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兔子

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The novel Rabbit Redux is the second one of John Updikes literary masterpiece, the Rabbit quartet.

兔子归来》是约翰·厄普代克的代表作"兔子四部曲"中的第二部。

The zoology balance simulator of rabbit and fox ,which can simulat...

兔子狐狸生态平衡模拟器,可以模拟兔子狐狸相互依存的状况。

Written by James Thurber Within the memory of the youngest child there was a family of rabbits who lived near 1a pack of wolves.

肇事的兔子在最小的孩子还记得的那个时候,在狼群的附近居住着兔子一家。

For at least two hours the Boy loved him, and then Aunts and Uncles came to dinner, and there was a great rustling of tissue paper and unwrapping of parcels, and in the excitement of looking at all the new presents the Velveteen Rabbit was forgotten.

兔子是所有在一切中最好的。但它最多只被孩子喜爱了两个小时,接着婶婶和叔叔来吃午饭了,他们又带来了新的礼物,于是绒布兔子就被忘记了。

The Rabbit Welfare Fund recommends one and a half to two and a half cups of fresh vegetables per day.

兔子福利基金The Rabbit Welfare Fund建议每天提供一杯半至两杯半的新鲜蔬菜给兔子

The immunoglobulin IgY and IgG was purified from the yelk of egg and serum of rabbit, respectively.

用分离物HF-WX提纯物免疫母鸡和兔子,分别从母鸡蛋卵黄和兔子抗血清中提取免疫球蛋白IgY和IgG。

Results indicated that the anaplasma could be observed by microscope in erythrocyte of experimental animals at 6th to 8th day, and the anaplasma reached peak number in erythrocyte at 16th to 20th day, disappearing at 25th day after infection.

结果表明,无浆体在小鼠和兔子体内呈现一定的规律性。无浆体在感染后第6d和第8d分别在小鼠和兔子血液中可以检测到;感染后第16d~19d和第17d~20d在两者体内达到感染高峰;感染后第20d和第25d均逐渐消失。

Rabbit in the trees and desperate not to stand,all of asudden fell down from the positive three dogs,rabbits opportunity and ran away.

兔子在树上,仓惶中没有站稳,一下子掉了下来,砸晕了正仰头看的三只猎狗,兔子乘机逃跑了。

Disney Characters A timeline of character debuts Before Mickey 1924 Alice (of The Alice Comedies, a series of 56 silent cartoons) 1927 Oswald the Lucky Rabbit (star of 26 silent cartoons) Recurring Characters: Mickey and Friends 1928 Mickey Mouse and Minnie Mouse, in Steamboat Willie 1929 Peg-Leg Pete, in The Barn Dance 1929 Clarabelle Cow and Horace Horsecollar, in The Plow Boy 1930 Pluto (AKA "Rover"), in The Chain Gang 1932 Goofy (AKA "Dippy Dawg,""George Geef"), in Mickey's Revue 1934 Donald Fauntleroy Duck, in The Wise Little Hen 1937 Huey, Dewey, and Louie Duck, in the Donald Duck Sunday comics 1937 Daisy Duck (AKA "Donna Duck"), in Don Donald 1943 Chip 'n' Dale, in Private Pluto Stars of Disney Feature Films 1937 Snow White, in Snow White and the Seven Dwarfs 1940 Pinocchio and Jiminy Cricket, in Pinocchio 1941 Dumbo, in Dumbo 1942 Bambi, in Bambi 1950 Cinderella, in Cinderella 1951 Alice and the White Rabbit, in Alice in Wonderland 1953 Peter Pan and Tinkerbell, in Peter Pan 1955 Lady and Tramp, in Lady and the Tramp 1959 Aurora, in Sleeping Beauty 1961 Pongo, Perdita, and the pups, in 101 Dalmatians 1967 Mowgli and Baloo, in The Jungle Book 1974 Robin Hood and Maid Marian, in Robin Hood 1986 Basil, in The Great Mouse Detective 1989 Ariel, in The Little Mermaid 1991 Belle and Beast, in Beauty and the Beast 1992 Jasmine and Aladdin, in Aladdin 1994 Simba, Timon, and Pumbaa, in The Lion King 1995 Buzz Lightyear and Woody, in Toy Story 1995 Pocahontas, in Pocahontas 1996 Esmerelda, in The Hunchback of Notre Dame 1998 Hercules and Megara, in Hercules 1998 Mulan and Mushu, in Mulan 2001 Milo Thatch, in Atlantis: The Lost Empire 2001 Lilo and Stitch, in Lilo And Stitch 2002 Sulley, in Monsters, Inc.

