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Activities of Aminopyrine N-demethylase, Ethoxycoumarin O-deethylase and Glutathione S-transferase in the hepatopancreas and gills of Fenneropenaeus chinensis were measured by UV spectrophotometry and fluorospectrophotometry. The effects on the activities of APND and ECOD、GST in the hepatopancreas and gills were studied following multi-oral baicalin.

用紫外分光光度法及荧光分光光度法测定中国对虾肝胰腺及鳃氨基比林-N-脱甲基酶、乙氧基香豆素-O-脱乙基酶、谷光甘肽-S-转移酶几种药物代谢酶的活性,探讨黄芩苷对上述几种酶活性的影响。

Methods this experiment chose seven parameter of cell nuclear, including the area and integral optical density, determined the changes of DNA content in the kidney cellule of 15 rats at different intervals between 0 and 48h postmortem with auto-TV-image system.

方法应用电脑图像分析技术,测定了15只大鼠在死后48h内不同时间点大鼠肾细胞核DNA含量的面积、等效直径(Mean-Dia,MD)、异形指数(Indexofdensity,ID)、平均光度(Average optical density,AOD)、积分光度(Integral optical density,IOD)、密度变化数(L-Den-Coe,LDC)和平均灰度(Averagegray,AG)等七项参数的变化值。

One method of preparation new membrane materials was presented with putting multi-wall carbon nanotube and carboxylic multi-wall carbon nanotube in collodion solution.

分别将碳纳米管和羧基化碳纳米管均匀分散在火棉胶溶液中,采用流涎法制备出碳纳米管复合膜;并用分光光度法对样液、滤液中的间苯二酚和茜素红的吸光度进行了对比测定,以考察膜的吸附性能。

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分;测定胸腺指数和脾脏指数;~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应;~3H-TdR参入法测定白细胞介素-1(interleukin-1,IL-1)和IL-2水平;ELISA法测定小鼠血清中抗CⅡ抗体含量;分光光度法检测足爪局部产生的前列腺素水平;HE染色法对关节组织作病理检查。

A modified analysis method based on the determination of ozone—indigo disulphonate spectrophotometer(GB/T 15437-1995) in gas phase has been developed to detect the concentration of ozone in liquid phase. An analytical condition of λmax=612~615 nm,ε=20 000 L/, pH 2 is obtained by the titration of indigo disulphonate. Compared with potassium iodide method,it shows a higher precision and accuracy,CV<3.0%. The results also prove that blank experiment will bring about 5.0%systematic error.

在气相臭氧浓度的靛蓝二磺酸钠分光光度法(GB/T15437-1995)基础上提出了液相臭氧浓度的测定方法,采用靛蓝二磺酸钠标准曲线法代替采用易分解的臭氧标准曲线法,得出测定条件λmax=612~615 nm、ε=20 000 L/、pH为2,通过碘量法比较实验,说明新的测定方法具有较高的精度和准确度,相对标准偏差小于3.0%;比较结果表明忽略空白实验将引入5.0%的系统误差。

Different samples' absorbent was determined in the condition of different colorimetric time ,different samples' temperature and different freshness,it prove that the result was relative to different experiment conditions in order to master the key point and ensuer the accuracy of the result in experiment.

啤酒双乙酰的含量是啤酒质量评价中的一项非常重要的指标,通过对不同样品在不同的比色时间、不同的样品温度及新鲜程度条件下测定其吸光度,得出不同实验条件对测定结果的影响,以求在实验中掌握好其关键点,确保测定结果的准确性。

It is a organic hexahydric weak acid. In different acidity, the dissociation degree and the color of reagent solvent were different also. Determined and draw the absorption curve of reagent in varies acidity, and figured out the dissociation constant by Perisic-Janjic method; the complex ratio of the reagent and bismuth was determinated by mole ratio method and continuous variation method; The apparent stability constant of the complex was calculated, the mechanism of dissociation and coordination were discussed.

2,6—二溴4—硝基偶氮氟胂是本实验室合成的测定铋的一个较好的试剂,对其进行了进一步的研究,在用柱层析法对其进行了提纯后,测定了试剂在不同酸华东师范大学硕:l:学位论文摘要度下的吸光度和最大吸收波长,绘制了其吸收曲线,用Perisic一Janjic法计算了试剂的六级离解常数;用摩尔比法和连续变化法测定了试剂与秘形成的配合物的络合比:计算了配合物的表观稳定常数,研究了其离解和配合机理,为筛选高效有机显色剂提供了理论依据。

The activity of erythrocyte AR and 〓-ATPase, the level of the plasma NO and NOS, the content of sorbitol in sciatic and erythrocyte were detected by spectrophotography; The level of ET in plasma were detected by radioimmunassay .

荧光法测定红细胞AR活性;分光光度法测定红细胞膜〓-ATPase活性、血浆NO及NOS水平、坐骨神经及红细胞内山梨醇含量:放射免疫方法测定血浆ET水平:RT-PCR方法半定量坐骨神经内ARmRNA水平。

These methods include a photocatalytic degradation of methyl orange solution-spectrophotometric method to measure the degradation rate of organic matter by self-cleaning glass, a UV-Vis spectrophotometric scanning procedure to measure the transmittance and a hydrophility measuring method by using a contact angle meter.

详细研究了用以表征自洁净玻璃主要性能的各种测定方法:采用紫外光辐照降解甲基橙-分光光度法测定自洁净玻璃对有机物的降解效率即光催化活性;利用紫外可见光分光光度计扫描测量其透过率光谱曲线;用接触角测定仪测量其样品表面的亲水性。

Solubility of barium chloride and barium oxide in hydrochloric acid, ethanol,acetone,ether and hydrochloric acid- ethanol,hydrochloric acid- acetone,hydrochlo- ric acid- ether has beeen studied and the separation of Ba O from Ba Cl\- 2 with hydrochloric acidethanol as the solvent has been proposed.

研究了氯化钡和氧化钡在盐酸、乙醇、丙酮、乙醚及盐酸-乙醇、盐酸-丙酮、盐酸-乙醚中的溶解性,提出以盐酸-乙醇为溶剂进行两者的分离。以 DBC- CPA为显色剂分光光度法测定氧化钡的含量,加料回收率为 98%~ 10 2 %,氧化钡含量为 0 。32 %时,平行测定 7次,相对标准偏差为 1。 58%。该法适于快速测定热处理盐浴中氧化钡的含量,对监控盐浴品质有指导意义

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