内皮细胞

Different measures of potentill anserine extract has different effects on endothelial cell of capillary vessel of small rats' alveolar wall. Endothelial cell of capillary vessel of alveolar wall has hyperplasia and hypertrophy in different degrees; it leads to permeability of capillary vessel increasing in different extent. With the situation, different concentration of potentill anserine extract promotes vascular endothelial cell factor of small rat's pulmonary tissue secretion.

不同剂量的蕨麻提取物对小鼠肺泡壁毛细血管内皮细胞的影响不同，肺泡壁毛细血管内皮细胞呈不同程度的增生肥大导致毛细血管通透性呈现不同程度的增加，表明可能和不同浓度的蕨麻提取物促进小鼠肺组织某些血管内皮细胞因子（Vascular endothelial cell factor VECF ）的分泌功能有关。

The small rat pulmonary tissue surface of ⅲ and ⅳ group can't see any abnormality; the capillary vessel of alveolar wall dilation appears a little hyperemia in different degree under the microscope.conclusion different measures of potentill anserine extract has different effects on endothelial cell of capillary vessel of small rats' alveolar wall. endothelial cell of capillary vessel of alveolar wall has hyperplasia and hypertrophy in different degrees; it leads to permeability of capillary vessel increasing in different extent.

不同剂量的蕨麻提取物对小鼠肺泡壁毛细血管内皮细胞的影响不同，肺泡壁毛细血管内皮细胞呈不同程度的增生肥大导致毛细血管通透性呈现不同程度的增加，表明可能与不同浓度的蕨麻提取物促进小鼠肺组织某些血管内皮细胞因子（vascular endothelial cell factor vecf ）的分泌作用有关。

METHODS: Ahmed glaucoma valves were implanted in 34 eyes of 34 patients with refractory glaucoma. Density and morphology of corneal endothelial cells were evaluated in the superotemporal, superonasal, infratemporal, infranasal, and central corneal areas to calculate mean value of total cornea before and at 3 and 6 months after surgery. Percentage of hexagonal cells was statistically summarized.

在34例(34眼)疑难青光眼患者眼球颞上象限行Ahmed青光眼阀植入，移植前及移植后3，6个月对角膜中央、颞上、颞下、鼻上及鼻下区内皮摄像后测定分析，得出角膜内皮细胞密度，并取全角膜平均值，同时观察角膜内皮细胞形态变化，统计六角形细胞所占比例。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

These indicated that endothelial cells of the mural coronary artery are free from atherosclerosis due to higher shear stress caused by myocardial bridge. The intima of the section proximal to mural coronary artery was likely to be sclerosed due to the lower and oscillatory shear force.

提示 壁冠状动脉内皮细胞受到心肌桥压迫导致血流切变力增高产生适应性反应，对内皮细胞有保护作用，而其近段内皮细胞由于切变力较低容易被损伤，成为动脉粥样硬化发生的基础。

NO may be one of the important molecular regulators to permeability of strial capillary endothelial cells by TNF-α,and changes of F-actin content probably relate to the regulation in these cells.

TNF-α能增加耳蜗血管纹微血管内皮细胞的通透性，NO可能是TNF-α调控内皮细胞通透性的重要分子机制之一；耳蜗微血管内皮细胞中F-acfin含量的改变可能与其通透性的调控有关。

Methods Strial capillary endothelial cells in guinea pig cochlea was dissociated and cuhureed to establish a model for its permeability in vitro.,The animals were divided into TNF-α,TNF-α+L-arginine,TNF-α+NG-minomethyl-L-arginine and control groups.

体外分离培养豚鼠耳蜗血管纹微血管内皮细胞并建立内皮细胞通透性体外模型，观察TNF-α、TNF-α连同L-精氨酸(L-arginine，L-Arg)或L-单甲基精氨酸(NG-monomethyl-L-arginine，L-NMMA)对内皮细胞通透性的影响。

When cultured in three-dimensional matrix, the cells were elongated and migrated into the gel, with a phenotype similar to that in the process of angiogenesis and vasculogenesis in vivo .

在二维平面培养中，原代心脏血管内皮细胞呈铺路石样排列；而在三维胶原培养模式中，内皮细胞呈长梭状形态，并迁入胶原培养介质中，和体内血管新生及血管生成过程中的内皮细胞活化表型相似。

ET and NO are the biology regulatory factors produced by vascular endothelial cell.ET is a peptide with vasoconstrictive and vasodilator effect produced by endothelial cells,and NO as a powerful endothelium-derived relaxing factor,has a strong role in dilation of blood vessels.

ET和NO是血管内皮细胞产生的生物调节因子，ET是由内皮细胞产生的一种多肽，具有缩血管和扩血管作用，而NO为一种强有力的血管内皮细胞舒张因子，具有强烈扩张血管作用。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。