狄斯耐人物一个个性初次登场的时间期限在米奇之前 1924 爱丽丝(爱丽丝喜剧,一系列的 56幅沈默的卡通) 1927 奥斯瓦德幸运的兔子(26幅沈默的卡通的星)回到个性:米奇和朋友 1928 米老鼠和米妮老鼠,在汽船威利 1929 钉-腿的彼得,在谷仓跳舞方面 1929 Clarabelle 母牛和贺瑞斯 Horsecollar,在犁男孩中 1930 冥王星,在链组织中 1932 古菲(AKA&脑筋不正常的 Dawg,&&乔治 Geef&),在米奇的讽刺时事的滑稽剧 1934 唐纳德 Fauntleroy 鸭子,在明智的小母鸡中 1937 Huey ,杜威和中尉鸭子,在唐纳德鸭子星期日连环图画中 1937 黛西没入水中(AKA&堂娜鸭子&),在君唐纳德 1943 削'n'山谷,在私人的冥王星狄斯耐特征电影的星 1937 下雪水于白色,在雪白色的和七个矮子 1940 童话中的主角和表示温和的惊讶蟋蟀,在童话中的主角 1941 Dumbo,在 Dumbo 1942 Bambi,在 Bambi 1950 灰姑娘,在灰姑娘 1951 爱丽丝和白色的兔子,在奇境的爱丽丝 1953 彼得平锅和 Tinkerbell,在彼得平锅 1955 淑女和重步声,在淑女和重步声中 1959 奥罗拉,在睡着的美人 1961 在 101只大麦町中的 Pongo , Perdita,和学生 1967 Mowgli 和 Baloo,在丛林中订购 1974 罗宾头巾和少女圣母玛利亚崇拜者,在罗宾头巾 1986 罗勒,在伟大的老鼠侦探中 1989 瞪羚,在小的美人鱼中 1991 美女和畜牲,在美人和畜牲中 1992 茉莉属植物和安拉丁,在安拉丁 1994 Simba , Timon 和 Pumbaa,在狮子星国王中 1995 发出嗡嗡声 Lightyear 和多树木的,在供玩耍的故事中 1995 风中奇缘,在风中奇缘 1996 Esmerelda,在 Notre 夫人的驼背中 1998 大力英雄和 Megara,在大力英雄 1998 Mulan 和 Mushu,在 Mulan 2001 Milo 盖屋顶的材料,在阿特兰提斯:失去的帝国 2001 Lilo 和一针,在 Lilo 和一针 2002 Sulley,在怪物,公司 2003 奈莫舰长,在发现奈莫舰长方面 2004 幼鲑,在难以置信人中

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达,且在未成熟卵母细胞中,MⅠ期的表达明显强于GV期的表达;②RT-PCR产物克隆测序结果显示:昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp~1153bp)]完全一致;Blastn比对结果发现:在不同物种中差异较小,选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析,提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%,100%,100%,36%,100%,100%,67%,100%,100%,92%,99%,99%,99%;Clustal 1.81和Treeview构建出的分子进化树表明:在上述14种生物中昆明小鼠与中国仓鼠进化关系最近,与兔子、猪聚成一簇,与食蟹猴进化关系最远;③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段,重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

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