共生物

This is an early biophysical articles, a total of 38, contribute to the biophysical structure of neural networks and neurons understanding.

详细说明：这是一篇早期的生物物理的文章，共38页，有助于对生物物理中的神经网络结构和神经元的理解。

Another interesting phenomenon is that when CaLP and p21 are co-transfected, the expression of p21 can stimulate CaLP translocate from cytoplasm to nucleus. By using methods of bioinformatics, point mutation and flow cytometry, we confirmed the importance of Y139 in the nuclear-cytoplasmic trafficking of CaLP.

激光共聚焦实验还发现当p21过表达时能够引起CaLP从细胞质向细胞核的转移，通过生物信息学方法，免疫共沉淀实验和流式细胞分析表明，CaLP的139位酪氨酸残基存在着较高的磷酸化潜力且在CaLP的核质穿梭和蛋白相互作用中起到了重要的作用。

As they are evenly dispersed throughout genomes, usually characterized as codominant and highly polymorphic systems amenable to genotyping by polymerase chain reaction, and generally inherited in a Mendelian fashion, microsatellites have become instrumental as genetic markers in many areas.

摘 要：微卫星标记广泛分布于原核生物和真核生物基因组中，具有密度大、多态性丰富、遵循孟德尔分离定律共显性遗传、易于PCR扩增等特点，已成为近年来最引人注目的新型DNA标记。

The thesis consists of six chapters. In the first, the technologies of atomic force microscopy and of the measurement elasticity of biomolecules were introduced. In the second, the validity of VSPFM was confirmed by lift mode atomic force microscopy. In this chapter, the height of DNA was measured by lift mode atomic force microscopy, which demonstrated that the method of height measurement of biomolecules by VSPFM was correct and established the foundation of the method of measurement elasticity of biomolecules by vibrating mode scanning polarization force microscopy. In the third chapter, detailed work has been illustrated on the foundation of the method of measurement elasticity of biomolecules by VSPFM. And the compressive elasticity of DNA was measured. In fourth and fifth chapters, the method was applied in the measurement elasticity of proteins. Two proteins elasticity, fibre-like protein α-synuclein and global protein IgG, were measured by VSPFM, through which the method wound its way to the application of biomolecules. In last chapter, the final part of the thesis was a summary. A conclusion of the thesis and a self-comment on my work as a PhD candidate have been made, and expectation about the further works has been addressed.

本论文共分为六章，第一章，引言部分主要介绍了原子力显微镜技术及生物大分子弹性测量技术；第二章主要是VSPFM方法的正确性论证，介绍抬高模式原理，利用抬高模式原子力显微镜对DNA的高度进行测量，论证振动模式扫描极化力显微镜测量生物大分子的高度的正确性以及准确性，从而为振动模式扫描极化力显微镜测量生物大分子的弹性方法的建立奠定基础；第三章以脱氧核糖核酸为例详细介绍了振动模式极化力显微镜测量生物大分子弹性的方法的建立，对DNA的压弹性进行了初步的测量和分析；第四章和第五章介绍了振动模式扫描极化力显微镜在蛋白质弹性测量中的应用：α-synuclein和IgG分别是纤维状蛋白和球状颗粒蛋白，通过振动模式扫描极化力显微镜测量这两种蛋白质的弹性，摸索振动模式扫描极化力显微镜在蛋白质弹性测量中的应用；第六章对全文进行了总结，在对论文的工作进行归纳和自我评价之后，还对进一步的工作进行了展望。

With the development of biotechnique, medical and computer technique, and the changes of life, value, aesthetic, social and marriage concept, the phenomenon of "third gender" will express itself in coexistence of diverse constituents: namely, the coexistence of mainstream and branches, unitarity and multiplicity, tradition and modern, virtuality and reality, biological and psychological behaviors, etc.

未来趋势受生物技术、医药技术、计算机技术、纳米技术及生命观、价值观、审美观和心理定位、社会角色、婚姻家庭、环境因素的影响，具有主流和支流、传统和时新、单一和多样、渐变和突变、现实和虚拟、有性和无性、生物和心理、生物和社会共存共融的形态。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

On the base of summering the treatment technique of starch wastewater,the treatment technique of high concentration starch wastewater and application of carriers in the biotreatment wastewater were reviewed. Chapter 2:Effect of Latex carrier in biotreatment of high concentration starch wastewater.

