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光度计

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Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果:提取液主要为Ⅰ型胶原蛋白;72小时37℃恒温培养箱内培养无细菌生长;紫外分光光度计测定,吸收高峰为230nm;氨基酸成分主要为甘氨酸:占40%,脯氨酸:14.3%。显微镜下见:相互交织的胶原原纤维结构组成的网状结构;纤维均匀,有规则横纹。胶原蛋白液在37℃恒温下,可制成凝胶,用于培养细胞;用磷酸液冲洗胶原蛋白凝胶可成固体片状,制成胶原膜,用作医用材料。

Methods BSA incubated withglucose were subjected to PAGE and SDS-PAGE,fluorescence spectrum and fluorescence intensity were measured using fluorometer.

牛血清白蛋白与葡萄糖在体外共同孵育后,经聚丙烯酰胺凝胶电泳及荧光分光光度计测定荧光值和扫描荧光光谱。

The chemosensitivity, apoptosis and caspase3 activity of K562 and K562/A02 cells were evaluated by MTT assay, flow cytometer and fluorometer respectively.

以MTT、流式细胞术和荧光分光光度计观察RPL6对化疗药物耐药性、凋亡和caspase3的作用。

The chemosensitivity, apoptosis and caspase??3 activity of K562 and K562/A02 cells were evaluated by MTT assay, flow cytometer and fluorometer respectively.

以MTT、流式细胞术和荧光分光光度计观察RPL6对化疗药物耐药性、凋亡和caspase??

Regular porosity in about 38 nanometer size was observed using atomic force microscopy. Excitation and Emission spectrum were obtained by using F-4500 fluorometer.

用F-4500型荧光分光光度计测量其三维荧光谱,与未经氧化的铝片作对比,发现前者的激发谱在280 nm~340 nm有一个较宽的谱带,并且在253.8 nm附近有一个肩峰,发射谱有一个以417.4 nm为峰值的较宽谱带,而后者却无任何明显的发射峰和激发峰。

Based on the different permeability of DNA-intercalant dyes YO-PRO-1 and propidium iodide to the membrane of viable, apoptotic and necrotic cells, cell samples were stained with 4μmol/L YP and 4μg/ml PI for 10 min, and the fluorescence intensity of both YP and PI were measured by fluorometer at Ex/Em wavelength of 485/538nm and 530/590nm, respectively.

根据正常细胞、凋亡细胞和坏死细胞的细胞膜对核酸荧光染料的不同选择通透性,用4μmol/LYO-PRO-1和4μg/ml碘化丙啶(Propidiumiodide,PI)染色96孔板中的细胞样品。分别在485/538和530/590的检测波长下借助荧光分光光度计检测细胞样品孔的YP和PI荧光强度。

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生产、代理产品如下:硬度计无损检测仪器三坐标测量机显微镜其他量仪疲劳试验机放大镜拉力试验机台秤万能试验机弯曲试验机其他专用仪器仪表冲击试验机恒温试验设备高度计天平仪器计价秤强度试验机其他分析仪器信号发生器温度校验仪表地上衡其他试验机非金属材料试验机投影机百分表游标卡尺腐蚀试验箱包装件试验机其他实验仪器装置色谱仪电导仪器速度测量仪表光谱仪、光度计测力仪表测厚仪压力计色标、色卡 LCR测量仪真空测量仪器汽车试验设备非接触式温度计其他光学仪器千分尺压力式温度计定量秤其他电子产品制造设备工具量具产品广陆系列产品威海量具系列产品新锡量、锡工、申韩系列产品联思系列产品青海量具系列产品上工系列产品上量系列产品成量系列产品哈量系列产品桂量、杭工量系列产品西南系列产品大阳、ASIA、日本三丰量具、日本孔雀、系列产品申工、塞尺、螺纹样板、半径规、钢直尺水平仪、磁性工具系列产品钻夹头、接杆、丝锥夹头平板架子垫铁、曲铮、铸铁方箱、比测台、V型铁、强力磁铁、取出器、圆规、角度规、大力钳、针规铁皮剪刀、切割片、平板|环规、塞规、螺旋丝锥、挤压丝锥、左牙丝锥加长直柄钻、加长直柄钻美标钻、白直钻哈钻、青钻、1/2柄钻、锥柄加长钻、欢迎来电咨询。

The X-ray images and spectra obtained usingChandra's High Energy Transmission Grating Spectrometer show that astrong wind is being driven away from the center of NGC 1068 at a rateof about a million miles per hour.

通过钱德拉的高能传送光栅光度计所获得的 X 射线图像和光谱显示一股强风被正从 NGC 中心以每小时一百万英里的速度喷射而出。

All animal was undertaken resuscitation for 5 hours.Then we observed histopathology change of lung、hepar and kidney,detected blood gas analysis and functional parameter of hepar and kidney,and detected intestine and mesentery vascular permeability.

各组实验动物行液体复苏5小时后,观察肺、肝、肾组织病理学改变,检测血气分析及肝、肾相关功能指标,采取分光光度计测出小肠和肠系膜组织的血管渗透性等。

To study the method of hydride generation atomic fluorescence spectrometry in determining the content of Total arsenic in food.

目的 应用AFS -2 3 0a型原子荧光光度计进行氢化物发生原子荧光法测定食品中总砷的研究。

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