissues were carried through immunohistochemistry staining and In situ hybridization.

通过体外培养大鼠胸导管内皮细胞和构建大鼠原发性大肠癌动物模型，观察内皮细胞的形态和生长状态，用透射电镜和Ⅷ因子相关抗原进行鉴定，用HE染色和过碘酸雪夫氏反应区别癌组织的类型和期别，用免疫组织化学方法和原位杂交方法研究内皮细胞和大肠癌组织的VEGF-C及其受体Flt-4和ICAM-1的表达。

He knows that he's one of the mostdominant players in the NBA right now.

我知道他现在是联盟里最有统治力的球员之一了。

Using analysis method of BCG , the dissertation analyzed the management of voluntary service organizations .

利用BCG分析方法对我国的志愿服务组织管理进行了分析。