本文共分四章：第一章淀粉废水生物处理技术及填料的研究进展总结淀粉废水处理技术的基础上，重点综述了高浓度淀粉废水生物处理技术及填料在生物法处理废水中的应用进展。

In this paper, the stable Au complexes are classified according to the coordination atoms and their characteristic structure. The recent development of the researches on the antitumor activity of Au complexes including the structure-activity relationship, biological targets and the mechanisms of action are reviewed. The structure of the coordination ligand and the leaving group have great impact on the in vitro cytotoxicity of the Au complex. Many physical and biological methods are introduced to study the interaction between the Au complexes and the suspected bio-target, such as DNA, protein, mitochondrion, thio-containing biomolecules, etc. The interaction modes (intercalating, electrostatic interaction, covalent bonding, etc.) are focused to illustrate the reason for the antitumor activity of Au complexes.

本文按配位原子的不同总结了稳定三价金配合物的结构特征，按其生物活性的构效关系、生物靶点和作用机制综述了三价金配合物抗肿瘤活性研究的最新成果：配体的结构特点以及离去基团对三价金配合物的体外细胞毒性影响较大；介绍了用于检测三价金配合物与可能的生物靶分子之间的相互作用的多种物理和生物学方法，重点关注了相互作用的模式，如嵌入/静电吸引/共价结合等，并解释了三价金配合物抗肿瘤活性的原因。

In this paper, the stable Au complexes are classified according to the coordination atoms and their structural characteristics. The recent development of the researches on the antitumor activity of Au complexes including the structure-activity relationships, biological targets and the mechanisms of action are reviewed. The structure of the coordination ligand and the leaving group have great impact on the in vitro cytotoxicity of the Au complex. Many physical and biological methods are introduced to study the interaction between the Au complexe and the suspected bio-target, such as DNA, protein, mitochondrion, thio-containing biomolecules, etc. The interaction modes (intercalating, electrostatic interaction, covalent bonding, etc.) are focused to illustrate the reason for the antitumor activity of Au complexes.

本文按配位原子的不同总结了稳定三价金配合物的结构特征，按其生物活性的构效关系、生物靶点和作用机制综述了三价金配合物抗肿瘤活性研究的最新成果：配体的结构特点以及离去基团对三价金配合物的体外细胞毒性影响较大；介绍了用于检测三价金配合物与可能的生物靶分子之间相互作用的多种物理和生物学方法，重点关注了相互作用的模式，如嵌入/静电吸引/共价结合等，并解释了三价金配合物抗肿瘤活性的原因。

To study the effects on the body of animals after incorporation of 3H-TdR and its mechanism, the laws of distribution A retention and absorbed dose in blood , thymus, spleen, femur, heart, lung,kidney, liver, brain , spermary , small intestine and muscle were detected by liquid scintillation counter after 3H-TdR was injected into the tail vein of mouse; the process of dynamic incorporation of in the lymphocytic cell nucleus of blood , thymus , spleen and the nucleus of femur was monitored; the absorbed dose in the cell nucleus was estimated; the influence to RNA"s synthesizing and cell"s survival was observed using the technology of CLSM; A segment of Egr-1 gene was cloned with reverse transcriptase- polymerase chained reaction, the influence to the expression of Egr-1 gene in the spleen cells was observed.

为了探讨有机结合氚内污染对生物机体的影响及其作用机理，本课题应用均相液体闪烁测量技术研究了氚标记胸腺嘧啶核苷(~3H-TdR)经尾静脉注入小鼠机体后，在血液、脑、胸腺、心脏、肺脏、肝脏、脾脏、肾脏、小肠、睾丸、肌肉和股骨共12种组织器官的生物动力学分布规律及其吸收剂量；应用纸片法液体闪烁测量技术研究了~3H-TdR内污染在外周血、胸腺和脾脏淋巴细胞及骨髓细胞的动态滞留规律，估算了细胞核的吸收剂量；应用共聚焦显微镜观察了~3H-TdR内污染对脾细胞核酸合成及其存活率的作用，应用反转录PCR技术研究了~3H-TdR内污染对脾细胞早期生长反应基因-1(Egr-1)转录表达的影响。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。